“…In this study, pumpkin plants were cultivated in Hoagland’s nutrient solution at a greenhouse under controlled conditions (28 °C, 16 h light/8 h dark, 70–80% humidity). Upon attaining the three-leaf stage, the seedlings underwent three treatments as described in [ 36 ]: ① leaves of hormone treatment groups (100 µM ABA, MeJA, and GA) for 0, 3, 6, and 9 h were collected separately for RNA extraction; ② leaves were exposed to drought (20% PEG6000, Solarbio, Beijing, China) for 6 h; and ③ leaves were exposed to salt (150 mM NaCl, Solarbio, Beijing, China) for 6 h to evaluate separately the responsiveness of CmoVOZ genes to abiotic stresses. Three biological replicates were set up for each sample.…”