Genome-wide identification of cassava MeRboh genes and functional analysis in Arabidopsis

Huang, Siyuan; Tang, Zhijuan; Zhao, Rui; Hong, Yi; Zhu, Shousong; Fan, Ruochen; Ding, Kaixuan; Cao, Min; Luo, Kai; Geng, Meiyu; Jiang, Lingyan; Chen, Yinhua

doi:10.1016/j.plaphy.2021.07.039

Cited by 18 publications

(19 citation statements)

References 42 publications

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“…Prediction programs for subcellular localization showed that the seven CsRboh proteins were localized in the cell membrane. The results are in agreement with those obtained in other studies [ 10 , 13 , 14 , 16 , 28 ], suggesting Rbohs’ potential role in the regulation of ROS generation. Notably, few Rboh proteins were predicted to localize in chloroplast thylakoid membranes or mitochondria.…”

Section: Discussionsupporting

confidence: 93%

“…Here, we identified a total of seven Rboh genes from the Citrus sinensis genome ( Table 1 ). The number of Rboh genes in Citrus is similar to previous reports of grapes [ 8 ], strawberries [ 12 ], jatropha [ 13 ], cassava [ 14 ], and pepper [ 16 ], but relatively small. Prediction programs for subcellular localization showed that the seven CsRboh proteins were localized in the cell membrane.…”

Section: Discussionsupporting

confidence: 87%

“…Rbohs are encoded by multiple genes, and the gene family has been identified and characterized in some plant species. So far, ten Rboh genes were found in Arabidopsis [ 6 ], nine in rice [ 7 ], seven in grapes [ 8 ], fourteen in Nicotiana tabacum [ 9 ], twenty-six in upland cotton [ 10 ], nine in apples [ 11 ], seven in strawberries [ 12 ], seven in jatropha [ 13 ], eight in cassava [ 14 ], fourteen in Brassica rapa [ 15 ], and eight in pepper [ 16 ]. Analysis of gene expression patterns may help to understand the possible function of Rboh family genes.…”

Section: Introductionmentioning

confidence: 99%

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Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Zhang

Luo

et al. 2022

IJMS

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Respiratory burst oxidase homologs (Rbohs) are critical enzymes involved in the generation of reactive oxygen species (ROS) that play an important role in plant growth and development as well as various biotic and abiotic stresses in plants. Thus far, there have been few reports on the characterization of the Rboh gene family in Citrus. In this study, seven Rboh genes (CsRbohA~CsRbohG) were identified in the Citrus sinensis genome. The CsRboh proteins were predicted to localize to the cell membrane. Most CsRbohs contained four conserved domains, an EF-hand domain, and a transmembrane region. Phylogenetic analysis demonstrated that the CsRbohs were divided into five groups, suggesting potential distinct functions and evolution. The expression profiles revealed that these seven CsRboh genes displayed tissue-specific expression patterns, and five CsRboh genes were responsive to cold stress. Fourteen putative cis-acting elements related to stress response, hormone response, and development regulation were present within the promoters of CsRboh genes. The in-silico microRNA target transcript analyses indicated that CsRbohE might be targeted by csi-miR164. Further functional and physiological analyses showed that the knockdown of CsRbohD in trifoliate orange impaired resistance to cold stress. As a whole, our results provide valuable information for further functional studies of the CsRboh genes in response to cold stress.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Zhang

Luo

et al. 2022

IJMS

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“…The homozygotes of T3 plants were used for detecting the Pst DC3000 sensitivity. Then, 4-week-old transgenic Arabidopsis plants were inoculated with Pst DC3000 as previously described (Huang et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

MeWRKY IIas, Subfamily Genes of WRKY Transcription Factors From Cassava, Play an Important Role in Disease Resistance

et al. 2022

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Cassava (Manihot esculenta Crantz) is an important tropical crop for food, fodder, and energy. Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) occurs in all cassava growing regions and threatens global cassava production. WRKY transcription factor family plays the essential roles during plant growth, development, and abiotic or biotic stress. Particularly, previous studies have revealed the important role of the group IIa WRKY genes in plant disease resistance. However, a comprehensive analysis of group IIa subfamily in cassava is still missing. Here, we identified 102 WRKY members, which were classified into three groups, I, II, and III. Transient expression showed that six MeWRKY IIas were localized in the nucleus. MeWRKY IIas transcripts accumulated significantly in response to SA, JA, and Xam. Overexpression of MeWRKY27 and MeWRKY33 in Arabidopsis enhanced its resistance to Pst DC3000. In contrast, silencing of MeWRKY27 and MeWRKY33 in cassava enhanced its susceptibility to Xam. Co-expression network analysis showed that different downstream genes are regulated by different MeWRKY IIa members. The functional analysis of downstream genes will provide clues for clarifying molecular mechanism of cassava disease resistance. Collectively, our results suggest that MeWRKY IIas are regulated by SA, JA signaling, and coordinate response to Xam infection.

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“…In addition to its role in inducing RNAi, dsRNA has been reported to be an elicitor of pattern triggered immunity (PTI; [ 15 , 16 , 17 ]). RNA-based pathogen-associated molecular patterns (PAMPs) are well-known inducers of immunity in both animals [ 18 , 19 , 20 , 21 ], and plants [ 15 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Impact of Exogenous Application of Potato Virus Y-Specific dsRNA on RNA Interference, Pattern-Triggered Immunity and Poly(ADP-ribose) Metabolism

Samarskaya

Spechenkova

Markin

et al. 2022

IJMS

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In this work we developed and exploited a spray-induced gene silencing (SIGS)-based approach to deliver double-stranded RNA (dsRNA), which was found to protect potato against potato virus Y (PVY) infection. Given that dsRNA can act as a defence-inducing signal that can trigger sequence-specific RNA interference (RNAi) and non-specific pattern-triggered immunity (PTI), we suspected that these two pathways may be invoked via exogeneous application of dsRNA, which may account for the alterations in PVY susceptibility in dsRNA-treated potato plants. Therefore, we tested the impact of exogenously applied PVY-derived dsRNA on both these layers of defence (RNAi and PTI) and explored its effect on accumulation of a homologous virus (PVY) and an unrelated virus (potato virus X, PVX). Here, we show that application of PVY dsRNA in potato plants induced accumulation of both small interfering RNAs (siRNAs), a hallmark of RNAi, and some PTI-related gene transcripts such as WRKY29 (WRKY transcription factor 29; molecular marker of PTI), RbohD (respiratory burst oxidase homolog D), EDS5 (enhanced disease susceptibility 5), SERK3 (somatic embryogenesis receptor kinase 3) encoding brassinosteroid-insensitive 1-associated receptor kinase 1 (BAK1), and PR-1b (pathogenesis-related gene 1b). With respect to virus infections, PVY dsRNA suppressed only PVY replication but did not exhibit any effect on PVX infection in spite of the induction of PTI-like effects in the presence of PVX. Given that RNAi-mediated antiviral immunity acts as the major virus resistance mechanism in plants, it can be suggested that dsRNA-based PTI alone may not be strong enough to suppress virus infection. In addition to RNAi- and PTI-inducing activities, we also showed that PVY-specific dsRNA is able to upregulate production of a key enzyme involved in poly(ADP-ribose) metabolism, namely poly(ADP-ribose) glycohydrolase (PARG), which is regarded as a positive regulator of biotic stress responses. These findings offer insights for future development of innovative approaches which could integrate dsRNA-induced RNAi, PTI and modulation of poly(ADP-ribose) metabolism in a co-ordinated manner, to ensure a high level of crop protection.

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Genome-wide identification of cassava MeRboh genes and functional analysis in Arabidopsis

Cited by 18 publications

References 42 publications

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

Genome Wide Identification of Respiratory Burst Oxidase Homolog (Rboh) Genes in Citrus sinensis and Functional Analysis of CsRbohD in Cold Tolerance

MeWRKY IIas, Subfamily Genes of WRKY Transcription Factors From Cassava, Play an Important Role in Disease Resistance

Impact of Exogenous Application of Potato Virus Y-Specific dsRNA on RNA Interference, Pattern-Triggered Immunity and Poly(ADP-ribose) Metabolism

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