Genome-wide identification and expression profiling of the copper transporter gene family in Populus trichocarpa

Zhang, Haizhen; Yang, Jingli; Wang, Weida; Li, Dandan; Hu, Xiaoqing; Wang, Han; Wei, Ming; Liu, Quangang; Wang, Zhanchao; Li, Chenghao

doi:10.1016/j.plaphy.2015.10.035

Cited by 22 publications

(28 citation statements)

References 41 publications

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“…) and HSF (Zhang et al . ). As outlined earlier, the whole‐genome sequence information, now available for the salt‐resistant P. euphratica (Ma et al .…”

Section: Improvement Of Tree Salt Tolerance By Genetic Engineeringmentioning

confidence: 97%

On the salty side of life: molecular, physiological and anatomical adaptation and acclimation of trees to extreme habitats

Polle

Chen

2014

Plant Cell & Environment

131

114

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Saline and sodic soils that cannot be used for agriculture occur worldwide. Cultivating stress-tolerant trees to obtain biomass from salinized areas has been suggested. Various tree species of economic importance for fruit, fibre and timber production exhibit high salinity tolerance. Little is known about the mechanisms enabling tree crops to cope with high salinity for extended periods. Here, the molecular, physiological and anatomical adjustments underlying salt tolerance in glycophytic and halophytic model tree species, such as Populus euphratica in terrestrial habitats, and mangrove species along coastlines are reviewed. Key mechanisms that have been identified as mediating salt tolerance are discussed at scales from the genetic to the morphological level, including leaf succulence and structural adjustments of wood anatomy. The genetic and transcriptomic bases for physiological salt acclimation are salt sensing and signalling networks that activate target genes; the target genes keep reactive oxygen species under control, maintain the ion balance and restore water status. Evolutionary adaptation includes gene duplication in these pathways. Strategies for and limitations to tree improvement, particularly transgenic approaches for increasing salt tolerance by transforming trees with single and multiple candidate genes, are discussed.

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“…) and HSF (Zhang et al . ). As outlined earlier, the whole‐genome sequence information, now available for the salt‐resistant P. euphratica (Ma et al .…”

Section: Improvement Of Tree Salt Tolerance By Genetic Engineeringmentioning

confidence: 97%

On the salty side of life: molecular, physiological and anatomical adaptation and acclimation of trees to extreme habitats

Polle

Chen

2014

Plant Cell & Environment

131

114

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“…4 ). The expansion of the CCH gene family in poplar, compared with Arabidopsis and rice, may be related to the stronger growth and adaptability of woody plants than herbaceous plants on the land, and also reflected the specialized roles played by these genes in copper transport and copper homeostasis regulation ( Tuskan et al, 2006 ; Zhang et al, 2015 ). In addition, the CCH genes of Populus and Arabidopsis had fewer than three introns.…”

Section: Discussionmentioning

confidence: 99%

“…The genome sequences of Populus trichocarpa (Torr. & Gray) was published in 2006, and from then on, Populus has been selected as a model tree system for genetic research ( Taylor, 2002 ; Tuskan et al, 2006 ; Zhang et al, 2015 ). Populus species have been used as heavy metal phytoremediation plants as they are adaptable to grow on contaminated areas and able to accumulate metals than short-lived plants ( Di Loardo et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression profile analysis of CCH gene family in Populus

Zhang

Gao

Tang

et al. 2017

PeerJ

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Copper plays key roles in plant physiological activities. To maintain copper cellular homeostasis, copper chaperones have important functions in binding and transporting copper to target proteins. Detailed characterization and function analysis of a copper chaperone, CCH, is presently limited to Arabidopsis. This study reports the identification of 21 genes encoding putative CCH proteins in Populus trichocarpa. Besides sharing the conserved metal-binding motif MXCXXC and forming a βαββαβ secondary structure at the N-terminal, all the PtCCHs possessed the plant-exclusive extended C-terminal. Based on their gene structure, conserved motifs, and phylogenetic analysis, the PtCCHs were divided into three subgroups. Our analysis indicated that whole-genome duplication and tandem duplication events likely contributed to expansion of the CCH gene family in Populus. Tissue-specific data from PlantGenIE revealed that PtCCH genes had broad expression patterns in different tissues. Quantitative real-time RT-PCR (qRT-PCR) analysis revealed that PnCCH genes of P. simonii × P. nigra also had different tissue-specific expression traits, as well as different inducible-expression patterns in response to copper stresses (excessive and deficiency). In summary, our study of CCH genes in the Populus genome provides a comprehensive analysis of this gene family, and lays an important foundation for further investigation of their roles in copper homeostasis of poplar.

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“…Total RNA was extracted using the cetyltrimethylammonium bromide method (Zhang et al, 2015), and reverse-transcription was performed using the PrimeScript RT First Strand cDNA Synthesis Kit (Toyobo). First-strand cDNA synthesized from 0.5 mg of purified RNA was reverse-transcribed using a Reverse Transcriptase Kit (TaKaRa).…”

Section: Rna Extraction and Rt-qpcr Analysismentioning

confidence: 99%

PuHSFA4a Enhances Tolerance To Excess Zinc by Regulating Reactive Oxygen Species Production and Root Development in Populus

Zhang

Yang

et al. 2019

Plant Physiol.

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Zinc (Zn) is an essential micronutrient but in excess is highly toxic to plants. Plants regulate Zn homeostasis and withstand excess Zn through various pathways; these pathways are generally tightly regulated by a specific set of genes. However, the transcription factors involved in excess Zn tolerance have yet to be identified. Here, we characterized a Populus ussuriensis heat shock transcription factor A4a (PuHSFA4a) that acts as a positive regulator of excess Zn tolerance in P. ussuriensis. We used overexpression (PuHSFA4a-OE) and chimeric dominant repressor (PuHSFA4a-SRDX) lines to identify the targets of PuHSFA4a. PuHSFA4a transcription is specifically induced in roots by high Zn. Overexpression of PuHSFA4a conferred excess Zn tolerance and a dominant repressor version of PuHSFA4a increased excess Zn sensitivity in P. ussuriensis by regulating the antioxidant system in roots. PuHSFA4a coordinately activates genes related to abiotic stress responses and root development and directly binds to the promoter regions of glutathione-S-transferase U17 (PuGSTU17) and phospholipase A 2 (PuPLA 2). PuGSTU17 overexpression significantly increased GST activity and reduced reactive oxygen species levels in roots while PuGSTU17-RNA interference lines exhibited the opposite phenotype. Furthermore, PuPLA 2 overexpression promoted root growth under high Zn stress. Taken together, we provide evidence that PuHSFA4a coordinately activates the antioxidant system and root development-related genes and directly targets PuGSTU17 and PuPLA, thereby promoting excess Zn tolerance in P. ussuriensis roots.

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Genome-wide identification and expression profiling of the copper transporter gene family in Populus trichocarpa

Cited by 22 publications

References 41 publications

On the salty side of life: molecular, physiological and anatomical adaptation and acclimation of trees to extreme habitats

On the salty side of life: molecular, physiological and anatomical adaptation and acclimation of trees to extreme habitats

Genome-wide identification and expression profile analysis of CCH gene family in Populus

PuHSFA4a Enhances Tolerance To Excess Zinc by Regulating Reactive Oxygen Species Production and Root Development in Populus

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