Genome-wide identification and expression pattern of alkaline/neutral invertase gene family inDendrobium catenatum

Abstract

Alkaline/neutral invertase (NI) proteins irreversibly break down sucrose into glucose and fructose and play a vital role in carbohydrate and plant growth and development. Currently, a comprehensive understanding of NI genes in Dendrobium catenatum is lacking. After genome-wide identification and prediction, nine DcNI genes were identified and named DcNI1-DcNI9. Through comprehensive analysis of gene structure, amino acid sequence, conserved motif and phylogenetic tree, it was found that the DcNI gene family has a typical conserved region and its amino acid sequence is similar to other plant NI genes. However, DcNI3 lost amino acid sequences at both the N-terminus and C-terminus, while DcNI8 lost amino acids at the C-terminus. Phylogenetic analysis revealed that the DcNI family could be divided into α and β groups. It reveals conservation and divergence of DcNIs. Predictive analysis of cis-acting elements in the promoter region reveals the potential response of DcNI protein to hormones and environmental stress and suggests it to be widely involved in growth and development. DcNIs were differentially expressed in different tissues such as roots, stems and leaves. In particular, DcNI4 was highly expressed in the stem. They also had a differential expression response under drought, cold, P1 fungus infection and jasmonic acid (JA) treatment. To the best of our knowledge, this is the first genome-wide analysis of the DcNI gene family in D. catenatum. This study will provide important and primary information for the functional study of the DcNI gene family and the polysaccharide synthesis pathway in D. catenatum.

Amelioration of cold-induced sweetening in potato by RNAi mediated silencing of StUGPase encoding UDP-glucose pyrophosphorylase

“…Numerous efforts have been made to manipulate the expression of genes encoding the key regulatory enzymes, transcriptional factors, and other regulatory molecules that are directly or indirectly involved in CIS with variable degrees of success in reducing RS accumulation during CIS (Schwimmer et al, 1961;Sowokinos, 2001a;Geigenberger et al, 2004;Liu et al, 2010;Brummell et al, 2011;Liu et al, 2013;McKenzie et al, 2013;Zhang et al, 2013;Shivalingamurthy et al, 2018;Shi et al, 2021, Shi et al, 2022. Vacuolar acid invertase (vaINV), catalyzing the penultimate step, has been identified as one of the major factors involved in CIS (Zrenner et al, 1996;Matsuura-Endo et al, 2004;McKenzie et al, 2005;Liu et al, 2021). A few studies reported considerable improvement of CIS by down regulation of the StvaINV expression (Bhaskar et al, 2010;Wiberley-Bradford et al, 2014;Zhu et al, 2014;Hameed et al, 2018).…”