Genome-wide identification and expression profiles of sex-related gene families in the Pacific abalone Haliotis discus hannai

Zhang, Qian; Huang, Jianfang; Fu, Yangtao; Chen, Jianming; Wang, Wei

doi:10.1016/j.cbd.2024.101205

Cited by 3 publications

(2 citation statements)

References 75 publications

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“…In the present study, SoxF was ubiquitously expressed in early developmental stage and adult tissues. A similar result was found in the Pacific abalone Haliotis discus hannai [ 50 ]. These results suggest that the SoxF gene has diverse functions in aquatic invertebrates.…”

Section: Discussionsupporting

confidence: 86%

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Identification and expression analysis of Sox family genes in echinoderms

Li,

Cao,

Liu

et al. 2024

BMC Genomics

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The Sox gene family, a collection of transcription factors widely distributed throughout the animal kingdom, plays a crucial role in numerous developmental processes. Echinoderms occupy a pivotal position in many research fields, such as neuroscience, sex determination and differentiation, and embryonic development. However, to date, no comprehensive study has been conducted to characterize and analyze Sox genes in echinoderms. In the present study, the evolution and expression of Sox family genes across 11 echinoderms were analyzed using bioinformatics methods. The results revealed a total of 70 Sox genes, with counts ranging from 5 to 8 across different echinoderms. Phylogenetic analysis revealed that the identified Sox genes could be categorized into seven distinct classes: the SoxB1 class, SoxB2 class, SoxC class, SoxD class, SoxE class, SoxF class and SoxH class. Notably, the SoxB1, SoxB2, and SoxF genes were ubiquitously present in all the echinoderms studied, which suggests that these genes may be conserved in echinoderms. The spatiotemporal expression patterns observed for Sox genes in the three echinoderms indicated that various Sox members perform distinct functional roles. Notably, SoxB1 is likely involved in echinoderm ovary development, while SoxH may play a crucial role in testis development in starfish and sea cucumber. In general, the present investigation provides a molecular foundation for exploring the Sox gene in echinoderms, providing a valuable resource for future phylogenetic and genomic studies.

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Section: Discussionsupporting

confidence: 86%

“…SoxH genes, which were previously thought to be mammalian specific, have been identified in several invertebrates, including ascidians [ 28 ], oysters [ 48 ], clams [ 49 ], scallops [ 9 ], and abalone [ 50 ]. SoxH usually has male-biased expression in these mollusks.…”

Section: Discussionmentioning

confidence: 99%

Identification and expression analysis of Sox family genes in echinoderms

Li,

Cao,

Liu

et al. 2024

BMC Genomics

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Japanese Medaka Dmrt3a is Involved in Male Fertility

Chen,

Deng,

Huang

et al. 2024

Preprint

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Medaka (Oryzias latipes) Dmrt3a Is Involved in Male Fertility

Deng,

Huang,

Liang

et al. 2024

Animals

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Research across various species has demonstrated that the doublesex and mab-3-related transcription factor 3 (dmrt3) plays pivotal roles in testis development. However, the precise molecular mechanisms of dmrt3 remain unclear. In this study, we investigated the role of dmrt3 (dmrt3a) in testis development using the model organism medaka (Oryzias latipes). SqRT-PCR and ISH analyses revealed that dmrt3a is predominantly expressed in the testis, especially in the spermatid and spermatozoon. Using CRISPR/Cas9, we generated two dmrt3a homozygous mutants (-8 bp and -11 bp), which exhibited significantly reduced fertilization rates and embryo production. Additionally, the number of germ cells and sperm motility were markedly decreased in the dmrt3a mutants, manifesting as the symptoms of asthenozoospermia and oligozoospermia. Interestingly, RNA-Seq analysis showed that the deficiency of dmrt3a could lead to a significant downregulation of numerous genes related to gonadal development and severe disruptions in mitochondrial function. These results suggested that dmrt3a is essential for spermatogenesis and spermatozoa energy production. This paper provides new insights and perspectives for further exploring the molecular mechanisms underlying spermatogenesis and addressing male reproductive issues.

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Genome-wide identification and expression profiles of sex-related gene families in the Pacific abalone Haliotis discus hannai

Cited by 3 publications

References 75 publications

Identification and expression analysis of Sox family genes in echinoderms

Identification and expression analysis of Sox family genes in echinoderms

Japanese Medaka Dmrt3a is Involved in Male Fertility

Medaka (Oryzias latipes) Dmrt3a Is Involved in Male Fertility

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