Genome-wide identification of mitogen-activated protein kinase gene family in Gossypium raimondii and the function of their corresponding orthologs in tetraploid cultivated cotton

Zhang, Xueying; Wang, Liman; Xu, Xiaoyang; Cai, Caiping; Guo, Wangzhen

doi:10.1186/preaccept-3285223921360401

Cited by 10 publications

(22 citation statements)

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“…Since the nature of protein kinase activity depends on their direct physical encounters, we conducted a comprehensive directed yeast two-hybrid (Y2H) assay to define the interactions between the last two kinases in the MAPK cascade in cotton. Besides the 28 MAPK genes reported previously 36 , we further confirmed 56, 52 and 28 MAPK genes from G. hirsutum , G. barbadense and G. arboreum , respectively ( Supplementary Dataset 2 ). Due to the high similarity (more than 97%) in the amino acid sequences ( Supplementary Table 2 ) of MKK and MAPK homoeologs in the A- and D-subgenomes, we performed PCR-based cloning for anyone of the MKK homoeologs.…”

Section: Resultssupporting

confidence: 84%

“…In total, eight MKK genes were cloned and sequenced to confirm their complete open reading frame (GenBank accession numbers: KX118695-KX118702) ( Table 1 ). Combined with the 21 MAPK genes cloned previously 36 , we constructed eight MKKs and 21 MAPKs into DNA binding domain and activation domain plasmid vectors, respectively. Each MKK protein was found to have a distinct auto-activation activity in yeast.…”

Section: Resultsmentioning

confidence: 99%

“…A growing body of evidence suggests that there are fewer MKKs than MAPKKKs and MAPKs, suggesting that individual MKKs may function as bifurcation points and are likely to be involved in multiple MAPK cascades in response to a variety of stresses 34 . In addition to those described above, a total of 78 MAPKKK genes and 28 MAPK genes have been identified in the G. raimondii genome 35 36 . In spite of this, the systematical investigation of the MKK gene families in cotton and the verification of the interactions between MKK and MAPK members in MAPK cascades have not been studied.…”

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confidence: 99%

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Integration analysis of MKK and MAPK family members highlights potential MAPK signaling modules in cotton

Zhang

et al. 2016

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Mitogen-activated protein kinase (MAPK) cascades play a crucial role in plant growth and development, as well as their biotic and abiotic stress responses. As a nodal point of the MAPK cascade, the MKK gene family has not been systematically studied in cotton. Here, we identified 11 putative MKK genes in the Gossypium raimondii genome. Phylogenetic analysis showed that the MKKs were supported by architectures of conserved protein motifs. Expression patterns of MKKs under hormone treatments or abiotic stresses revealed their diverse functions in stress responses. Based on a yeast two hybrid, a total of 63 interactive pairs of MKKs and MAPKs were identified in cotton. Among these, 40 interactive pairs were newly identified compared to that reported previously in Arabidopsis. Integration analysis of the interaction network and expression patterns of MKK and MAPK family members revealed 13 potential MAPK signaling modules that are involved in the complicated cross-talk between hormones and abiotic stresses. Taken together, our data enhance the understanding of the evolution and function of MAPK cascades in cotton, and lay the foundation for the improvement of various defense responses that use MAPK signaling modules in the future.

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Integration analysis of MKK and MAPK family members highlights potential MAPK signaling modules in cotton

Zhang

et al. 2016

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“…Agrobacterium cultures containing recombinant TRV vectors were prepared and then infiltrated into two fully expanded cotyledons of eight-day-old seedlings, as previously described by Zhang et al . 57 . The seedlings were cultivated in a greenhouse at 23/22 °C (day/night) with a 16 h light/8 h dark photoperiod.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide characterization of the β-1,3-glucanase gene family in Gossypium by comparative analysis

Feng

Fang

et al. 2016

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The β-1,3-glucanase gene family is involved in a wide range of plant developmental processes as well as pathogen defense mechanisms. Comprehensive analyses of β-1,3-glucanase genes (GLUs) have not been reported in cotton. Here, we identified 67, 68, 130 and 158 GLUs in four sequenced cotton species, G. raimondii (D5), G. arboreum (A2), G. hirsutum acc. TM-1 (AD1), and G. barbadense acc. 3–79 (AD2), respectively. Cotton GLUs can be classified into the eight subfamilies (A–H), and their protein domain architecture and intron/exon structure are relatively conserved within each subfamily. Sixty-seven GLUs in G. raimondii were anchored onto 13 chromosomes, with 27 genes involved in segmental duplications, and 13 in tandem duplications. Expression patterns showed highly developmental and spatial regulation of GLUs in TM-1. In particular, the expression of individual member of GLUs in subfamily E was limited to roots, leaves, floral organs or fibers. Members of subfamily E also showed more protein evolution and subgenome expression bias compared with members of other subfamilies. We clarified that GLU42 and GLU43 in subfamily E were preferentially expressed in root and leaf tissues and significantly upregulated after Verticillium dahliae inoculation. Silencing of GLU42 and GLU43 significantly increased the susceptibility of cotton to V. dahliae.

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“…Little is known about the relationship between PRODH and the MAPK pathway in plants, however, hypoosmotic stress was observed to induce a similar pattern of MPK20 and PRODH expression in Arabidopsis , suggesting a link between PRODH and MPK20 ( Moustafa et al, 2008 ). A recent genomic study showed MPK20 was highly induced by H 2 O 2 stress in cotton ( Gossypium raimondii , Zhang et al, 2014 ). Is it plausible that ROS generated by proline metabolism induces MPK20 during petal senescence?…”

Section: Proline In Senescing Petals: Energy Depletion and Ros Accumumentioning

confidence: 99%

Connecting proline metabolism and signaling pathways in plant senescence

2015

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The amino acid proline has a unique biological role in stress adaptation. Proline metabolism is manipulated under stress by multiple and complex regulatory pathways and can profoundly influence cell death and survival in microorganisms, plants, and animals. Though the effects of proline are mediated by diverse signaling pathways, a common theme appears to be the generation of reactive oxygen species (ROS) due to proline oxidation being coupled to the respiratory electron transport chain. Considerable research has been devoted to understand how plants exploit proline metabolism in response to abiotic and biotic stress. Here, we review potential mechanisms by which proline metabolism influences plant senescence, namely in the petal and leaf. Recent studies of petal senescence suggest proline content is manipulated to meet energy demands of senescing cells. In the flower and leaf, proline metabolism may influence ROS signaling pathways that delay senescence progression. Future studies focusing on the mechanisms by which proline metabolic shifts occur during senescence may lead to novel methods to rescue crops under stress and to preserve post-harvest agricultural products.

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Genome-wide identification of mitogen-activated protein kinase gene family in Gossypium raimondii and the function of their corresponding orthologs in tetraploid cultivated cotton

Cited by 10 publications

References 50 publications

Integration analysis of MKK and MAPK family members highlights potential MAPK signaling modules in cotton

Integration analysis of MKK and MAPK family members highlights potential MAPK signaling modules in cotton

Genome-wide characterization of the β-1,3-glucanase gene family in Gossypium by comparative analysis

Connecting proline metabolism and signaling pathways in plant senescence

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