Genome-Wide Identification and Transcriptome-Based Expression Profile of Cuticular Protein Genes in Antheraea pernyi

Fu, Xin; Chen, Miaomiao; Xia, Run-Xi; Li, Xinyu; Qun, LI; Li, Yuping; Cao, Huiying; Liu, Yan-Qun

doi:10.3390/ijms24086991

Cited by 5 publications

(4 citation statements)

References 63 publications

(146 reference statements)

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“…In the epidermis of Lepidoptera, CPH genes often exhibit high expression levels. For example, BmorCPH24 has the highest expression level in the silkworm larval epidermis ( Fu et al, 2023 ). The CPG protein is rich in glycine.…”

Section: Discussionmentioning

confidence: 99%

The BTB-ZF gene Bm-mamo regulates pigmentation in silkworm caterpillars

Wu,

Tong,

Peng

et al. 2024

eLife

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Color pattern of insects is one of the most dazzling adaptive evolutionary phenotypes. However, the molecular regulation of this color pattern is not clear. In this paper, we found a transcription factor, Bm-mamo, is responsible for bd (black dilute) allele mutants in silkworm. It belongs to BTB zinc finger family, and is ortholog to mamo of Drosophila melanogaster, which It is found that this gene has conservative function in gamete production, and evolved a pleiotropic function in regulation of color patterns in caterpillar. We found that the Bm-mamo can comprehensively regulate the expression of related pigment synthesis and cuticular protein genes to form color patterns. This suggests that the deposition of pigment particles in caterpillars’ epidermis requires not only the spatiotemporal expression of pigment synthesis genes, but also the correct expression of related cuticular protein genes. This study provides a new data for the setting of color patterns.

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Section: Discussionmentioning

confidence: 99%

The BTB-ZF gene Bm-mamo regulates pigmentation in silkworm caterpillars

Wu,

Tong,

Peng

et al. 2024

eLife

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“…In the epidermis of Lepidoptera, CPH genes often exhibit high expression levels. For example, BmorCPH24 has the highest expression level in the silkworm larval epidermis( 72 ). The CPG protein is rich in glycine.…”

Section: Discussionmentioning

confidence: 99%

The BTB-ZF geneBm-mamoregulates pigmentation in silkworm caterpillars

Tong

Peng

et al. 2023

Preprint

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Color pattern of insects is one of the most dazzling adaptive evolutionary phenotypes. However, the molecular regulation of this color pattern is not clear. In this paper, we found a transcription factor, Bm-mamo, is responsible for bd (black dilute) allele mutants in silkworm. It belongs to BTB zinc finger family, and is ortholog to mamo of Drosophila melanogaster, which It is found that this gene has conservative function in gamete production, and evolved a pleiotropic function in regulation of color patterns in caterpillar. We found that the Bm-mamo can comprehensively regulate the expression of related pigment synthesis and cuticular protein genes to form color patterns. This suggests that the deposition of pigment particles in caterpillars' epidermis requires not only the spatiotemporal expression of pigment synthesis genes, but also the correct expression of related cuticular protein genes. This study provides a new data for the setting of color patterns.

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“…R&R-type cuticular proteins also have a chitin-binding domain, which is typically expressed in the epidermis but not found in the peritrophic matrix [67]. Based on transcriptome-based expression analyses of the midgut [68], we assume these R&R-type proteins originate from midgut epithelial cells.…”

Section: Chitin-related Proteins and Nutritional Proteinsmentioning

confidence: 99%

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

Wang,

Yang,

Tian

et al. 2023

Insects

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The silkworm Bombyx mori, a lepidopteran insect, possesses an 8–10-day pupal stage, during which significant changes occur in the midgut, where it first condenses into the yellow body, and then undergoes decomposition. To gain insights into this transformation process, proteomics was performed on Bombyx mori midgut contents on day 2 and day 7 after pupation. The results revealed the identification of 771 proteins with more than one unique peptide. An analysis using AgriGO demonstrated that these proteins were predominantly associated with catalytic activity. Among the identified proteins, a considerable number were found to be involved in carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleic acid degradation, and energy support. Additionally, variations in the levels of certain proteases were observed between the midgut contents on day 2 and day 7 after pupation. An in-depth analysis of the two-dimensional electrophoresis of the midgut contents on day 7 after pupation led to the identification of twelve protein spots with potential gelatinolytic activity. Among these, six proteases were identified through mass spectrometry, including the p37k protease, vitellin-degrading protease, chymotrypsin-2, etc. These proteases may be responsible for the digestion of the yellow body during the later stages of pupal development.

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Genome-Wide Identification and Transcriptome-Based Expression Profile of Cuticular Protein Genes in Antheraea pernyi

Cited by 5 publications

References 63 publications

The BTB-ZF gene Bm-mamo regulates pigmentation in silkworm caterpillars

The BTB-ZF gene Bm-mamo regulates pigmentation in silkworm caterpillars

The BTB-ZF geneBm-mamoregulates pigmentation in silkworm caterpillars

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

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