Genome-wide identification and functional analysis of the peach (P. persica) laccase gene family reveal members potentially involved in endocarp lignification

Qui, Keli; Zhou, Hui; Pan, Haifa; Yu, Shanlin; Yu, Hong; Xie, Qingmei; Chen, Hongli; Cai, Yongping; Zhang, Jinyun; He, Jinling

doi:10.1007/s00468-022-02296-y

Cited by 5 publications

(15 citation statements)

References 61 publications

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“…The most abundant laccases were observed in Swetha (n = 56), followed by Zhongzhi13 (n = 48), Goenbaek (n = 45), Baizhima (n = 42), Yuzhi11 (n = 40), and Mishuozhima (n = 35). Similar laccases counts were observed in land plants Prunus persica (n = 48) [ 82 ], Panicum virgatum (n = 49) [ 83 ], and Solanum melongena (n = 42) [ 84 ]; a lower number of laccase genes was reported in A. thaliana (n = 17) [ 85 ].…”

Section: Resultssupporting

confidence: 70%

Characterization of Peroxidase and Laccase Gene Families and In Silico Identification of Potential Genes Involved in Upstream Steps of Lignan Formation in Sesame

Zoclanclounon

Rostás

Chung

et al. 2022

Life

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Peroxidases and laccases are oxidative enzymes involved in physiological processes in plants, covering responses to biotic and abiotic stress as well as biosynthesis of health-promoting specialized metabolites. Although they are thought to be involved in the biosynthesis of (+)-pinoresinol, a comprehensive investigation of this class of enzymes has not yet been conducted in the emerging oil crop sesame and no information is available regarding the potential (+)-pinoresinol synthase genes in this crop. In the present study, we conducted a pan-genome-wide identification of peroxidase and laccase genes coupled with transcriptome profiling of diverse sesame varieties. A total of 83 and 48 genes have been identified as coding for sesame peroxidase and laccase genes, respectively. Based on their protein domain and Arabidopsis thaliana genes used as baits, the genes were classified into nine and seven groups of peroxidase and laccase genes, respectively. The expression of the genes was evaluated using dynamic transcriptome sequencing data from six sesame varieties, including one elite cultivar, white vs black seed varieties, and high vs low oil content varieties. Two peroxidase genes (SiPOD52 and SiPOD63) and two laccase genes (SiLAC1 and SiLAC39), well conserved within the sesame pan-genome and exhibiting consistent expression patterns within sesame varieties matching the kinetic of (+)-pinoresinol accumulation in seeds, were identified as potential (+)-pinoresinol synthase genes. Cis-acting elements of the candidate genes revealed their potential involvement in development, hormonal signaling, and response to light and other abiotic triggers. Transcription factor enrichment analysis of promoter regions showed the predominance of MYB binding sequences. The findings from this study pave the way for lignans-oriented engineering of sesame with wide potential applications in food, health and medicinal domains.

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Section: Resultssupporting

confidence: 70%

Characterization of Peroxidase and Laccase Gene Families and In Silico Identification of Potential Genes Involved in Upstream Steps of Lignan Formation in Sesame

Zoclanclounon

Rostás

Chung

et al. 2022

Life

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“…This cycle of competition for nutrient uptake among fruit tissues and seeds highlights the presence of cyclic activities [ 29 , 30 , 31 ]. The endosperm expands fast during nucellus absorption during stage II when the endocarp is lignifying, and metabolites stored in the endosperm subsequently stimulate embryo growth [ 4 , 31 , 32 ].…”

Section: Molecular Mechanisms Of Endocarp Lignificationmentioning

confidence: 99%

“…Based on phylogenetic, sequence, and expression profiling analyses, Qui et al [ 4 ] stated that peach laccase genes ( PpLAC7 , PpLAC19 , PpLAC20 , PpLAC21 , PpLAC27 , PpLAC 28, and PpLAC30 ) may be related to lignin biosynthesis and endocarp hardness in peach fruit. Similarly, PpLAC20 and PpLAC30 are most likely important members involved in peach lignin biosynthesis [ 4 ]. A peach MYB TF, PpMYB63, a homolog of AtMYB58 and AtMYB63, can activate the PpLAC20 and PpLAC30 promoters.…”

Section: Molecular Mechanisms Of Endocarp Lignificationmentioning

confidence: 99%

“…A peach MYB TF, PpMYB63, a homolog of AtMYB58 and AtMYB63, can activate the PpLAC20 and PpLAC30 promoters. Thus, PpLAC20 and PpLAC30 are candidates involved in peach lignin biosynthesis, hardening the peach endocarp [ 4 ].…”

Section: Molecular Mechanisms Of Endocarp Lignificationmentioning

confidence: 99%

“…Almonds and certain apricots, whose seeds are consumed, are exceptional cases. These fruits include apricots, cherries, and numerous species of commercially important plums, peaches, and nectarines [ 2 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Mechanism of Stone (Hardened Endocarp) Formation in Fruits: An Attempt toward Pitless Fruits, and Its Advantages and Disadvantages

Khan

Noor

Jia

et al. 2022

Genes

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Stone (hardened endocarp) has a very important role in the continuity of plant life. Nature has gifted plants with various seed protection and dispersal strategies. Stone-fruit-bearing species have evolved a unique adaptation in which the seed is encased in an extremely hard wood-like shell called the stone. The lignification of the fruit endocarp layer produces the stone, a feature that separates drupes from other plants. Stone cells emerge from parenchyma cells after programmed cell death and the deposition of cellulose and lignin in the secondary cell wall. Generally, the deposition of lignin in primary cell walls is followed by secondary thickening of cell walls to form stone cells. This review article describes the molecular mechanisms and factors that influence the production of stone in the fruit. This is the first review article that describes the molecular mechanisms regulating stone (harden endocarp) formation in fruits. This article will help breeders understand the molecular and genetic basis for the stone formation in fruit, and this could lead to new and innovative directions to breed stoneless fruit cultivars in the future.

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Genome-Wide Identification of Laccase Gene Family from Punica granatum and Functional Analysis towards Potential Involvement in Lignin Biosynthesis

et al. 2023

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Laccase (LAC) is the key enzyme responsible for lignin biosynthesis. Here, 57 PgLACs from pomegranate were identified and distributed on eight chromosomes and one unplaced scaffold. They were divided into six groups containing three typical Cu-oxidase domains. Totally, 51 cis-acting elements in the promoter region of PgLACs are involved in response to ABA, GA, light, stress, etc., indicating diverse functions of PgLACs. The expression profiles of 13 PgLACs during the seed development stage showed that most PgLACs expressed at a higher level earlier than at the later seed development stage in two pomegranate cultivars except PgLAC4. Also, PgLAC1/6/7/16 expressed at a significantly higher level in soft-seed ‘Tunisia’; on the contrary, PgLAC37 and PgLAC50 with a significantly higher expression in hard-seed ‘Taishanhong’. Combined with their distinguishing cis-acting elements, it was concluded that PgLAC1/6/7 may respond to GA via TATC-box and GARE-motif, and PgLAC16 repressed the promotor activity of embryo mid-maturation genes via RY-element so as to contribute to softer seed formation, whereas PgLAC37/50 may participate in seed formation and accelerate seed maturity via ABRE and G-box elements. Collectively, the dramatic gene expressions of PgLAC1/6/7/16/37/50 will provide valuable information to explore the formation of soft- and hard-seed in pomegranate.

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Genome-wide identification and functional analysis of the peach (P. persica) laccase gene family reveal members potentially involved in endocarp lignification

Cited by 5 publications

References 61 publications

Characterization of Peroxidase and Laccase Gene Families and In Silico Identification of Potential Genes Involved in Upstream Steps of Lignan Formation in Sesame

Characterization of Peroxidase and Laccase Gene Families and In Silico Identification of Potential Genes Involved in Upstream Steps of Lignan Formation in Sesame

Mechanism of Stone (Hardened Endocarp) Formation in Fruits: An Attempt toward Pitless Fruits, and Its Advantages and Disadvantages

Genome-Wide Identification of Laccase Gene Family from Punica granatum and Functional Analysis towards Potential Involvement in Lignin Biosynthesis

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