Genome-wide identification, characterisation and expression analysis of the ALAS gene in the Yesso scallop (Patinopecten yessoensis) with different shell colours

Mao, Junxia; Zhang, Qun; Yuan, Changzi; Zhang, Wenjing; Hu, Liping; Wang, Xubo; Liu, Mingtai; Han, Bing; Ding, Jun; Chang, Yaqing

doi:10.1016/j.gene.2020.144925

Cited by 10 publications

(5 citation statements)

References 42 publications

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“…The Strongylocentrotus purpuratus genome encodes an ALA synthase 62% identical to the mouse ortholog [ 8 , 32 ]. ALA synthase has been cloned from other marine invertebrates such as the bivalve Patinopecten yessoensis , and conserved gene sequences have been identified across taxonomic groups [ 33 ]. It is thus conceivable that despite the lack of the brittle star O. sarsii genomic data, its ALA synthase could be cloned in the future, along with the genes encoding other components of the porphyrin biosynthetic route.…”

Section: Discussionmentioning

confidence: 99%

Chlorin Endogenous to the North Pacific Brittle Star Ophiura sarsii for Photodynamic Therapy Applications in Breast Cancer and Glioblastoma Models

et al. 2022

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Photodynamic therapy (PDT) represents a powerful avenue for anticancer treatment. PDT relies on the use of photosensitizers—compounds accumulating in the tumor and converted from benign to cytotoxic upon targeted photoactivation. We here describe (3S,4S)-14-Ethyl-9-(hydroxymethyl)-4,8,13,18-tetramethyl-20-oxo-3-phorbinepropanoic acid (ETPA) as a major metabolite of the North Pacific brittle stars Ophiura sarsii. As a chlorin, ETPA efficiently produces singlet oxygen upon red-light photoactivation and exerts powerful sub-micromolar phototoxicity against a panel of cancer cell lines in vitro. In a mouse model of glioblastoma, intravenous ETPA injection combined with targeted red laser irradiation induced strong necrotic ablation of the brain tumor. Along with the straightforward ETPA purification protocol and abundance of O. sarsii, these studies pave the way for the development of ETPA as a novel natural product-based photodynamic therapeutic.

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Section: Discussionmentioning

confidence: 99%

Chlorin Endogenous to the North Pacific Brittle Star Ophiura sarsii for Photodynamic Therapy Applications in Breast Cancer and Glioblastoma Models

et al. 2022

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“…Such pigmentation is stable, and its synthesis is not affected by exposure to or lack of UV light. Transcriptomic studies confirm molluscan shell porphyrins are likely synthesized de novo by trochids via the hem pathway (Williams et al 2017), as do transcriptomic and RNAi studies in other molluscs with chemically confirmed shell porphyrins (e.g., Mao et al 2020;Hu et al 2021a,b). Porphyrin shell pigments are thus not derived from the snail's diet and are instead synthesized by enzymes whose presence and expression are genetically controlled.…”

Section: Shell Pigmentation and Patternmentioning

confidence: 80%

“…2017), as do transcriptomic and RNAi studies in other molluscs with chemically confirmed shell porphyrins (e.g., Mao et al. 2020; Hu et al. 2021a,b).…”

Section: Discussionmentioning

confidence: 95%

Evolutionary loss of shell pigmentation, pattern, and eye structure in deep‐sea snails in the dysphotic zone

et al. 2022

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Adaptations to habitats lacking light, such as the reduction or loss of eyes and pigmentation, have fascinated biologists for centuries, yet have rarely been studied in the deep sea, the earth's oldest and largest light‐limited habitat. Here, we investigate the evolutionary loss of shell pigmentation, pattern, and eye structure across a family of deep‐sea gastropods (Solariellidae). We show that within our phylogenetic framework, loss of these traits evolves without reversal, at different rates (faster for shell traits than eye structure), and over different depth ranges. Using a Bayesian approach, we find support for correlated evolution of trait loss with increasing depth within the dysphotic region. A transition to trait loss occurs for pattern and eye structure at 400–500 m and for pigmentation at 600–700 m. We also show that one of the sighted, shallow‐water species, Ilanga navakaensis, which may represent the “best‐case” scenario for vision for the family, likely has poor spatial acuity and contrast sensitivity. We therefore propose that pigmentation and pattern are not used for intraspecific communication but are important for camouflage from visual predators, and that the low‐resolution vision of solariellids is likely to require high light intensity for basic visual tasks, such as detecting predators.

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“…Yesso scallop (Patinopecten yessoensis), a mariculture bivalve, is one of the most important aquaculture species in Asian nations [1]. Scallops were widely distributed along the coastline of Northern Japan, the far east of Russia, the Korean Peninsula, and Bohai Bay of China, which were highly prized for their rapid growth and high nutritional value [2].…”

Section: Introductionmentioning

confidence: 99%

Effects of Ultrasound-Assisted Vacuum Impregnation Antifreeze Protein on the Water-Holding Capacity and Texture Properties of the Yesso Scallop Adductor Muscle during Freeze–Thaw Cycles

Shi

Wang

Zheng

et al. 2022

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The effect of antifreeze protein (AFP) on the water-holding capacity (WHC) and texture properties of the Patinopecten yessoensis adductor muscles during freeze–thaw cycles (FTCs) were evaluated based on three impregnation methods: general impregnation (GI), vacuum impregnation (VI), and ultrasound-assisted VI (US-VI). The WHC, texture properties, and tissue microstructure were all evaluated. Results showed that the WHC and texture properties of adductor muscle were significantly improved in the VI and US-VI groups during FTCs (p < 0.05). The WHC of the adductor muscle in the US-VI group was maximally enhanced in terms of yield (6.63%), centrifugal loss, cooking loss, and T22. The US-VI group of the adductor muscle had the optimal chewiness and springiness compared to others, and the shear force and hardness were most effectively enhanced by VI. The growth and recrystallization of ice crystals in the frozen adductor muscle were significantly inhibited by VI and US-VI. The average cross-sectional area and roundness of ice crystals in the US-VI group were decreased by 61.89% and increased by 22.22% compared with those of the control, respectively. The partial least squares regression (PLSR) model further confirmed that the WHC and texture properties of the adductor muscle were correlated appreciably with the degree of modification of ice crystal morphology through the AFP.

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Genome-wide identification, characterisation and expression analysis of the ALAS gene in the Yesso scallop (Patinopecten yessoensis) with different shell colours

Cited by 10 publications

References 42 publications

Chlorin Endogenous to the North Pacific Brittle Star Ophiura sarsii for Photodynamic Therapy Applications in Breast Cancer and Glioblastoma Models

Chlorin Endogenous to the North Pacific Brittle Star Ophiura sarsii for Photodynamic Therapy Applications in Breast Cancer and Glioblastoma Models

Evolutionary loss of shell pigmentation, pattern, and eye structure in deep‐sea snails in the dysphotic zone

Effects of Ultrasound-Assisted Vacuum Impregnation Antifreeze Protein on the Water-Holding Capacity and Texture Properties of the Yesso Scallop Adductor Muscle during Freeze–Thaw Cycles

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