Genome‑wide identification and expression analysis of the ASMT gene family reveals their role in abiotic stress tolerance in apple

Wang, Hongtao; Song, Chunhui; Fang, Sen; Wang, Zhengyang; Song, Shangwei; Jiao, Jian; Wang, Miaomiao; Zheng, Xianbo; Bai, Tuanhui

doi:10.1016/j.scienta.2021.110683

Cited by 19 publications

(18 citation statements)

References 43 publications

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“…In soybeans, transgenic expression of GmASMT3/44/17/7 led to a significant increase in heat and drought resistance 68 . Apple ASMT ( MdASMT ) had a close genetic distance with rice ASMT ( OsASMT ), 69 which revealed that MdASMT1/11/14/9 exhibited similar functions with OsASMT for inducing drought, salt, and osmotic tolerance 70–74 . Similarly, overexpression of VvASMT1 in grape significantly alleviated salt and osmotic damage 75 .…”

Section: Melatonin Biosynthesismentioning

confidence: 97%

“…For vegetative growth, ASMT negatively regulated seed germination, and a decrease in germination rate was observed in ASMT overexpressing Arabidopsis 115 . MdASMT11/14 could regulate apple root development under various stress 70 . The high expression of OsASMT1/2 in stems, as well as the absence of any detectable level of ASMT3 in any plant organ, implied that OsASMT isoforms played the different role in stem development 67 .…”

Section: Melatonin Biosynthesismentioning

confidence: 99%

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Genetic and evolutionary dissection of melatonin response signaling facilitates the regulation of plant growth and stress responses

Gao

Chen

et al. 2023

Journal of Pineal Research

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The expansion of gene families during evolution could generate functional diversity among their members to regulate plant growth and development. Melatonin, a phylogenetically ancient molecule, is vital for many aspects of a plant's life. Understanding the functional diversity of the molecular players involved in melatonin biosynthesis, signaling, and metabolism will facilitate the regulation of plant phenotypes. However, the molecular mechanism of melatonin response signaling elements in regulating this network still has many challenges. Here, we provide an in‐depth analysis of the functional diversity and evolution of molecular components in melatonin signaling pathway. Genetic analysis of multiple mutants in plant species will shed light on the role of gene families in melatonin regulatory pathways. Phylogenetic analysis of these genes was performed, which will facilitate the identification of melatonin‐related genes for future study. Based on the abovementioned signal networks, the mechanism of these genes was summarized to provide reference for studying the regulatory mechanism of melatonin in plant phenotypes. We hope that this work will facilitate melatonin research in higher plants and finely tuned spatio‐temporal regulation of melatonin signaling.

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Section: Melatonin Biosynthesismentioning

confidence: 97%

Section: Melatonin Biosynthesismentioning

confidence: 99%

Genetic and evolutionary dissection of melatonin response signaling facilitates the regulation of plant growth and stress responses

Gao

Chen

et al. 2023

Journal of Pineal Research

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“…They noted that ASMT genes were significantly upregulated under cold, drought and heat stress. The expression of these genes appreciably increased MT biosynthesis, which increased the plant tolerance to cold, drought, and heat stresses ( Wang et al, 2022 ).…”

Section: Engineering Melatonin Biosynthesis Improves Cold Tolerancementioning

confidence: 99%

Melatonin Induced Cold Tolerance in Plants: Physiological and Molecular Responses

et al. 2022

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Cold stress is one of the most limiting factors for plant growth and development. Cold stress adversely affects plant physiology, molecular and biochemical processes by determining oxidative stress, poor nutrient and water uptake, disorganization of cellular membranes and reduced photosynthetic efficiency. Therefore, to recover impaired plant functions under cold stress, the application of bio-stimulants can be considered a suitable approach. Melatonin (MT) is a critical bio-stimulant that has often shown to enhance plant performance under cold stress. Melatonin application improved plant growth and tolerance to cold stress by maintaining membrane integrity, plant water content, stomatal opening, photosynthetic efficiency, nutrient and water uptake, redox homeostasis, accumulation of osmolytes, hormones and secondary metabolites, and the scavenging of reactive oxygen species (ROS) through improved antioxidant activities and increase in expression of stress-responsive genes. Thus, it is essential to understand the mechanisms of MT induced cold tolerance and identify the diverse research gaps necessitating to be addressed in future research programs. This review discusses MT involvement in the control of various physiological and molecular responses for inducing cold tolerance. We also shed light on engineering MT biosynthesis for improving the cold tolerance in plants. Moreover, we highlighted areas where future research is needed to make MT a vital antioxidant conferring cold tolerance to plants.

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“…ASMT genes have been identified in more than 10 species, including Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), tomato (Solanum lycopersicum), pepper (Capsicum annuum), apple (Malus domestica), and wheat (Triticum aestivum; Liu et al, 2017;Pan et al, 2019;Zhan et al, 2019;Bhowal et al, 2021;Wang et al, 2022). Functions of ASMT in plant biotic and abiotic stress responses have been extensively characterized.…”

Section: Introductionmentioning

confidence: 99%

“…Elevated expression of MdASMT1 increased apple stomatal performance and water content under drought stress. MdASMT11 and MdASMT14 are assumed to play essential roles in the response of apple rootstocks to abiotic stresses (Wang et al, 2022). ASMT also participated in multiple growth and developmental dynamics in plants.…”

Section: Introductionmentioning

confidence: 99%

Walnut N-Acetylserotonin Methyltransferase Gene Family Genome-Wide Identification and Diverse Functions Characterization During Flower Bud Development

Zhao

et al. 2022

Front. Plant Sci.

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Melatonin widely mediates multiple developmental dynamics in plants as a vital growth stimulator, stress protector, and developmental regulator. N-acetylserotonin methyltransferase (ASMT) is the key enzyme that catalyzes the final step of melatonin biosynthesis in plants and plays an essential role in the plant melatonin regulatory network. Studies of ASMT have contributed to understanding the mechanism of melatonin biosynthesis in plants. However, AMST gene is currently uncharacterized in most plants. In this study, we characterized the JrASMT gene family using bioinformatics in a melatonin-rich plant, walnut. Phylogenetic, gene structure, conserved motifs, promoter elements, interacting proteins and miRNA analyses were also performed. The expansion and differentiation of the ASMT family occurred before the onset of the plant terrestrialization. ASMT genes were more differentiated in dicotyledonous plants. Forty-six ASMT genes were distributed in clusters on 10 chromosomes of walnut. Four JrASMT genes had homologous relationships both within walnut and between species. Cis-regulatory elements showed that JrASMT was mainly induced by light and hormones, and targeted cleavage of miRNA172 and miR399 may be an important pathway to suppress JrASMT expression. Transcriptome data showed that 13 JrASMT were differentially expressed at different periods of walnut bud development. WGCNA showed that JrASMT1/10/13/23 were coexpressed with genes regulating cell fate and epigenetic modifications during early physiological differentiation of walnut female flower buds. JrASMT12/28/37/40 were highly expressed during morphological differentiation of flower buds, associated with altered stress capacity of walnut flower buds, and predicted to be involved in the regulatory network of abscisic acid, salicylic acid, and cytokinin in walnut. The qRT-PCR validated the results of differential expression analysis and further provided three JrASMT genes with different expression profiles in walnut flower bud development. Our study explored the evolutionary relationships of the plant ASMT gene family and the functional characteristics of walnut JrASMT. It provides a valuable perspective for further understanding the complex melatonin mechanisms in plant developmental regulation.

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Genome‑wide identification and expression analysis of the ASMT gene family reveals their role in abiotic stress tolerance in apple

Cited by 19 publications

References 43 publications

Genetic and evolutionary dissection of melatonin response signaling facilitates the regulation of plant growth and stress responses

Genetic and evolutionary dissection of melatonin response signaling facilitates the regulation of plant growth and stress responses

Melatonin Induced Cold Tolerance in Plants: Physiological and Molecular Responses

Walnut N-Acetylserotonin Methyltransferase Gene Family Genome-Wide Identification and Diverse Functions Characterization During Flower Bud Development

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