Genome-wide identification of JNK and p38 gene family in Ctenopharyngodon idella and their expression profiles in response to bacterial challenge

Xu, Xiaoyan; Tao, Lizhu; Wang, Anqi; Li, Liuyang; Fan, Kebin; Shen, Yubang; Li, Jiale

doi:10.1016/j.cbd.2019.100647

Cited by 4 publications

(4 citation statements)

References 36 publications

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“…P-loop has a glycine-rich GXGXXG motif, which is the binding site of ATP and GTP. 44,45 Meanwhile, C-loop has the D[L/I/V]KP[G/S/ K]N motif and contains an Asp (D) residue, which is the key phosphorylation site. 44,45 Activate-loop contains a phosphorylation-activation TXY motif.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Sep38β has three conserved motifs: P-loop, C-loop, and activate-loop. P-loop has a glycine-rich GXGXXG motif, which is the binding site of ATP and GTP. , Meanwhile, C-loop has the D[L/I/V]KP[G/S/K]N motif and contains an Asp (D) residue, which is the key phosphorylation site. , Activate-loop contains a phosphorylation-activation TXY motif. − The amino acid sequences of p38βs in Lepidoptera and Hymenoptera were shown to be highly consistent, with the three motifs exhibiting identical sequences (Figure ).…”

Section: Discussionmentioning

confidence: 99%

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Involvement of Sep38β in the Insecticidal Activity of Bacillus thuringiensis against Beet Armyworm, Spodoptera exigua (Lepidoptera)

Ji,

Gao,

Zhao

et al. 2024

J. Agric. Food Chem.

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The p38 mitogen-activated protein kinases (MAPKs) are associated with insect immunity, tissue repair, and the insecticidal activity of Bacillus thuringiensis (Bt). Here, a p38 MAPK family gene (Sep38β) was identified from Spodoptera exigua. Among the developmental stages, the transcription level of Sep38β was the highest in egg, followed by that in prepupa and pupa. Sep38β expression peaked in Malpighian tubules and the hemolymph of fifth instar larvae. Knockdown of Sep38β or injection of SB203580 (a p38 MAPK inhibitor) significantly downregulated the SeDUOX expression and reactive oxygen species (ROS) level in the midgut, accounting for deterioration of the midgut to scavenge pathogens and enhancement of Bt insecticidal activity. In conclusion, all the results demonstrate that Sep38β regulates the immune-related ROS level in the insect midgut, which suppresses the insecticidal activity of Bt against S. exigua by 17−22%. Our study highlights that Sep38β is essential for insect immunity and the insecticidal activity of Bt to S. exigua and is a potential target for pest control.

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Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Involvement of Sep38β in the Insecticidal Activity of Bacillus thuringiensis against Beet Armyworm, Spodoptera exigua (Lepidoptera)

Ji,

Gao,

Zhao

et al. 2024

J. Agric. Food Chem.

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“…qPCR was conducted using the following program: 95 • C for 5 min, 40 cycles of 95 • C for 5 s, 60 • C for 20 s, and 72 • C for 20 s, followed by melting curve determination from 65 • C to 95 • C to verify the amplification of a single product. The relative expression levels of the target genes were measured by the 2 −∆∆Ct method [65], and 18S rRNA was used as the internal control [66][67][68][69]. The relative expression levels were indicated as fold change.…”

Section: Qpcr Analysismentioning

confidence: 99%

Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R

Wang

Sun

Zhang

et al. 2022

IJMS

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Interleukin-6 (IL-6) is a multipotent cytokine. IL-6 plays a dual role in inflammation through both classical signaling (IL-6 binds membrane IL-6 receptor/IL-6R) and trans-signaling (IL-6 binds soluble IL-6R). However, the regulation of IL-6 activity, especially the regulation of signaling pathways and downstream genes mediated by IL-6 trans-signaling, remains largely unclear in teleost. Grass carp (Ctenopharyngodon idellus) hepatic (L8824) cells, kidney (CIK) cells, and primary hepatocytes were used as test models in this study. First, the biological activity of recombinant blunt snout bream (Megalobrama amblycephala) IL-6 (rmaIL-6) and sIL-6R (rmasIL-6R) was verified by quantitative PCR (qPCR) and western blot. The western blot results showed that rmaIL-6 significantly upregulated signal transducer and activator of transcription 3 (STAT3) phosphorylation in L8824 cells and primary hepatocytes, while rmaIL-6 in combination with rmasIL-6R (rmaIL-6+rmasIL-6R) significantly upregulated STAT3 phosphorylation in all types of cells. Furthermore, maIL-6 and maIL-6+rmasIL-6R could only induce extracellular-signal-regulated kinase 1/2 (ERK1/2) phosphorylation in L8824 cells and CIK cells, respectively. Therefore, IL-6 mainly acts by activating the janus kinase (JAK)/STAT3 pathway rather than the mitogen-activated protein kinase (MEK)/ERK pathway. Finally, the activation of the JAK2/STAT3 pathway was shown to be essential for the generation of socs3a and socs3b induced by IL-6 trans-signaling after treatment by JAK2/STAT3 pathway inhibitors (c188-9 and TG101348). These findings provide functional insights into IL-6 classical signaling and trans-signaling regulatory mechanisms in teleost, enriching our knowledge of fish immunology.

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“…In addition, the p38 s are essential for cells in releasing related pro‐inflammatory cytokines, whereas JNK s are key regulators of immune injuries in cells (Shi et al ., 2019; Zhang, Chen, et al, 2019). Rather than ERK , JNK and p38 pathways take responsibility for controlling the activity and expression of certain vital mediators of inflammation, such as cytokines and proteases (Xu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of mitogen‐activated protein kinase (MAPK) gene family in yellow catfish (Pelteobagrus fulviadraco) and their expression profiling under the challenge of Aeromonas hydrophila

Shu

Liu

et al. 2022

Journal of Fish Biology

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As serine/threonine protein kinases, mitogen-activated protein kinases (MAPK) take part in cellular metabolism. This work found 14 MAPK genes in the yellow catfish (Pelteobagrus fulviadraco) genome and evaluated their taxonomy, conserved domains and evolutionary linkages for a better understanding of the MAPK gene family's evolutionary relationship and antibacterial immune response. The findings revealed that several MAPK genes are activated in response to immunological and inflammatory responses. Collinearity research revealed that in yellow catfish and zebrafish, there are six pairs of highly similar MAPK genes, indicating that these genes have been more conserved throughout evolution. The MAPK gene quantification findings revealed that JNK1a, JNK1b, p38delta and p38alpha b expression levels were considerably upregulated, indicating that they act in fish innate immunity. The findings implied that MAPK genes may involve in defence against detrimental microbe in yellow catfish, which will help researchers better understand how MAPK genes work in the innate immune system.

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Genome-wide identification of JNK and p38 gene family in Ctenopharyngodon idella and their expression profiles in response to bacterial challenge

Cited by 4 publications

References 36 publications

Involvement of Sep38β in the Insecticidal Activity of Bacillus thuringiensis against Beet Armyworm, Spodoptera exigua (Lepidoptera)

Involvement of Sep38β in the Insecticidal Activity of Bacillus thuringiensis against Beet Armyworm, Spodoptera exigua (Lepidoptera)

Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R

Genome‐wide identification of mitogen‐activated protein kinase (MAPK) gene family in yellow catfish (Pelteobagrus fulviadraco) and their expression profiling under the challenge of Aeromonas hydrophila

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