Genome-Wide Identification of BES1 Gene Family in Six Cucurbitaceae Species and Its Expression Analysis in Cucurbita moschata

Abstract

The BES1 (BRI1-EMSSUPPRESSOR1) gene family play a vital role in the BR (brassinosteroid) signaling pathway, which is involved in the growth and development, biotic, abiotic, and hormone stress response in many plants. However, there are few reports of BES1 in Cucurbita moschata. In this study, 50 BES1 genes were identified in six Cucurbitaceae species by genome-wide analysis, which could be classified into 3 groups according to their gene structural features and motif compositions, and 13 CmoBES1 genes in Cucurbita moschata were mapped on 10 chromosomes. Quantitative real-time PCR analysis showed that the CmoBES1 genes displayed differential expression under different abiotic stress and hormone treatments. Subcellular localization showed that the most of CmoBES1 proteins localized in nucleus and cytoplasm, and transactivation assay indicated 9 CmoBES1 proteins played roles as transcription factors. Our analysis of BES1s diversity, localization, and expression in Curcubitaceae contributes to the better understanding of the essential roles of these transcription factors in plants.

Genome-Wide Identification of Vascular Plant One-Zinc-Finger Gene Family in Six Cucurbitaceae Species and the Role of CmoVOZ2 in Salt and Drought Stress Tolerance

“…In this study, pumpkin plants were cultivated in Hoagland’s nutrient solution at a greenhouse under controlled conditions (28 °C, 16 h light/8 h dark, 70–80% humidity). Upon attaining the three-leaf stage, the seedlings underwent three treatments as described in [ 36 ]: ① leaves of hormone treatment groups (100 µM ABA, MeJA, and GA) for 0, 3, 6, and 9 h were collected separately for RNA extraction; ② leaves were exposed to drought (20% PEG6000, Solarbio, Beijing, China) for 6 h; and ③ leaves were exposed to salt (150 mM NaCl, Solarbio, Beijing, China) for 6 h to evaluate separately the responsiveness of CmoVOZ genes to abiotic stresses. Three biological replicates were set up for each sample.…”

Genome-wide identification and in-silico expression analysis of carotenoid cleavage oxygenases gene family in Oryza sativa (rice) in response to abiotic stress

“…Still, due to the absence of CCDL proteins, it is not an ideal candidate for investigating CCD enzymes and their functions. Fortunately, CCDL genes have been discovered in Citrullus lanatus and Cucumis melo, providing opportunities for further exploration (Xu et al, 2023). Carotenoids provide vibrant colors to fruits and vegetables, and their production and degradation are facilitated by CCD enzyme family (Kiokias et al, 2016).…”