Genome-Wide Identification and Expression Profiling of the BZR Transcription Factor Gene Family in Nicotiana benthamiana

Abstract

Brassinazole-resistant (BZR) family genes encode plant-specific transcription factors (TFs), play essential roles in the regulation of plant growth and development, and have multiple stress-resistance functions. Nicotiana benthamiana is a model plant widely used in basic research. However, members of the BZR family in N. benthamiana have not been identified, and little is known about their function in abiotic stress. In this study, a total of 14 BZR members were identified in the N. benthamiana genome, which could be divided into four groups according to a phylogenetic tree. NbBZRs have similar exon-intron structures and conserved motifs, and may be regulated by cis-acting elements such as STRE, TCA, and ARE, etc. Organ-specific expression analysis showed that NbBZR members have different and diverse expression patterns in different tissues, and most of the members are expressed in roots, stems, and leaves. The analysis of the expression patterns in response to different abiotic stresses showed that all the tested NbBZR members showed a significant down-regulation after drought treatment. Many NbBZR genes also responded in various ways to cold, heat and salt stress treatments. The results imply that NbBZRs have multiple functions related to stress resistance.

Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L.

“…The newly released reference genome of bread wheat ( Triticum aestivum ) used in this study was downloaded from the Ensemble Plants database ( , accessed on 29 December 2021). TaOSCA was identified through two rounds of BLASTP referring to Wu et al and Chen et al [ 34 , 35 ]. Firstly, all A. thaliana OSCA sequences were used to search for possible TaOSCA sequences through TBtools [ 36 ].…”

Genome-wide analysis of the BoBZR1 family genes and transcriptome analysis in Brassica oleracea

“…Rich in essential components, white cabbage contributes to attaining a harmonious vegetable crop and is recognized for its potential health bene ts, including antioxidant properties [24][25][26]. To date, BZR1 gene analysis has been reported in B. rapa [27], Pyrus bretschneideri [28], Nicotiana benthamiana [29], potato [30], maize [31] and Arabidopsis [32]. Yet, our knowledge regarding the evolutionary history and expression pro les of Brassinazole-resistant proteins (BZR1) in B. oleracea is currently constrained.…”

Identification and Characterization of the BZR Transcription Factor Genes Family in Potato (Solanum tuberosum L.) and Their Expression Profiles in Response to Abiotic Stresses

“…Plant hormones are major endogenous signaling molecules that can respond rapidly to environmental stimuli [1]. Brassinosteroids (BRs), a class of plant steroid hormones, play an important role in regulating plant growth and development, along with biotic and abiotic stress responses [2,3]. BRs fully stimulate the anti-stress potential of plants, alleviating damage caused when encountering various adverse external environmental cues through internal regulation [4].…”

“…BZR proteins contain a nuclear localization sequence (NLS) at the N-terminal, a highly conserved DNA binding domain, a phosphorylation domain (which can be phosphorylated by BRASSINOSTEROID INSENSITIVE2-BIN2), a PEST sequence and a C-terminal domain [10]. BZR1 and Brassinosteroid insensitive 1-ethyl methanesulfonate-suppressor 1 (BES1) are two downstream TFs belonging to the BZR TF family [2]. BZR1 and BES1 share 88% amino acid sequence similarity, and the sequence consistency of the DNA binding domain is 97% [11].…”