Genome-wide identification of papain-like cysteine protease family genes in cultivated peanut (Arachis hypogaea L.) and functional characterization of AhRD21B in response to chilling stress

Zhang, Chong; Chen, Yuting; Wang, Lihui; Liu, Lu; Zhong, Xin; Chu, Panpan; Gao, Meijia; Chen, Hua; Cai, Tiecheng; Xiong, Fei; Zhang, Xiurong; Raza, Ali; Pan, Rong-Long; Varshney, Rajeev K.; Liu, Fengzhen; Zhuang, Weijian

doi:10.1016/j.envexpbot.2023.105272

Cited by 2 publications

(1 citation statement)

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“…Several plant species, including cotton, cus, peanut, and orange, contain genes encoding for papain-like (PLCP) cysteine proteases of the C1A subfamily (Zhang et al 2019;Zhai et al 2021;Zhang et al 2023;Li et al 2023). Species have different numbers of C1A protease genes.…”

Section: Discussionmentioning

confidence: 99%

Genome wide identification of bromelain-like cysteine proteases in Puya raimondii

Castro-Cabrera,

Torre,

Chandía

et al. 2024

Preprint

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Bromelains are cysteine proteases of the papain family (C1A subfamily). These enzymes are of great commercial value due to their applications in the food, pharmaceutical and biotechnological industries. In plants, they play an important role in multiple physiological processes from germination to senescence, mainly in the defense of plants against biotic and abiotic stresses. In this study, we used available data from the P. raimondii genome (Bioproject PRJNA639677) to identify genes encoding bromelain-type proteases. Six bromelain-like nucleotide sequences are postulated in P. raimondii draft genome. Hormone, stress and light responsive elements in the PrBLCPs sequences were detected. The theoretical molecular weights of the proteins putatives PrBLCPs in P. raimondii range from 36643.21-45761.20 Da and theoretical isoelectric point 5.05 to 8.63. Multiple primary sequence alignments and structural model prediction demonstrate that P. raimondii putative proteases are very similar to A. comosus fruit bromelain (O23791) recognized in the SwissProt/Uniprot database. Phylogenetic relationships between the bromelain-like putative cysteine proteases of P. raimondii and the bromelain proteases of A. comosus placed the PrBLCPs in two subclades, although with very short phylogenetic distances between them. The results of this study provide valuable information for future genomic studies of bromelain family genes in the genus Puya. In addition, it aids in the understanding of the regulatory mechanisms of these proteases and their roles in plant stress tolerance.

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Section: Discussionmentioning

confidence: 99%