Genome-Wide Identification, Evolutionary Patterns, and Expression Analysis of bZIP Gene Family in Olive (Olea europaea L.)

Abstract

Olive (Olea europaea.L) is an economically important oleaginous crop and its fruit cold-pressed oil is used for edible oil all over the world. The basic region-leucine zipper (bZIP) family is one of the largest transcription factors families among eukaryotic organisms; its members play vital roles in environmental signaling, stress response, plant growth, seed maturation, and fruit development. However, a comprehensive report on the bZIP gene family in olive is lacking. In this study, 103 OebZIP genes from the olive genome were identified and divided into 12 subfamilies according to their genetic relationship with 78 bZIPs of A. thaliana. Most OebZIP genes are clustered in the subgroup that has a similar gene structure and conserved motif distribution. According to the characteristics of the leucine zipper region, the dimerization characteristics of 103 OebZIP proteins were predicted. Gene duplication analyses revealed that 22 OebZIP genes were involved in the expansion of the bZIP family. To evaluate the expression patterns of OebZIP genes, RNA-seq data available in public databases were analyzed. The highly expressed OebZIP genes and several lipid synthesis genes (LPGs) in fruits of two varieties with different oil contents during the fast oil accumulation stage were examined via qRT-PCR. By comparing the dynamic changes of oil accumulation, OebZIP1, OebZIP7, OebZIP22, and OebZIP99 were shown to have a close relationship with fruit development and lipid synthesis. Additionally, some OebZIP had a significant positive correlation with various LPG genes. This study gives insights into the structural features, evolutionary patterns, and expression analysis, laying a foundation to further reveal the function of the 103 OebZIP genes in olive.

Genome-Wide Identification and Expression Analysis of the Strawberry FvbZIP Gene Family and the Role of Key Gene FabZIP46 in Fruit Resistance to Gray Mold

“…Although the functions of most of the motif elements were unknown, certain motifs were observed in specific bZIP members, suggesting that these transcription factors had specific functions. A similar finding has been reported in other species, indicating that some elements are relatively conserved evolutionarily [ 27 ]. These unknown elements may have specific functions in each subfamily, which may provide a new reference for the functional divergence among members of each subfamily.…”

Genome-wide characterization of bZIP gene family identifies potential members involved in flavonoids biosynthesis in Ginkgo biloba L.

“…Therefore, the genetic information of G. biloba is of great research value in plant growth and development, pest and disease defense, abiotic stress, and tree evolution. To date, the bZIP gene family has been identified in A. thaliana (78 bZIPs) 30 , Populus (86 bZIPs) 37 , Raphanus sativus (135 bZIPs) 15 , Solanum tuberosum (80 bZIPs) 38 , Brassica napus (247 bZIPs) 38 , and Olea europaea (103 bZIPs) 39 . However, the bZIP transcription factors in G. biloba have not been reported yet.…”

A Review of Nervonic Acid Production in Plants: Prospects for the Genetic Engineering of High Nervonic Acid Cultivars Plants

“…Previous studies suggested that MYB and bZIP transcription factors participated in regulating the synthesis of FAs ( Yamamoto et al, 2009 ; Rong et al, 2020 ), and MYB played important roles in VLCFA biosynthesis particularly ( Raffaele et al, 2008 ). A weighted gene co-expression network analysis in Acer truncatum Bunge ( A. truncatum ) showed that MYB and bZIP transcription factors were involved in regulating NA biosynthesis ( Ma et al, 2020 ).…”