Genome-wide identification of plasma membrane aquaporin gene family in Populus and functional identification of PIP1;1 involved in osmotic stress

Jiang, Cheng; Song, Xueqin; He, Hui; Chu, Liwei; Zhou, Houjun; Zhao, Yanqiu; Xu, Yajing; Zeng, Wei; Lin, Xinchun; Lu, Mengzhu

doi:10.1016/j.envexpbot.2020.104200

Cited by 18 publications

(15 citation statements)

References 65 publications

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“…The genes belonging to the PIP were highly expressed. This was consistent with many reports that PIP is known to function in a variety of crops; for example, in Acacia auriculiformis , the AaPIP1-2 gene eliminated ROS and played a positive role in the drop stress; in Populus , the overexpression of PIP1;1 induced more sensitivity of transgenic in osmotic conventions; and in rice, the downregulation of PIP2;1 inhibited root growth [ 26 , 27 , 28 ]. Expression clustering and GO enrichment analyses on the pepper DEGs under abiotic stresses revealed clusters including AQP were generally enriched in GO terms related to the chloroplast, photosystem, organelle, and thylakoid, suggesting that genes involved in these functions may work together with AQP in response to various stresses [ 29 ].…”

Section: Discussionsupporting

confidence: 90%

Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper

Lee

Chae

Kim

et al. 2021

Plants

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Aquaporins (AQPs) are known to have a vital role in water transport in all living organisms including agriculturally important crops, but a comprehensive genomic study of AQPs in pepper has not been implemented. Here, we updated previous gene annotations and generated a total of 259 AQP genes from five plants, including pepper. Phylogenetic and motif analyses revealed that a large proportion of pepper AQP genes belong to the specific subgroup of tonoplast intrinsic protein (TIP) subfamily, TIP4. Chromosomal localization and estimated duplication times illustrated that genes in TIP4 formed a tandem array on the short arm of chromosome 1, resulting from pepper-specific expansion after its divergence with Solanaceae species. Transcriptome analyses under various abiotic stress conditions revealed that transport-, photosystem-, and thylakoid-related genes were generally enriched in expression clusters containing AQP genes in pepper. These results provide valuable genomic resources and insight into the evolutionary mechanism that generate genomic diversity of the AQP gene family in pepper.

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Section: Discussionsupporting

confidence: 90%

Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper

Lee

Chae

Kim

et al. 2021

Plants

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“…However, it should be noted that the turgor pressure estimation method used here was approved in comparison to the direct turgor pressure determination method using the cell-pressure probe, and the result showed good agreement between these two methods [ 52 ]. Interestingly, with the same material, PtPIP1;1 was found to participate in the osmotic stress response [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…It should be noted that in this experiment, PtPIP2,5 and PtPIP2;6 were not included because the sequence similarity was very high, 99%, and no suitable primers were found when we conducted this experiment. However, later, a colleague in our lab found primers to successfully separate the two PIPs and determined that PtPIP2;5 and PtPIP2;6 are the most highly expressed PIPs in poplar roots [ 27 ]. qRT-PCR in this paper was carried out using two-month-old cutting poplar plants growing in a greenhouse, while Jiang et al used tissue culture plantlets [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…However, later, a colleague in our lab found primers to successfully separate the two PIPs and determined that PtPIP2;5 and PtPIP2;6 are the most highly expressed PIPs in poplar roots [ 27 ]. qRT-PCR in this paper was carried out using two-month-old cutting poplar plants growing in a greenhouse, while Jiang et al used tissue culture plantlets [ 27 ]. Despite the different growth conditions of the plants, the qRT-PCR results in this paper are consistent with those of the previous paper.…”

Section: Introductionmentioning

confidence: 99%

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Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development

et al. 2021

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Background Root hydraulic conductance is primarily determined by the conductance of living tissues to radial water flow. Plasma membrane intrinsic proteins (PIPs) in root cortical cells are important for plants to take up water and are believed to be directly involved in cell growth. Results In this study, we found that constitutive overexpression of the poplar root-specific gene PtoPIP1;1 in Arabidopsis accelerated bolting and flowering. At the early stage of the developmental process, PtoPIP1;1 OE Arabidopsis exhibited faster cell growth in both leaves and roots. The turgor pressure of plants was correspondingly increased in PtoPIP1;1 OE Arabidopsis, and the water status was changed. At the same time, the expression levels of flowering-related genes (CRY1, CRY2 and FCA) and hub genes in the regulatory networks underlying floral timing (FT and SOC1) were significantly upregulated in OE plants, while the floral repressor FLC gene was significantly downregulated. Conclusions Taken together, the results of our study indicate that constitutive overexpression of PtoPIP1;1 in Arabidopsis accelerates bolting and flowering through faster cell growth in both the leaf and root at an early stage of the developmental process. The autonomous pathway of flowering regulation may be executed by monitoring developmental age. The increase in turgor and changes in water status with PtoPIP1;1 overexpression play a role in promoting cell growth.

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“…In addition to water transport, AQPs can also transport some small solutes, gases, and metal ions across the membrane [12][13][14][15]. AQPs are involved in leaf stomatal movement [16,17], photosynthesis [18], stress-resistance effectiveness [19][20][21][22][23], and floral opening [24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Aquaporin Gene Family in Pitaya Reveals an HuNIP6;1 Involved in Flowering Process

Gao

Chen

et al. 2021

IJMS

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Aquaporins (AQPs) are essential membrane proteins involved in seed maturation and germination, stomata movement, photosynthesis, and regulation of plant flowering processes. Pitaya flowers are open at night and wither at daybreak, which shows an obvious circadian rhythm. In this study, a comprehensive genome-wide analysis of AQPs in Hylocereus undantus was conducted to screen key genes associated with flowering processes. A total of 33 HuAQP genes were identified from the H. undantus genome. The 33 HuAQPs were grouped into four subfamilies: 10 PIPs, 13 TIPs, 8 NIPs, and 2 SIPs, which were distributed on 9 out of 11 pitaya chromosomes (Chr) (except for Chr7 and Chr10). Results from expression profiles showed that HuNIP6;1 may be involved in pitaya’s floral opening. HuNIP6;1 was localized exclusively in the cell membrane. Overexpression of HuNIP6;1 in Arabidopsis thaliana significantly promoted early flowering through regulating negative flowering regulators of MJM30, COL9, and PRR5, suggesting that HuNIP6;1 plays key roles in regulating flowering time. The present study provides the first genome-wide analysis of the AQP gene family in pitaya and valuable information for utilization of HuAQPs.

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Genome-wide identification of plasma membrane aquaporin gene family in Populus and functional identification of PIP1;1 involved in osmotic stress

Cited by 18 publications

References 65 publications

Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper

Comparative Analysis of Re-Annotated Genes Provides Insight into Evolutionary Divergence and Expressions of Aquaporin Family in Pepper

Poplar aquaporin PIP1;1 promotes Arabidopsis growth and development

Genome-Wide Identification of Aquaporin Gene Family in Pitaya Reveals an HuNIP6;1 Involved in Flowering Process

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