Genome-wide identification, evolution, and transcriptome-based expression profiling analysis of suppressors of cytokine signaling (SOCS) in grass carp (Ctenopharyngodon idella)

Xu, Tianbing; Su, Jian

doi:10.1016/j.aquaculture.2021.736484

Cited by 4 publications

(4 citation statements)

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“…The position of all spotted seabass SOCS genes on the locus, the order of upstream and downstream genes, and exon-intron structure similar to SOCSs in other species [35]. For example, spotted seabass SOCS1 and SOCS3a are found on the same chromosome (chromosome 12), and grass carp SOCS1 and SOCS3a were also on chromosome 2 [14], suggesting a close functional relationship between SOCS1 and SOCS3a. However, further investigation is needed to determine whether the functions of other closely located genes, such as SOCS2 and SOCS3b (chromosome 24) and SOCS7 and CISH (chromosome 2), are similar.…”

Section: Lateolabrax Maculatus Chr4mentioning

confidence: 78%

“…However, further investigation is needed to determine whether the functions of other closely located genes, such as SOCS2 and SOCS3b (chromosome 24) and SOCS7 and CISH (chromosome 2), are similar. The majority of spotted seabass SOCSs genes have 1-3 exons, except for SOCS7, which is also found in all other fish [11,14,20,36]. Therefore, it can be concluded that the gene structure of SOCSs in fish is highly conserved throughout the course of evolution.…”

Section: Lateolabrax Maculatus Chr4mentioning

confidence: 96%

“…Phylogenetic analysis shows that the vertebrate SOCS gene family can be divided into two subfamilies: the type I subfamily consists of vertebrate SOCS4, SOCS5a, SOCS5b, SOCS6a, SOCS6b, SOCS7, and SOCS9. The type II subfamily is composed of CISH, SOCS1a, SOCS1b, SOCS2, SOCS3a, SOCS3b, and SOCS8 [12][13][14]. The way in which SOCS proteins are grouped together reflects their evolutionary history, with proteins that share similar structures and functions being clustered together [15].…”

Section: Introductionmentioning

confidence: 99%

“…To date, SOCS family members have been identified in a variety of fish species, in addition to the five model fish mentioned above, also including rainbow trout [16], channel catfish [17], tongue sole [18], nile tilapia [19], Japanese flounder [20], soiny mullet [5], yellow catfish [21], swamp eel [22], grass carp [14], and redlip mullet [8]. However, little is known about studies in Perciformes.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Expression Analysis of Suppressors of Cytokine Signaling from Spotted Seabass (Lateolabrax maculatus)

Cai,

Xie,

Gao

et al. 2024

Aquaculture Research

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The suppressor of cytokine signaling (SOCS) plays a negative role in the cytokine signaling pathway, preventing excessive signaling from interfering with the metabolic homeostasis of the body. By regulating the Janus kinase-signal transducer and activator of transcription pathway through negative feedback, SOCS have a significant impact on the regulation of both innate and adaptive immunity against pathogens, thus playing a crucial role in the immune response, growth, and development of the body. In this study, the cDNA sequences of SOCS1, 2, 3a, 3b, 4, 5b, 6, 7, 8, 9, and CISH genes of spotted seabass (Lateolabrax maculatus), an important marine economic fish in China, were cloned using RT-PCR, nested PCR, and RACE techniques. Multiple sequence alignment showed that the SOCS family members shared highly conserved functional structural domains, including the SRC homology 2 domain (SH2 domain) and the SOCS-box domain. The phylogenetic analysis showed that SOCS1, 2, 3a, 3b, 8, and CISH belonged to the type II subfamily of SOCS genes, while SOCS4, 5b, 6, 7, and SOCS9 belonged to the type I subfamily. Furthermore, gene organization and syntenic analysis confirmed the phylogenetic analysis and protein annotation of the SOCS gene family in spotted seabass. Constitutive expression of spotted seabass SOCS genes was observed in various tissues of healthy fish, with varying expression levels. Following the lipopolysaccharide and Edwardsiella tarda challenge, the expression profiles of spotted seabass SOCS genes were differently regulated in the gill, head kidney, intestine, and spleen. These findings provide a basis for future research on the functional properties of SOCS genes in spotted seabass.

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Section: Lateolabrax Maculatus Chr4mentioning

confidence: 78%