Genome-wide Identification of PP2C Genes and Their Expression Profiling in Response to Drought and Cold Stresses in Medicago truncatula

Yang, Qi; Liu, Kun; Niu, Xiaocui; Wang, Qi; Wan, Yongqing; Yang, Feiyun; Li, Guojing; Wang, Yufen; Wang, Ruigang

doi:10.1038/s41598-018-29627-9

Cited by 64 publications

(59 citation statements)

References 63 publications

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“…To study the phylogenetic relationship of the PP2C genes among cotton plants ( G. arboreum , G. barbadense , G. hirsutum , and G. raimondii ), a maximum likelihood (ML) tree was constructed with Arabidopsis using MEGA 7.0. The phylogenetic tree revealed that the PP2C genes can be further divided into 12 subgroups, as previously reported [26,27] (Supplementary Figures S1–S3). Moreover, 181 GhPP2C genes were clustered into 12 subgroups (A–L) with Arabidopsis .…”

Section: Resultssupporting

confidence: 73%

Identification and Expression Profiling of Protein Phosphatases (PP2C) Gene Family in Gossypium hirsutum L.

Shazadee

Khan

Wang

et al. 2019

IJMS

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The protein phosphatase (PP2C) gene family, known to participate in cellular processes, is one of the momentous and conserved plant-specific gene families that regulate signal transduction in eukaryotic organisms. Recently, PP2Cs were identified in Arabidopsis and various other crop species, but analysis of PP2C in cotton is yet to be reported. In the current research, we found 87 (Gossypium arboreum), 147 (Gossypium barbadense), 181 (Gossypium hirsutum), and 99 (Gossypium raimondii) PP2C-encoding genes in total from the cotton genome. Herein, we provide a comprehensive analysis of the PP2C gene family in cotton, such as gene structure organization, gene duplications, expression profiling, chromosomal mapping, protein motif organization, and phylogenetic relationships of each species. Phylogenetic analysis further categorized PP2C genes into 12 subgroups based on conserved domain composition analysis. Moreover, we observed a strong signature of purifying selection among duplicated pairs (i.e., segmental and dispersed) of Gossypium hirsutum. We also observed the tissue-specific response of GhPP2C genes in organ and fiber development by comparing the RNA-sequence (RNA-seq) data reported on different organs. The qRT-PCR validation of 30 GhPP2C genes suggested their critical role in cotton by exposure to heat, cold, drought, and salt stress treatments. Hence, our findings provide an overview of the PP2C gene family in cotton based on various bioinformatic tools that demonstrated their critical role in organ and fiber development, and abiotic stress tolerance, thereby contributing to the genetic improvement of cotton for the resistant cultivar.

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Section: Resultssupporting

confidence: 73%

Identification and Expression Profiling of Protein Phosphatases (PP2C) Gene Family in Gossypium hirsutum L.

Shazadee

Khan

Wang

et al. 2019

IJMS

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“…Further, the homologs of MtPP2C92 and MtPP2C65 in Arabidopsis ( ABI1 and ABI2 , respectively) are the most well-documented PP2C genes that are ABA-dependent and are active under abiotic stresses [ 163 , 165 ]. The MP2C (homolog with AP2C1 ) in alfalfa acts as a negative regulator of the MAPK pathway in cold and drought [ 167 , 168 ]. The AP2C2 (homolog MtPP2C72 ) regulates ROS levels in response to biotic and abiotic stresses [ 168 ].…”

Section: Genes Regulating Ros Homeostasis In Abiotic Stressmentioning

confidence: 99%

“…The MP2C (homolog with AP2C1 ) in alfalfa acts as a negative regulator of the MAPK pathway in cold and drought [ 167 , 168 ]. The AP2C2 (homolog MtPP2C72 ) regulates ROS levels in response to biotic and abiotic stresses [ 168 ]. AP2C1 is strongly induced in response to drought and cold, while AP2C2 is less responsive [ 164 ].…”

Section: Genes Regulating Ros Homeostasis In Abiotic Stressmentioning

confidence: 99%

ROS Homeostasis in Abiotic Stress Tolerance in Plants

Nadarajah

2020

IJMS

361

181

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Climate change-induced abiotic stress results in crop yield and production losses. These stresses result in changes at the physiological and molecular level that affect the development and growth of the plant. Reactive oxygen species (ROS) is formed at high levels due to abiotic stress within different organelles, leading to cellular damage. Plants have evolved mechanisms to control the production and scavenging of ROS through enzymatic and non-enzymatic antioxidative processes. However, ROS has a dual function in abiotic stresses where, at high levels, they are toxic to cells while the same molecule can function as a signal transducer that activates a local and systemic plant defense response against stress. The effects, perception, signaling, and activation of ROS and their antioxidative responses are elaborated in this review. This review aims to provide a purview of processes involved in ROS homeostasis in plants and to identify genes that are triggered in response to abiotic-induced oxidative stress. This review articulates the importance of these genes and pathways in understanding the mechanism of resistance in plants and the importance of this information in breeding and genetically developing crops for resistance against abiotic stress in plants.

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“…Transcriptional profiling showed that microtuber formation in D. opposita is associated with altered transcription state of PP2C, which has both positive and negative roles in ABA signaling [42]. We therefore carried out further studies to elucidate the role of ABA in microtuber formation.…”

Section: Aba Is a Positive Regulator Of Microtuber Formationmentioning

confidence: 99%

Transcriptome Analysis Reveals Key Pathways and Hormone Activities Involved in Early Microtuber Formation of Dioscorea opposita

Zhao

Dong

et al. 2020

BioMed Research International

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Chinese yam (Dioscorea opposita) is an important tuberous crop used for both food and medicine. Despite a long history of cultivation, the understanding of D. opposita genetics and molecular biology remains scant, which has limited its genetic improvement. This work presents a de novo transcriptome sequencing analysis of microtuber formation in D. opposita. We assembled cDNA libraries from different stages during the process of microtuber formation, designated as initial explants (EXP), axillary bud proliferation after three weeks (BUD), and microtuber visible after four weeks (MTV). More differentially expressed genes (DEGs) and pathways were identified between BUD vs. EXP than in MTV vs. BUD, indicating that proliferation of the axillary bud is the key stage of microtuber induction. Gene classification and pathway enrichment analysis showed that microtuber formation is tightly coordinated with primary metabolism, such as amino acid biosynthesis, ribosomal component biosynthesis, and starch and sucrose metabolism. The formation of the microtuber is regulated by a variety of plant hormones, including ABA. Combined with analysis of physiological data, we suggest that ABA positively regulates tuberization in D. opposita. This study will serve as an empirical foundation for future molecular studies and for the propagation of D. opposita germplasm in field crops.

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Genome-wide Identification of PP2C Genes and Their Expression Profiling in Response to Drought and Cold Stresses in Medicago truncatula

Cited by 64 publications

References 63 publications

Identification and Expression Profiling of Protein Phosphatases (PP2C) Gene Family in Gossypium hirsutum L.

Identification and Expression Profiling of Protein Phosphatases (PP2C) Gene Family in Gossypium hirsutum L.

ROS Homeostasis in Abiotic Stress Tolerance in Plants

Transcriptome Analysis Reveals Key Pathways and Hormone Activities Involved in Early Microtuber Formation of Dioscorea opposita

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