Genome-Wide Identification of R2R3-MYB Transcription Factors Regulating Secondary Cell Wall Thickening in Cotton Fiber Development

Huang, Junfeng; Guo, Yanjun; Sun, Qianwen; Zeng, Wei; Li, Juan; Li, Xuebao; Xu, Wen‐Liang

doi:10.1093/pcp/pcy238

Cited by 36 publications

(29 citation statements)

References 55 publications

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“…Expression analysis showed that GhCesA genes have overlapping expression profiles and are highly expressed in 20 DPA fibres, although their individual transcript levels vary. Such variations were also found among recently duplicated genes such as GhCesA4‐1 , GhCesA4‐2 , GhCesA4‐3 and GhCesA4‐4 (Huang et al ., 2019). These results indicated that regulation of one GhCesA may differ from that of others and that perhaps different combinations of TFs can regulate each GhCesA .…”

Section: Introductionmentioning

confidence: 99%

“…However, how these TFs control cellulose synthesis remains unknown. We have identified a total of 419 R2R3‐MYBs in the G. hirsutum genome (Huang et al ., 2019). Only a limited number of R2R3‐MYBs, such as GhMYB25, GhMYB25‐like, GhMYB109, GhMYB212, GhMML3_A12, GhMML4_D12, have been characterised functionally in the context of fibre initiation and elongation (Pu et al ., 2008; Machado et al ., 2009; Walford et al ., 2011; Wan et al ., 2016; Wu et al ., 2018; Sun et al ., 2019; Tian et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

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GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis‐elements

et al. 2021

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Summary Cotton fibre is the most important source for natural textiles. The secondary cell walls (SCWs) of mature cotton fibres contain the highest proportion of cellulose content (> 90%) in any plant. The onset and progression of SCW cellulose synthesis need to be tightly controlled to balance fibre elongation and cell wall deposition. However, regulatory mechanisms that control cellulose synthesis during cotton fibre growth remain elusive. Here, we conducted genetic and functional analyses demonstrating that the R2R3‐MYB GhMYB7 controls cotton fibre cellulose synthesis. Overexpression of GhMYB7 in cotton sped up SCW cellulose biosynthesis in fibre cells, and led to shorter fibres with thicker walls. By contrast, RNA interference (RNAi) silencing of GhMYB7 delayed fibre SCW cellulose synthesis and resulted in elongated fibres with thinner walls. Furthermore, we demonstrated that GhMYB7 regulated cotton fibre SCW cellulose synthases by directly binding to three distinct cis‐elements in the respective GhCesA4, GhCesA7 and GhCesA8 promoters. We found that this regulatory mechanism of cellulose synthesis was ‘hi‐jacked’ also by other GhMYBs. Together, our findings uncover a hitherto‐unknown mechanism that cotton fibre employs to regulate SCW cellulose synthesis. Our results also provide a strategy for genetic improvement of SCW thickness of cotton fibre.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis‐elements

et al. 2021

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“…In this study, we identified 393 R2R3-FaMYB genes from the octoploid strawberry genome. This number of R2R3-MYBs is significantly different from the numbers in other species, such as 244 in soybeans ( Aoyagi et al, 2014 ), 192 in the poplar ( Wilkins et al, 2009 ), 256 in the Chinese cabbage ( Wang et al, 2015 ), and 205 in cotton ( Huang et al, 2019 ). This study offers new insights for future investigators to identify functional differences in the R2R3-FaMYB family genes.…”

Section: Discussionmentioning

confidence: 75%

Genome-Wide Analysis of the R2R3-MYB Gene Family in Fragaria × ananassa and Its Function Identification During Anthocyanins Biosynthesis in Pink-Flowered Strawberry

Liu

Wang

et al. 2021

Front. Plant Sci.

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The strawberry (Fragaria × ananassa) is an economically important fruit throughout the world. The large R2R3-MYB gene family participates in a variety of plant functions, including anthocyanin biosynthesis. The present study is the first genome-wide analysis of the MYB gene family in the octoploid strawberry and describes the identification and characterization of the family members using the recently sequenced F. × ananassa genome. Specifically, we aimed to identify the key MYBs involved in petal coloration in the pink-flowered strawberry, which increases its ornamental value. A comprehensive, genome-wide analysis of F. × ananassa R2R3-FaMYBs was performed, investigating gene structures, phylogenic relationships, promoter regions, chromosomal locations, and collinearity. A total of 393 R2R3-FaMYB genes were identified in the F. × ananassa genome and divided into 36 subgroups based on phylogenetic analysis. Most genes with similar functions in the same subgroup exhibited similar exon-intron structures and motif compositions. These R2R3-FaMYBs were unevenly distributed over 28 chromosomes. The expansion of the R2R3-FaMYB gene family in the F. × ananassa genome was found to be caused mainly by segmental duplication. The Ka/Ks analysis indicated that duplicated R2R3-FaMYBs mostly experienced purifying selection and showed limited functional divergence after the duplication events. To elucidate which R2R3-FaMYB genes were associated with anthocyanin biosynthesis in the petals of the pink-flowered strawberry, we compared transcriptional changes in different flower developmental stages using RNA-seq. There were 131 differentially expressed R2R3-FaMYB genes identified in the petals, of which three genes, FaMYB28, FaMYB54, and FaMYB576, appeared likely, based on the phylogenetic analysis, to regulate anthocyanin biosynthesis. The qRT-PCR showed that these three genes were more highly expressed in petals than in other tissues (fruit, leaf, petiole and stolon) and their expressions were higher in red compared to pink and white petals. These results facilitate the clarification on the roles of the R2R3-FaMYB genes in petal coloration in the pink-flowered strawberry. This work provides useful information for further functional analysis on the R2R3-FaMYB gene family in F. × ananassa.

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“…MYB TFs, one of the largest plant TF families, are implicated in the regulation of diverse biological processes in plants, including growth and development (Huang et al, 2019; Zhang et al, 2019), hormone signaling (Kim et al, 2015; Bar et al, 2016), abiotic and biotic stress responses (Shin et al, 2011; Campos et al, 2016; Camargo‐Ramirez et al, 2018), and secondary metabolism (Hu et al, 2017; Ampomah‐Dwamena et al, 2019). Recently, MYB TFs have been reported to play important roles in regulating fruit quality and ripening processes (Medina‐Puche et al, 2015; Wei et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Alternative splicing of MaMYB16L regulates starch degradation in banana fruit during ripening

Jiang

Zhang

et al. 2021

JIPB

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The alternative splicing of select genes is an important mechanism to regulate responses to endogenous and environmental signals in plants. However, the role of alternative splicing in regulating fruit ripening remains unclear. Here, we discovered that MaMYB16L, an R1‐type MYB transcription factor, undergoes alternative splicing and generates two transcripts, the full‐length isoform MaMYB16L and a truncated form MaMYB16S, in banana fruit. During banana fruit ripening, the alternative splicing process intensifies with downregulated MaMYB16L and upregulated MaMYB16S. Moreover, MaMYB16L is a transcriptional repressor that directly binds with the promoters of many genes associated with starch degradation and MaDREB2, a positive ripening regulator, and represses their expression. In contrast, MaMBY16S lacks a DNA‐binding domain but competitively combines and forms non‐functional heterodimers with functional MaMYB16L. MaMYB16L‐MaMYB16S heterodimers decrease the binding capacity and transrepression activity of MaMYB16L. The downregulation of MaMYB16L and the upregulation of MaMYB16S, that is, a decreased ratio of active to non‐active isoforms, facilitates the activation of ripening‐related genes and thereby promotes fruit ripening. Furthermore, the transient overexpression of MaMYB16S promotes banana fruit ripening, whereas the overexpression of MaMYB16L delays this process. Therefore, the alternative splicing of MaMYB16L might generate a self‐controlled regulatory loop to regulate banana fruit ripening.

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Genome-Wide Identification of R2R3-MYB Transcription Factors Regulating Secondary Cell Wall Thickening in Cotton Fiber Development

Cited by 36 publications

References 55 publications

GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis‐elements

GhMYB7 promotes secondary wall cellulose deposition in cotton fibres by regulating GhCesA gene expression through three distinct cis‐elements

Genome-Wide Analysis of the R2R3-MYB Gene Family in Fragaria × ananassa and Its Function Identification During Anthocyanins Biosynthesis in Pink-Flowered Strawberry

Alternative splicing of MaMYB16L regulates starch degradation in banana fruit during ripening

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