Genome-wide identification, genomic organization, and expression profiling of the CONSTANS-like (COL) gene family in petunia under multiple stresses

Abstract

Background CONSTANS-like (CO-like, COL) are putative zinc-finger transcription factors known to play vital role in various plant biological processes such as control of flowering time, regulation of plant growth and development and responses to stresses. However, no systematic analysis of COL family gene regarding the plant development and stress response has been previously performed in any solanaceous crop. In the present study, a comprehensive genome-wide analysis of COL family genes in petunia has been conducted to figure out their roles in development of organs and stress response. Results A total of 33 COL genes, 15 PaCOL genes in P. axillaris and 18 PiCOL genes in P. inflata, were identified in petunia. Subsequently, a genome-wide systematic analysis was performed in 15 PaCOL genes. Considering the domain composition and sequence similarity the 15 PaCOL and 18 PiCOL genes were phylogenetically classified into three groups those are conserved among the flowering plants. Moreover, all of the 15 PaCOL proteins were localized in nucleus. Furthermore, differential expression patterns of PaCOL genes were observed at different developmental stages of petunia. Additionally, transcript expression of 15 PaCOL genes under various abiotic and phytohormone treatments showed their response against stresses. Moreover, several cis-elements related to stress, light-responsive, hormone signaling were also detected in different PaCOL genes. Conclusion The phylogenetic clustering, organ specific expression pattern and stress responsive expression profile of conserved petunia COL genes indicating their involvement in plant growth and development and stress response mechanism. This work provide a significant foundation for understanding the biological roles of petunia COL genes in plant growth, development and in stress response.

Genome-wide identification of the mango CONSTANS (CO) family and functional analysis of two MiCOL9 genes in transgenic Arabidopsis

“…In this study, we analyzed cis-elements in the 2000 bp promoter regions of MiCOL genes. These genes mainly included light, circadian control, hormone and stress-responsive elements and were similar to genes in the NtCOL gene family (Zhang et al, 2021), PaCOL gene family (Khatun et al, 2021) and PtCOL gene family (Li et al, 2020a). The results imply that MiCOL genes might be involved in light, circadian control, and hormone and stress response pathways in mango.…”

“…The results showed that most of the MiCOL genes were mainly expressed in leaves and had high expression levels during the floral induction and differentiation periods. Similarly, 9 PtCOL genes are predominantly expressed in leaves (Li et al., 2020), 5 PaCOL genes are preferentially expressed in stems ( Khatun et al., 2021 ), and BnaCO. A10 and BnaCO.…”

“…The Ghd7 gene, a homolog of the CO - like gene, has been reported to regulate stress tolerance in rice ( Liu et al., 2016 ). In the PaCOL gene family, the expression of 3 genes is upregulated significantly after heat, cold and drought stresses, and that of the PaCOL2 gene is sharply elevated under salt conditions ( Khatun et al., 2021 ). In this experiment, the expression levels of six genes were altered significantly after salt treatment, and seven genes were upregulated after drought treatment.…”

MiCOL6, MiCOL7A and MiCOL7B isolated from mango regulate flowering and stress response in transgenic Arabidopsis

“…In the present study, MiCOL7A and MiCOL7B were expressed mainly in 20 DAF fruits, and MiCOL6 , MiCOL7A and MiCOL7B exhibited increased expression during the flowering induction and flowering of mango plants. In grape, nine PtCOL s were more highly expressed in leaves, while nine PaCOL genes were preferentially expressed in stems of Platanus acerifolia (Li et al, 2020; Khatun et al, 2021). In our previous study, MiCO , MiCOL1A , MiCOL16A and MiCOL16B were shown to be highly expressed in leaves and during flower‐forming induction and floral bud differentiation periods (Liu et al, 2020; Guo et al, 2022; Liu et al, 2022a).…”

Worldwide Selection Footprints for Drought and Heat in Bread Wheat (Triticum aestivum L.)

“…The most frequent loci on chromosomes 1A and 4D are related to two genes: CONSTANS-like and proteins abundant in late embryogenesis (LEA). The first is involved in various biological processes of plants, such as the control of the flowering time, regulation of growth and development, and responses to abiotic stress [32][33][34]. LEA proteins are recognized during the adaptation to abiotic stress, which includes dehydration, salinity, high temperature, and cold [35][36][37].…”