Genome-wide identification of rice class I metallothionein gene: tissue expression patterns and induction in response to heavy metal stress

Gautam, Neelam; Verma, Pankaj; Verma, Shikha; Tripathi, Rudra Deo; Trivedi, Prabodh Kumar; Adhikari, Bijan; Chakrabarty, Debasis

doi:10.1007/s10142-012-0297-9

Cited by 71 publications

(22 citation statements)

References 56 publications

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“…MTs are rich in cysteine residues that coordinate multiple metal atoms such as cadmium, zinc and copper. Eleven MT genes were found to be differentially expressed during growth and development, in various tissues and during biotic and abiotic stresses [84], [85]. Here, 9 of these 11 MT genes were strongly or weakly upregulated in at least one set of conditions (Figure 4B).…”

Section: Resultsmentioning

confidence: 92%

Genome-Wide Transcriptome Analysis Reveals that Cadmium Stress Signaling Controls the Expression of Genes in Drought Stress Signal Pathways in Rice

et al. 2014

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Plant growth is severely affected by toxic concentrations of the non-essential heavy metal cadmium (Cd). Comprehensive transcriptome analysis by RNA-Seq following cadmium exposure is required to further understand plant responses to Cd and facilitate future systems-based analyses of the underlying regulatory networks. In this study, rice plants were hydroponically treated with 50 µM Cd for 24 hours and ∼60,000 expressed transcripts, including transcripts that could not be characterized by microarray-based approaches, were evaluated. Upregulation of various ROS-scavenging enzymes, chelators and metal transporters demonstrated the appropriate expression profiles to Cd exposure. Gene Ontology enrichment analysis of the responsive transcripts indicated the upregulation of many drought stress-related genes under Cd exposure. Further investigation into the expression of drought stress marker genes such as DREB suggested that expression of genes in several drought stress signal pathways was activated under Cd exposure. Furthermore, qRT-PCR analyses of randomly selected Cd-responsive metal transporter transcripts under various metal ion stresses suggested that the expression of Cd-responsive transcripts might be easily affected by other ions. Our transcriptome analysis demonstrated a new transcriptional network linking Cd and drought stresses in rice. Considering our data and that Cd is a non-essential metal, the network underlying Cd stress responses and tolerance, which plants have developed to adapt to other stresses, could help to acclimate to Cd exposure. Our examination of this transcriptional network provides useful information for further studies of the molecular mechanisms of plant adaptation to Cd exposure and the improvement of tolerance in crop species.

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Section: Resultsmentioning

confidence: 92%

Genome-Wide Transcriptome Analysis Reveals that Cadmium Stress Signaling Controls the Expression of Genes in Drought Stress Signal Pathways in Rice

et al. 2014

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“…Rice has 11 class‐I MT genes, and among all MT genes, only one, Os01 g74300 (OsMT‐I‐IIc) (Supplemental Table S4) was upregulated in LARG CN1646‐2. This MT was reported to be upregulated during Cd treatment and several studies showed that MT plays role in maintaining the homeostasis of essential metals and also function in xenobiotics of heavy metals (such as Cd, Hg, Ag, and As) through the thiol group of its cysteine residues (Gautam et al, 2012). Surprisingly, no PCS gene was upregulated in any of the genotypes.…”

Section: Resultsmentioning

confidence: 99%

Comparative Transcriptional Profiling of Contrasting Rice Genotypes Shows Expression Differences during Arsenic Stress

et al. 2015

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Accumulation of arsenic (As) in rice (Oryza sativa L.) grain is a serious concern worldwide. Long-term exposure to As affects nutritional status in rice grain and is associated with higher rates of skin, bladder, and lung cancers, and heart disease. Genotypic variations in rice for As accumulation or tolerance are prevalent and are regulated by genetic and environmental factors. To understand molecular networks involved in As accumulation, genome-wide expression analysis was performed in roots of lowand high-As accumulating rice genotypes (LARGs and HARGs). Six rice genotypes with contrasting As accumulation potential and tolerance were used in this study. Genome-wide expression analysis suggested their differential response against As stress. This study suggests up-and downregulation of a number of unique genes involved in various pathways and biological processes in response to As stress in rice genotypes. A comparison of gene expression profiles, principal component analysis, and K-means clustering suggests that an independent pathway is operating during As stress tolerance or accumulation in contrasting genotypes. It was also observed that the differential behavior of aus genotype, Nayanmoni, from other LARGs might be due to its different genetic background. Cis-motif profiling of As-induced coexpressed genes in diverse rice genotypes led to the identification of unique cis-motifs present in differentially expressed genes. This study suggests that the genetic mechanism regulating the differential As accumulation in different genotypes may not be dependent on gene expression at the transcriptional level. However, many genes identified in this study can be analyzed and used for marker-trait associations related to As accumulation in diverse genotypes around the world.

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“…Furthermore, complexation of PC-arsenite in rice leaves reduces the translocation of arsenic from leaves to grains [97]. In addition, metallothioneins (MTs) also have a potential role in arsenic detoxification in rice, as reported by several authors [118]. Iron (Fe) is an essential element in plants required for various metabolic activities like, photosynthesis, respiration, DNA synthesis, and co-factors for several enzymes.…”

Section: Arsenic Induced Oxidative Stress and Response In Rice Plantmentioning

confidence: 99%

Arsenic Accumulation in Rice and Probable Mitigation Approaches: A Review

Mitra¹,

et al. 2017

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According to recent reports, millions of people across the globe are suffering from arsenic (As) toxicity. Arsenic is present in different oxidative states in the environment and enters in the food chain through soil and water. In the agricultural field, irrigation with arsenic contaminated water, that is, having a higher level of arsenic contamination on the top soil, which may affects the quality of crop production. The major crop like rice (Oryza sativa L.) requires a considerable amount of water to complete its lifecycle. Rice plants potentially accumulate arsenic, particularly inorganic arsenic (iAs) from the field, in different body parts including grains. Different transporters have been reported in assisting the accumulation of arsenic in plant cells; for example, arsenate (As V ) is absorbed with the help of phosphate transporters, and arsenite (As III ) through nodulin 26-like intrinsic protein (NIP) by the silicon transport pathway and plasma membrane intrinsic protein aquaporins. Researchers and practitioners are trying their level best to mitigate the problem of As contamination in rice. However, the solution strategies vary considerably with various factors, such as cultural practices, soil, water, and environmental/economic conditions, etc. The contemporary work on rice to explain arsenic uptake, transport, and metabolism processes at rhizosphere, may help to formulate better plans. Common agronomical practices like rain water harvesting for crop irrigation, use of natural components that help in arsenic methylation, and biotechnological approaches may explore how to reduce arsenic uptake by food crops. This review will encompass the research advances and practical agronomic strategies on arsenic contamination in rice crop.

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Genome-wide identification of rice class I metallothionein gene: tissue expression patterns and induction in response to heavy metal stress

Cited by 71 publications

References 56 publications

Genome-Wide Transcriptome Analysis Reveals that Cadmium Stress Signaling Controls the Expression of Genes in Drought Stress Signal Pathways in Rice

Genome-Wide Transcriptome Analysis Reveals that Cadmium Stress Signaling Controls the Expression of Genes in Drought Stress Signal Pathways in Rice

Comparative Transcriptional Profiling of Contrasting Rice Genotypes Shows Expression Differences during Arsenic Stress

Arsenic Accumulation in Rice and Probable Mitigation Approaches: A Review

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