Genome-Wide Identification, Classification and Expression Analysis of the HSP Gene Superfamily in Tea Plant (Camellia sinensis)

Chen, Jiangfei; Gao, Tong; Wan, Shungang; YongHeng, Zhang; Yang, Jiankun; Yu, Youben; Wang, Weidong

doi:10.3390/ijms19092633

Cited by 65 publications

(60 citation statements)

References 67 publications

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“…The features and functions of HSPs have been widely studied in many plants [ 90 ]. For example, almost all the CsHSP genes of C. sinensis are expressed in one or more organs, and are strongly induced under drought and heat stress [ 91 ]. The expression of loblolly pine ( P. taeda ) HSP genes shows distinctive responses associated with acclimation [ 92 ].…”

Section: Discussionmentioning

confidence: 99%

Characterization and Analysis of the Full-Length Transcriptomes of Multiple Organs in Pseudotaxus chienii (W.C.Cheng) W.C.Cheng

Liu

Wang

et al. 2020

IJMS

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Pseudotaxus chienii, a rare tertiary relict species with economic and ecological value, is a representative of the monotypic genus Pseudotaxus that is endemic to China. P. chienii can adapt well to habitat isolation and ecological heterogeneity under a variety of climate and soil conditions, and is able to survive in harsh environments. However, little is known about the molecular and genetic resources of this long-lived conifer. Herein, we sequenced the transcriptomes of four organs of P. chienii using the PacBio Isoform Sequencing and Illumina RNA Sequencing platforms. Based on the PacBio Iso-Seq data, we obtained 44,896, 58,082, 50,485, and 67,638 full-length unigenes from the root, stem, leaf, and strobilus, respectively, with a mean length of 2692 bp, and a mean N50 length of 3010.75 bp. We then comprehensively annotated these unigenes. The number of organ-specific expressed unigenes ranged from 4393 in leaf to 9124 in strobilus, suggesting their special roles in physiological processes, organ development, and adaptability in the different four organs. A total of 16,562 differentially expressed genes (DEGs) were identified among the four organs and clustered into six subclusters. The gene families related to biotic/abiotic factors, including the TPS, CYP450, and HSP families, were characterized. The expression levels of most DEGs in the phenylpropanoid biosynthesis pathway and plant–pathogen interactions were higher in the root than in the three other organs, suggesting that root constitutes the main organ of defensive compound synthesis and accumulation and has a stronger ability to respond to stress. The sequences were analyzed to predict transcription factors, long non-coding RNAs, and alternative splicing events. The expression levels of most DEGs of C2H2, C3H, bHLH, and bZIP families in the root and stem were higher than those in the leaf and strobilus, indicating that these TFs may play a crucial role in the survival of the root and stem. These results comprise the first comprehensive gene expression profiles obtained for different organs of P. chienii. Our findings will facilitate further studies on the functional genomics, adaptive evolution, and phylogeny of P. chienii, and lay the foundation for the development of conservation strategies for this endangered conifer.

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Section: Discussionmentioning

confidence: 99%

Characterization and Analysis of the Full-Length Transcriptomes of Multiple Organs in Pseudotaxus chienii (W.C.Cheng) W.C.Cheng

Liu

Wang

et al. 2020

IJMS

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“…Белки теплового шока HSPs (heat shock proteins) -вездесущие белки в клетках растений, роль которых сначала сводилась к тепловому стрессу, но теперь стало известно, что они играют важную роль в ответе на разные био тические и абиотические стрессы, помогают поддерживать стабильность клеточных мембран. Установлено, что у арабидопсиса экспрессия HSP90 существенно повышается в условиях жары, холода, засоления, воздействия тяже лых металлов, окислительного стресса (Chen et al, 2018). У чая также было выявлено 47 генов CsHSP из се мейств HSP90, HSP70 и sHSP и отмечена их важная функция в регуляции роста и ответа на засуху и экстремальные температуры.…”

Section: генетические механизмы устойчивости растений чая к холодовомunclassified

Genes underlying cold acclimation in the tea plant (<i>Camellia sinensis</i> (L.) Kuntze)

et al. 2020

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The article reviews the latest studies showing the diversity of genetic mechanisms and gene families underlying the increased cold and frost tolerance of tea and other plant species. It has been shown that cell responses to chilling (0…+15°C) and freezing (< 0°C) are not the same and gene expression under cold stress is genotype-specific. In recent decades, progress has been made in understanding the genetic mechanisms underlying the cold response of plants – ICE1 (inducer of CBF expression 1), CBF (C-repeat-binding factor), COR (cold-regulated genes) pathways and signaling have been discovered. The ICE, CBF and DHN gene groups play a key role in the cold acclimation of the tea plant. The accumulation of CBF transcripts occurs after 15 min of chilling induction, and longer cold stress leads to accumulation of CBF transcripts. It is shown that the transcripts of the CsDHN1, CsDHN2 and CsDHN3 genes accumulate at a higher level in resistant genotypes of tea in comparison with susceptible cultivars during freezing. CBF-independent pathways include genes involved in metabolism and transcription factors such as HSFC1, ZAT12, CZF1, PLD (phospholipase D), WRKY, HD-Zip, CsLEA, LOX, NAC, HSP, which are widely distributed in plants and are involved in the basic mechanisms of tea resistance to cold and frost. The most recent studies show an important role of miRNA in the mechanisms of response to chilling and freezing in tea. The data obtained on different plant species may correlate with the mechanisms of frost tolerance of tea and are the basis for future studies of the signaling pathways of response to cold in the tea plant. The results of the research emphasize the need to further explore the ways in which various genes regulate the tolerance of tea to cold stress to find the molecular markers of frost tolerance.

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“…Множество транскрипционных факторов (DHN, WRKY, HD-Zip, NAC, bHLH и др.) и генов синтеза метаболитов запускаются в ответ на холод (Yue et al, 2015;Wang et al, 2016a, b;Chen et al, 2018;Cui et al, 2018;Shen et al, 2018;Zhu et al, 2018). Об этих и других генах нами был сделан детальный обзор последних публикаций (Самарина и др., 2019).…”

Section: Introductionunclassified

Comparative analysis of gene expression in tea plant (Camellia sinensis (L.) Kuntze) under low-temperature stress

et al. 2020

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Low-temperature stress is one of the main factors limiting the distribution and reducing the yield of many subtropical crops, including the tea crop. Efficient breeding to develop frost-tolerant cultivars requires a reliable set of genetic markers for identifying resistance donors, and that is why it is necessary to reveal the specific genetic response in frost-tolerant genotypes in comparison with frost- susceptible ones. In this work, we performed a comparative analysis of the expression of 18 tea genes (ICE1, CBF1, DHN1, DHN2, DHN3, NAC17, NAC26, NAC30, bHLH7, bHLH43, P5CS, WRKY2, LOX1, LOX6, LOX7, SnRK1.1, SnRK1.2, SnRK1.3) under cold and frost conditions in two tea genotypes, tolerant and susceptible. Low-temperature stress was induced by placing the potted plants in cold chambers and lowering the temperature to 0…+2 °С for 7 days (cold stress), followed by a decrease in temperature to –4…–6 °С for 5 days (frost stress). Relative electrical conductivity of leaf was measured in response to the stress treatments, and a significant difference in the frost tolerance of the two tea genotypes was confirmed. Cold exposure did not lead to a change in the electrical conductivity of leaf tissue. On the other hand, frost treatment resulted in increased REC in both genotypes and to a greater extent in the susceptible genotype. Increased expression of all the genes was shown during cold and frost. The genes that were strongly expressed in the tolerant tea genotype were revealed: ICE1, CBF1, DHN2, NAC17, NAC26, bHLH43, WRKY2, P5CS, LOX6, SnRK1.1, SnRK1.3. These genes can be proposed as markers for the selection of frost-tolerance donors in tea germplasm collections. Additionally, it was shown that the tolerant genotype is characterized by an earlier response to stress at the stage of cold acclimation. The study of the expression of the identified genes in different organs of tea plants and in different exposures to low temperature is relevant for further investigations.

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Genome-Wide Identification, Classification and Expression Analysis of the HSP Gene Superfamily in Tea Plant (Camellia sinensis)

Cited by 65 publications

References 67 publications

Characterization and Analysis of the Full-Length Transcriptomes of Multiple Organs in Pseudotaxus chienii (W.C.Cheng) W.C.Cheng

Characterization and Analysis of the Full-Length Transcriptomes of Multiple Organs in Pseudotaxus chienii (W.C.Cheng) W.C.Cheng

Genes underlying cold acclimation in the tea plant (<i>Camellia sinensis</i> (L.) Kuntze)

Comparative analysis of gene expression in tea plant (Camellia sinensis (L.) Kuntze) under low-temperature stress

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