2021
DOI: 10.1038/s41598-021-87991-5
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Genome-wide identification, evolutionary relationship and expression analysis of AGO, DCL and RDR family genes in tea

Abstract: Three gene families in plants viz. Argonaute (AGOs), Dicer-like (DCLs) and RNA dependent RNA polymerase (RDRs) constitute the core components of small RNA mediated gene silencing machinery. The present study endeavours to identify members of these gene families in tea and to investigate their expression patterns in different tissues and various stress regimes. Using genome-wide analysis, we have identified 18 AGOs, 5 DCLs and 9 RDRs in tea, and analyzed their phylogenetic relationship with orthologs of Arabido… Show more

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Cited by 15 publications
(10 citation statements)
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References 70 publications
(58 reference statements)
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“…dsRNAs are a potent trigger for cellular pathogen defence pathways of plant cells, namely RNA interference. The Arabidopsis genome contains six RDRs linked to the silencing of distinct target RNAs ( Willmann et al , 2011 ), but the copy number varies across plant species ( Krishnatreya et al , 2021 ). RDR activity can also start from ssRNA, where it can be primed by 22-nucleotide small RNAs (sRNAs), resulting in secondary sRNA production from an initial sRNA trigger for the cellular amplification of silencing ( Lopez-Gomollon and Baulcombe, 2022 ).…”
Section: Rna Productionmentioning
confidence: 99%
“…dsRNAs are a potent trigger for cellular pathogen defence pathways of plant cells, namely RNA interference. The Arabidopsis genome contains six RDRs linked to the silencing of distinct target RNAs ( Willmann et al , 2011 ), but the copy number varies across plant species ( Krishnatreya et al , 2021 ). RDR activity can also start from ssRNA, where it can be primed by 22-nucleotide small RNAs (sRNAs), resulting in secondary sRNA production from an initial sRNA trigger for the cellular amplification of silencing ( Lopez-Gomollon and Baulcombe, 2022 ).…”
Section: Rna Productionmentioning
confidence: 99%
“…This finding suggests that sRNAs are selectively loaded by host plants into EVs during pathogen infection. In eukaryotes, Argonaute (AGO) proteins bind selectively to specific sRNAs and then guide the RNA‐induced silencing complex to corresponding target genes by complementary base pairing between the target mRNA and the sRNA guide strand (Krishnatreya et al, 2021 ; Liu et al, 2017 ). To elucidate the mechanism underlying selective loading of RNAs into EVs by plants, He et al ( 2021 ) used proteomic analysis to identify various RNA‐binding proteins in Arabidopsis EVs; these included AGO1, DEAD‐box RNA helicases (RH11, RH37), and annexins (ANN1, ANN2).…”
Section: Roles Of Evs In Plant–fungus Interactionsmentioning
confidence: 99%
“…To date, several studies have shown that the sizes of AGO , DCL , and RDR gene families vary among species. For example, Arabidopsis 14 , rice 15 , maize 16 , millet 17 , grapevine 18 , tomato 19 , wheat 20 , soybean 21 , pepper 22 , cucumber 23 , barley 24 , sugarcane 25 , sweet orange 6 , and tea 26 genomes encode ten, 19, 18, 19, 13, 15, 39, 21, 12, seven, 11, 21, eight, and 18 AGO genes; four, five, five, eight, four, seven, seven, seven, four, five, five, four, four, and five DCL genes; six, eight, five, 11, five, six, 16, seven, six, eight, seven, 11, four, and nine RDR genes, respectively. These studies have also shown that these gene families are highly conserved in plants, although little is known about the corresponding genes in quinoa.…”
Section: Introductionmentioning
confidence: 99%