Genome-wide identification of lncRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

Zhu, Bin; Xu, Manyu; Shi, Haiyan; Gao, Xiwu; Liang, Pei

doi:10.1186/s12864-017-3748-9

Cited by 71 publications

(96 citation statements)

References 51 publications

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“…The global control and damage costs for this insect pest are estimated at US $4–5 billion per year . Insecticide is the most effective tool for P. xylostella control, but due to its irrational usage, P. xylostella has developed resistance to various types of insecticides and has become one of the most resistant pests in the world . Therefore, novel management strategies are urgently required .…”

Section: Introductionmentioning

confidence: 99%

Identification and RNAi‐based function analysis of chitinase family genes in diamondback moth, Plutella xylostella

Zhu

Shan

et al. 2019

Pest Management Science

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BACKGROUND: Insect chitinases play a vital part in chitin degradation in exoskeletons and gut linings during the molting process, and therefore are considered potential targets for new insecticide designs or RNA interference (RNAi)-based pest management. Systematic functional analysis of chitinase genes has already been conducted in several insect pests, but not Plutella xylostella. RESULTS:In this study, 13 full-length chitinase transcripts were obtained in P. xylostella. Developmental and tissue-specific expression pattern analysis revealed that seven chitinase transcripts were periodically expressed during molting stage and mainly expressed in the integument or midgut, including PxCht3, PxCht5, PxCht6-2, PxCht7, PxCht8, PxCht10 and PxCht-h. RNAi-mediated knockdown of these specific expressed genes revealed that PxCht5 and PxCht10 were essential in larval molting, pupation and eclosion, and PxCht7 was indispensable only in eclosion. No significant effects were observed on insect survival or normal development when the rest chitinase transcripts were suppressed by RNAi. CONCLUSION: Our results indicated the function of P. xylostella chitinase family genes during the molting process, and may provide potential targets for RNAi-based management of P. xylostella.

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Section: Introductionmentioning

confidence: 99%

Identification and RNAi‐based function analysis of chitinase family genes in diamondback moth, Plutella xylostella

Zhu

Shan

et al. 2019

Pest Management Science

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“…Furthermore, based on positional conservation in chromosome and sequence conservation analysed by the web Blastn program at the National Center for Biotechnology Information (NCBI), 210 lncRNAs with potential human orthologs were screened for follow‐up verification, including 129 up‐regulated and 81 down‐regulated (Figure C and 1D). The 210 lncRNAs can be classified into four categories including ‘u' (intergenic), ‘i' (intronic), ‘x' (anti‐sense) and ‘o' (sense overlapping) according to their genomic location and referring to the neighbouring genes . Most of the identified lncRNAs fell into class i, with 101 lncRNAs (48.09%), whereas 69 (32.86%), 18 (8.57%) and 22 (10.48%) lncRNAs belonged to classes i, x and o, respectively (Figure E).…”

Section: Resultsmentioning

confidence: 99%

Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout

Zhang

Jiang

Cui

et al. 2019

J Cellular Molecular Medi

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Recent studies have demonstrated that aberrant long non‐coding RNAs (lncRNAs) expression are suggested to be closely associated with multiple human diseases, lung cancer included. However, the roles of lncRNAs in lung cancer are not well understood. In this study, we used microarrays to investigate the aberrantly expressed lncRNAs in the mouse lung adenocarcinoma with P53 knockout and the KrasG12D mutation. Results revealed that 6424 lncRNAs were differentially expressed (≥ 2‐fold change, P < .05). Two hundred and ten lncRNAs showed more than 8‐fold change and conserved across human and were further analysed in the primary mouse lung adenocarcinoma KP cells, which were isolated from the p53 knockout and the KrasG12D mutation mice. Among all the 210 lncRNAs, 11 lncRNAs' expression was regulated by P53, 33 lncRNAs by KRAS and 13 lncRNAs by hypoxia in the primary KP cells, respectively. NONMMUT015812, which was remarkably up‐regulated in the mouse lung adenocarcinoma and negatively regulated by the P53 re‐expression, was detected to analyse its cellular function. Results showed that knockdown of NONMMUT015812 by shRNAs decreased proliferation and migration abilities of KP cells. Among those aberrantly expressed lncRNAs in the mouse lung adenocarcinoma, NONMMUT015812 was a potential oncogene.

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“…In conclusion, of 57 miRNAs identified in S. avenae, the transcript levels of 16 Table S1. Toxicity of imidacloprid on Sitobion avenae Table S2.…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed microRNAs under imidacloprid exposure in Sitobion avenae Fabricius

Zhang

Xie

et al. 2020

Preprint

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Background: MicroRNAs (miRNAs), which are short single-stranded non-coding RNAs, regulate the expression of target genes, especially those involved in the regulation or metabolism of endogenous or xenobiotic compounds. Results: De novo assemblies of the transcriptome of Sitobion avenae Fabricius under control conditions and under imidacloprid treatment were obtained using Illumina short-read sequencing technology. Fifty-seven miRNAs, of which 36 were known and 21 were novel, were identified. Quantitative analysis of miRNA levels showed that 5 miRNAs were significantly up-regulated, and 11 miRNAs were significantly down-regulated in the nymphs of S. avenae treated with imidacloprid in comparison with those of the control. Analysis of the candidate target genes in S. avenae that could be regulated by these miRNAs were also carried out. The functions of the miRNAs, which could potentially regulate target genes that participate in metabolism, regulatory or detoxification pathways in S. avenae, were clarified based on Gene Ontology and KEGG pathway analysis. The effects of the miRNAs api-miR-1000, api-miR-316, and api-miR-iab-4 on susceptibility of S. avenae to imidacloprid was determined. Modulation of the abundances of api-miR-1000, api-miR-316, and api-miR-iab-4 by the addition of the correspondign inhibitors to the artificial diet significantly changed the susceptibility of S. avenae to imidacloprid, which further demonstrated the effect of these miRNAs on the regulation or metabolism of insecticides.Conclusion: The results of this study suggested that miRNAs differentially expressed in response to imidacloprid could play a critical regulatory role in the resistance of S. avenae to imidacloprid.

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Genome-wide identification of lncRNAs associated with chlorantraniliprole resistance in diamondback moth Plutella xylostella (L.)

Cited by 71 publications

References 51 publications

Identification and RNAi‐based function analysis of chitinase family genes in diamondback moth, Plutella xylostella

Identification and RNAi‐based function analysis of chitinase family genes in diamondback moth, Plutella xylostella

Deregulated lncRNA expression profile in the mouse lung adenocarcinomas with KRAS‐G12D mutation and P53 knockout

Identification of differentially expressed microRNAs under imidacloprid exposure in Sitobion avenae Fabricius

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