Genome-wide identification of GH3 genes in Brassica oleracea and identification of a promoter region for anther-specific expression of a GH3 gene

Abstract

Background The Gretchen Hagen 3 (GH3) genes encode acyl acid amido synthetases, many of which have been shown to modulate the amount of active plant hormones or their precursors. GH3 genes, especially Group III subgroup 6 GH3 genes, and their expression patterns in economically important B. oleracea var. oleracea have not been systematically identified. Results As a first step to understand regulation and molecular functions of Group III subgroup 6 GH3 genes, 34 GH3 genes including four subgroup 6 genes were identified in B. oleracea var. oleracea. Synteny found around subgroup 6 GH3 genes in B. oleracea var. oleracea and Arabidopsis thaliana indicated that these genes are evolutionarily related. Although expression of four subgroup 6 GH3 genes in B. oleracea var. oleracea is not induced by auxin, gibberellic acid, or jasmonic acid, the genes show different organ-dependent expression patterns. Among subgroup 6 GH3 genes in B. oleracea var. oleracea, only BoGH3.13–1 is expressed in anthers when microspores, polarized microspores, and bicellular pollens are present, similar to two out of four syntenic A. thaliana subgroup 6 GH3 genes. Detailed analyses of promoter activities further showed that BoGH3.13–1 is expressed in tapetal cells and pollens in anther, and also expressed in leaf primordia and floral abscission zones. Conclusions Sixty-two base pairs (bp) region (− 340 ~ − 279 bp upstream from start codon) and about 450 bp region (− 1489 to − 1017 bp) in BoGH3.13–1 promoter are important for expressions in anther and expressions in leaf primordia and floral abscission zones, respectively. The identified anther-specific promoter region can be used to develop male sterile transgenic Brassica plants.

Transcriptome differential expression analysis of defoliation of two different lemon varieties

“…Transcriptome analysis of lychee after ring-stripping defoliation treatment showed that the expression of LcAUX/IAA1 increased in the fruitlet AZ, suggesting that it may have an important role in the abscission process ( Kuang et al, 2012 ). Brassica oleracea BoGH3.13-1 is expressed in chorioallantoic cells, pollen, the leaf primordium, and in the off-zone of flower abscission, suggesting its possible involvement in these physiological processes ( Jeong et al, 2021 ). There are 29 genes related to hormones such as growth hormone, abscisic acid, and ethylene that are differentially expressed in the abscission microsomes of citrus in the off-region transcriptome, including a family of genes related to growth hormone response, including GH3 ( Xie et al, 2018 ).…”

Genome-wide identification and expression characterization of the GH3 gene family of tea plant (Camellia sinensis)

“…The GH3 gene family was first discovered and named by Gretchen Hagen in soybean ( Glycine max ). Currently, there have been reports of the genome-wide identification of the GH3 gene family, not only in model plants such as Arabidopsis [ 28 ] and tobacco, but also in various other plant species, including cucumber [ 49 ], cabbage [ 50 ], walnut [ 51 ], and kiwifruit [ 52 ]. Indole-3-acetic acid (IAA) is of crucial importance for plant growth and development.…”

Comprehensive Analysis of GH3 Gene Family in Potato and Functional Characterization of StGH3.3 under Drought Stress

“…The expression analysis of GH3 gene in different tissues of different plant species at different developmental stages shows that these genes play different roles in plants [11]. A large number of studies on the expression pattern of GH3 genes in different plants have provided rich references for analyzing GH3 in potato [18,57]. TaGH3 was found to be expressed mainly in the roots and leaves and in different amounts [7], while OsGH3.2 [58] was highly expressed in roots.…”