Genome-Wide Identification of NAC Transcription Factor Family and Functional Analysis of the Abiotic Stress-Responsive Genes in Medicago sativa L.

Min, Xueyang; Jin, Xiaoyu; Zhang, Zhengshe; Wei, Xingyi; Ndayambaza, Boniface; Wang, Yanrong; Liu, Wenxian

doi:10.1007/s00344-019-09984-z

Cited by 25 publications

(17 citation statements)

References 82 publications

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“…The 162 CsNAC proteins varied from 157 (CsNAC004) to 856 (CsNAC076) amino acid residues with an average of 378.5 aa. The relative Mws ranged from 17.8 (CsNAC004) to 96.1 (CsNAC076) kDa with an average of 41.8 kDa, and the pIs ranged from 4.48 (CsNAC003) to 11.3 (CsNAC063) with 101 members showing isoelectric point (pI) ≤ 7 and the remainders showing pI > 7 ( Table S1 ), suggesting that NAC TFs may act under a different physiological environment [ 10 ].…”

Section: Resultsmentioning

confidence: 99%

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Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Zong

et al. 2020

Genes

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Plant-specific NAC (NAM, ATAF, CUC) transcription factor (TF) family plays important roles in biological processes such as plant growth and response to stress. Nevertheless, no information is known about NAC TFs in Cleistogenes songorica, a prominent xerophyte desert grass in northwestern China. In this study, 162 NAC genes were found from the Cleistogenes songorica genome, among which 156 C. songoricaNAC (CsNAC) genes (96.3%) were mapped onto 20 chromosomes. The phylogenetic tree constructed by CsNAC and rice NAC TFs can be separated into 14 subfamilies. Syntenic and Ka/Ks analyses showed that CsNACs were primarily expanded by genomewide replication events, and purifying selection was the primary force driving the evolution of CsNAC family genes. The CsNAC gene expression profiles showed that 36 CsNAC genes showed differential expression between cleistogamous (CL) and chasmogamous (CH) flowers. One hundred and two CsNAC genes showed differential expression under heat, cold, drought, salt and ABA treatment. Twenty-three CsNAC genes were commonly differentially expressed both under stress responses and during dimorphic floret development. Gene Ontology (GO) annotation, coexpression network and qRT-PCR tests revealed that these CsNAC genes may simultaneously regulate dimorphic floret development and the response to stress. Our results may help to characterize the NAC transcription factors in C. songorica and provide new insights into the functional research and application of the NAC family in crop improvement, especially in dimorphic floret plants.

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Section: Resultsmentioning

confidence: 99%

“…Furthermore, the relatively divergent C-terminal transcriptional regulation region of NAC TFs can repress or activate the transcription of multiple target genes [ 8 ]. In addition, some NAC proteins possess α-helical transmembrane motifs (TMs) at their C-terminus for binding to the plasma membrane [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Zong

et al. 2020

Genes

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“…Abiotic stress is an important factor that threatens the yield and quality of strawberry. To alleviate the damage of abiotic stress, plants usually initiate complex adaptation via genetic mechanisms including regulation of gene expression and increased concentration of osmolytes [5,27]. There are several reports revealing that the ATAF sub-group of TFs belonging to the NAC family play important roles in response to abiotic stress; however, their function is still under debate.…”

Section: Fanac2 Functions As a Positive Regulator In Response To Abiomentioning

confidence: 99%

“…To adapt to environmental stress and complete their life cycle, plants have evolved a complex mechanism that tightly regulates gene expression through precise signaling. Until now, many stress-induced proteins including transcription factors (TFs), osmotic stress-adaptive proteins and key enzymes in abscisic acid (ABA) biosynthesis and signaling pathways have been reported [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…The NAC [No apical meristem (NAM), Arabidopsis transcription activation factor (ATAF), and Cup-shaped cotyledon (CUC)] superfamily is one of the largest groups of plant-specific TFs, which not only play an important role in various stages of plant growth and development, but also participate in responses to biotic and abiotic stress [6][7][8][9][10]. Many NAC genes have been identified through their function in response to drought, cold, and high salinity stress [5]. Drought-induced genes ANAC019, ANAC055, and RD26/ANAC072, when overexpressed in Arabidopsis, improved drought tolerance [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Strawberry FaNAC2 Enhances Tolerance to Abiotic Stress by Regulating Proline Metabolism

Liang

Zheng

et al. 2020

Plants

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The quality and yields of strawberry plants are seriously affected by abiotic stress every year. NAC (NAM, ATAF, CUC) transcription factors are plant-specific, having various functions in plant development and response to stress. In our study, FaNAC2 from strawberry (Fragaria × ananassa, cultivar “Benihoppe”) was isolated and found to be a member of the ATAF sub-family, belonging to the NAC family of transcription factors. FaNAC2 was strongly expressed in the shoot apical meristem and older leaves of strawberries, and was induced by cold, high salinity, and drought stress. To investigate how FaNAC2 functions in plant responses to abiotic stress, transgenic Nicotiana benthamiana plants ectopically overexpressing FaNAC2 were generated. The transgenic plants grew better under salt and cold stress, and, during simulated drought treatment, these transgenic lines not only grew better, but also showed higher seed germination rates than wild-type plants. Gene expression analysis revealed that key genes in proline biosynthesis pathways were up-regulated in FaNAC2 overexpression lines, while its catabolic pathway genes were down-regulated and proline was accumulated more with the overexpression of FaNAC2 after stress treatments. Furthermore, the gene expression of abscisic acid biosynthesis was also promoted. Our results demonstrate that FaNAC2 plays an important positive role in response to different abiotic stresses and may be further utilized to improve the stress tolerance of strawberry plants.

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Transcription Factors in Alfalfa (Medicago sativa L.): Genome-Wide Identification and a Web Resource Center AlfalfaTFDB

et al. 2021

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Genome-Wide Identification of NAC Transcription Factor Family and Functional Analysis of the Abiotic Stress-Responsive Genes in Medicago sativa L.

Cited by 25 publications

References 82 publications

Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Genome-Wide Analysis of the Role of NAC Family in Flower Development and Abiotic Stress Responses in Cleistogenes songorica

Strawberry FaNAC2 Enhances Tolerance to Abiotic Stress by Regulating Proline Metabolism

Transcription Factors in Alfalfa (Medicago sativa L.): Genome-Wide Identification and a Web Resource Center AlfalfaTFDB

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