Genome-wide identification, evolution analysis of cytochrome P450 monooxygenase multigene family and their expression patterns during the early somatic embryogenesis in Dimocarpus longan Lour.

Zhang, Chunyu; Xu, Xiaoqiong; Xu, Xiaoping; Li, Yang; Zhao, Pengcheng; Chen, Xiaohui; Xu, Shihong; Zhang, Zihao; Chen, Yukun; Liu, Shengcai; XuHan, Xu; Lin, Yuling; Zhang, Lai

doi:10.1016/j.gene.2022.146453

Cited by 15 publications

(13 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytochromes P450 (CYPs, CYP450, and P450) are heme-containing enzymes. They are known as monooxygenases catalyzing oxygenation/hydroxylation reactions in the biosynthesis of many secondary metabolites [ 59 , 60 ]. The microarray analysis of 326 CYP genes in the rice genome displayed tissue-specific and zone-specific expression patterns.…”

Section: The Involvement Of Mirna In Plant Lignan Biosynthesismentioning

confidence: 99%

“…It has been confirmed that the expression of CYP genes can be post-transcriptionally regulated by miRNAs families, such as miR440, miR531, miR812, and by species-specific families miR1848, miR5523 (rice genome) [ 59 ]. It has been elucidated that CYPs involved in the phenylpropanoid pathway are primarily regulated and targeted by miR413 [ 60 ]. A Cytochrome P450 has been found to be a target for miR5035, miR2275d [ 61 ], and for lus-miR168b [ 62 ].…”

Section: The Involvement Of Mirna In Plant Lignan Biosynthesismentioning

confidence: 99%

“… The schematic representation of the lignans biosynthesis indicating the known points of the pathway in which the miRNAs participate (Ražná, Harenčár, elaborated based on [ 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 ]. …”

Section: Figurementioning

confidence: 99%

See 2 more Smart Citations

The Involvement of microRNAs in Plant Lignan Biosynthesis—Current View

Ražná¹,

Harenčár²,

Kučka³

2022

Cells

View full text Add to dashboard Cite

Lignans, as secondary metabolites synthesized within a phenylpropanoid pathway, play various roles in plants, including their involvement in growth and plant defense processes. The health and nutritional benefits of lignans are unquestionable, and many studies have been devoted to these attributes. Although the regulatory role of miRNAs in the biosynthesis of secondary metabolites has been widely reported, there is no systematic review available on the miRNA-based regulatory mechanism of lignans biosynthesis. However, the genetic background of lignan biosynthesis in plants is well characterized. We attempted to put together a regulatory mosaic based on current knowledge describing miRNA-mediated regulation of genes, enzymes, or transcription factors involved in this biosynthesis process. At the same time, we would like to underline the fact that further research is necessary to improve our understanding of the miRNAs regulating plant lignan biosynthesis by exploitation of current approaches for functional identification of miRNAs.

show abstract

Section: The Involvement Of Mirna In Plant Lignan Biosynthesismentioning

confidence: 99%

Section: The Involvement Of Mirna In Plant Lignan Biosynthesismentioning

confidence: 99%

See 1 more Smart Citation

The Involvement of microRNAs in Plant Lignan Biosynthesis—Current View

Ražná¹,

Harenčár²,

Kučka³

2022

Cells

View full text Add to dashboard Cite

show abstract

“…Recently, plant hormone signalling-related genes, especially the auxin and cytokinin signalling components, were reported to be signi cantly enriched in early SE of hybrid sweetgum (Liquidambar styraci ua × Liquidambar formosana) and four modules of genes were identi ed with properties relating to embryonic potential, early somatic embryogenesis, and somatic embryo development [10]. Core cell cycle genes, cytochrome P450 genes and polyamines have been shown to play an important regulatory role during the early SE of Dimocarpus longan Lour [11][12][13]. However, proteins involved in the SE and early SE of Eucalyptus remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Proteome profiles of two Eucalyptus species during the early stage of somatic embryogenesis

Chen

et al. 2022

Preprint

View full text Add to dashboard Cite

BackgroundEarly somatic embryogenesis (SE) has been recognized as an important tool for plants to propagate in vitro. However, our knowledge about the proteins involved in early SE including the callus dedifferentiation is still limited, especially in the economic woody tree – Eucalyptus.ResultsWe performed the data-independent acquisition (DIA) mass-spectrometry (MS) to study the proteins involved in early SE of two Eucalyptus species - E. camaldulensis (high regeneratively potential) and E. grandis x urophylla (low regenerative potential). Initially, the data-dependent acquisition MS produced 44,451 peptides and 9,142 proteins. While the DIA MS identified 35,207 peptides and 7,077 proteins in the stem and tissue-culture induced callus of the two Eucalyptus species. MSstat identified 2,078 and 2,807 differentially expressed proteins (DEPs) in early SE of E. camaldulensis and E. grandis x urophylla, respectively. They shared 760 upregulated and 420 downregulated proteins, including 4 transcription factors, 31 ribosomal proteins, 1 histone, 3 zinc finger proteins (ZFPs), ARF19, WOX8 and PIN1. Further, 27 and 4 DEPs were found to be involved in “photosynthesis” (ko00195) and “two-component system” (ko02020), respectively. Notably, SERK, WRKY, ZFP and ABC transporter proteins were analyzed to be related with high SE potential. By combining the miRNA and RNA-Seq results, some miRNA-mRNA/protein regulation networks might be involved in early SE of Eucalyptus, like miR160~TPP2, miR164~UXS2, miR169~COX11 and miR535~ Eucgr.E01067.ConclusionsThis is the first study of proteome profiles in early SE of Eucalyptus. The findings will improve our understanding of molecular changes and regulatory networks in early SE of Eucalyptus.

show abstract

“…Recently, plant hormone signalling-related genes, especially the auxin and cytokinin signalling components, were reported to be significantly enriched in early SE of hybrid sweetgum (Liquidambar styraciflua × Liquidambar formosana) and four modules of genes were identified with properties relating to embryonic potential, early somatic embryogenesis, and somatic embryo development [10]. Core cell cycle genes, cytochrome P450 genes and polyamines have been shown to play an important regulatory role during the early SE of Dimocarpus longan Lour [11][12][13]. However, our knowledge about Eucalyptus proteins involved in early SE is limited.…”

Section: Introductionmentioning

confidence: 99%

Proteome profiles during early stage of somatic embryogenesis of two Eucalyptus species

Chen¹,

Li²,

Chen³

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

Background Somatic embryogenesis (SE) was recognized as an important tool for plants to propagate. However, our knowledge about the proteins involved in early SE including the callus dedifferentiation is still limited, especially in the economic woody tree – Eucalyptus. Results We used the data-independent acquisition mass-spectrometry to study the different proteome profiles of early SE of two Eucalyptus species—E. camaldulensis (high regeneratively potential) and E. grandis x urophylla (low regenerative potential). Initially, 35,207 peptides and 7,077 proteins were identified in the stem and tissue-culture induced callus of the two Eucalyptus species. MSstat identified 2,078 and 2,807 differentially expressed proteins (DEPs) in early SE of E. camaldulensis and E. grandis x urophylla, respectively. They shared 760 upregulated and 420 downregulated proteins, including 4 transcription factors, 31 ribosomal proteins, 1 histone, 3 zinc finger proteins (ZFPs), 16 glutathione transferases, 10 glucosyltransferases, ARF19, WOX8 and PIN1. These proteins might be involved in the early SE of Eucalyptus. By combining the miRNA and RNA-Seq results, some miRNA ~ gene/protein regulatory networks were identified in early SE of Eucalyptus, such as miR160 ~ TPP2, miR164 ~ UXS2, miR169 ~ COX11 and miR535 ~ Eucgr.E01067. Further, we found SERK, WRKY, ZFP and ABC transporter might be related with high SE potential. Conclusions Overall, our study identified proteins involved in the early SE and related to the high regeneration potential of Eucalyptus. It greatly enhanced our understanding of the early SE and the SE capacity of Eucalyptus.

show abstract

Genome-wide identification, evolution analysis of cytochrome P450 monooxygenase multigene family and their expression patterns during the early somatic embryogenesis in Dimocarpus longan Lour.

Cited by 15 publications

References 44 publications

The Involvement of microRNAs in Plant Lignan Biosynthesis—Current View

The Involvement of microRNAs in Plant Lignan Biosynthesis—Current View

Proteome profiles of two Eucalyptus species during the early stage of somatic embryogenesis

Proteome profiles during early stage of somatic embryogenesis of two Eucalyptus species

Contact Info

Product

Resources

About