Genome-wide identification of PIP5K in wheat and its relationship with anther male sterility induced by high temperature

Abstract

Background Phosphatidylinositol 4 phosphate 5-kinase (PIP5K) plays a key enzyme role in the inositol signal transduction system and has essential functions in plants in terms of growth, development, and stress responses. However, systematic studies on the wheat PIP5K gene family and its relation to male sterility have not been reported yet. Results Sixty-four TaPIP5K genes were identified. The TaPIP5K genes contained similar gene structures and conserved motifs on the same branches of the evolutionary tree, and their cis-regulatory elements were related to MeJA-responsiveness. Furthermore, 49 pairs of collinearity genes were identified and mainly subjected to purification selection during evolution. Synteny analyses showed that some PIP5K genes in wheat and the other four species shared a relatively conserved evolutionary process. The expression levels of many conservative TaPIP5K genes in HT-ms anthers were significantly lower than that in Normal anthers. In addition, HT-ms anthers have no dehiscence, and levels of OPDA and JA-ILE are significantly lower at the trinucleus stage. Conclusion These results indicate that the PIP5K gene family may be associated with male sterility induced by HT, and the reduction of JA-ILE levels and the abnormal levels of these genes expression may be one reason for the HT-ms anthers having no dehiscence, ultimately leading to the abortion of the anthers.

Genome-wide identification of genes encoding cystathionine beta synthase domain-containing proteins in wheat and its relationship with anther male sterility under heat stress

“…There may be blockages in the production of jasmonic acid, which causes anthers to fail to dehisce, eventually leading to male sterility ( Yang et al., 2020 ). As well, a similar result was demonstrated in our previous study that high temperature significantly reduces levels of OPDA (12-oxo-phytodienoic acid) and JA-ILE (isoleucine jasmonic acid) in male sterile anthers ( Liu et al., 2021b ). In the present study, most of the CBS genes identified had these JA and IAA-related cis-acting elements in the upstream region ( Figure 6 ), suggesting that the expression of CBS genes is closely related to these cis-acting elements, which may have a relationship with high temperature induced male sterility.…”

“…MEGA-X software was used to construct a phylogenetic tree of the CBS gene family from the above plant species and wheat. The parameters were set as previously described ( Liu et al., 2021b ). Detailed parameters for phylogenetic tree construction: Algorithm of Neighbor-Joining, poission correction, and bootstrap repeated value 1000 times.…”

“…To observe the TaCBS gene expression in different wheat tissues, the wheat RNA-Seq data were downloaded from WheatOmics 1.0 (http://wheatomics.sdau.edu.cn/) as depicted in a previous study (Liu et al, 2021b;Ma et al, 2021). TBtools software was used to perform log normalization on the downloaded TPM (Transcripts Per Kilobase Million) values, and to draw the expression heatmap of wheat CBS genes.…”

Genetic diversity and local adaption of alfalfa populations (Medicago sativa L.) under long-term grazing

“…It has been reported that the peptidyl prolyl cis–trans-Isomerase FKBP77 gene is involved in heat stress tolerance 55 . The PI monophosphate 5-kinase (PIP5K) enzyme containing membrane occupation and recognition nexus motif is involved in heat and drought-responses in plants 56 , 57 .…”

Genetic Diversity and Local Adaption of Alfalfa Populations (Medicago sativa L.) under Long-Term Grazing

“…It has been reported that the peptidyl prolyl cis-trans-Isomerase FKBP77 gene is involved in heat stress tolerance 55 . The PI monophosphate 5-kinase (PIP5K) enzyme containing membrane occupation and recognition nexus motif is involved in heat and drought-responses in plants 56, 57 .…”