Genome-Wide Identification and Expression Analysis of Respiratory Burst Oxidase Homolog (RBOH) Gene Family in Eggplant (Solanum melongena L.) under Abiotic and Biotic Stress

Abstract

Respiratory burst oxidase homologs (RBOHs) are important proteins that catalyze the production of reactive oxygen species (ROS), which play important roles in growth and stress response. For a comprehensive analysis of SmRBOH genes, we conducted genome-wide identification of the SmRBOH gene family in eggplant (Solanum melongena L.) and analyzed the expression of SmRBOHs under abiotic (salt, high-temperature, and low-temperature) and biotic stress (Verticillium dahliae inoculation) by quantitative real-time PCR (qRT-PCR). The result showed that a total of eight SmRBOH members were identified from the genome database of eggplant, and they were relatively evenly distributed across seven chromosomes. The analysis of Motif and the conserved domain showed that SmRBOHs have high similarity in protein sequences and functions. Based on phylogenetics, SmRBOHs were classified into three distinct clades. Furthermore, the promoter regions of SmRBOHs were found to contain different cis-elements. Additionally, the results of the qRT-PCR demonstrated differential expression patterns of SmRBOHs in different tissues (the roots, stems, and leaves) and stress conditions. SmRBOHB, SmRBOHD, SmRBOHH1, and SmRBOHH2 showed significant upregulation (>20-fold) under at least one stress condition. Subcellular localization analysis of the above four members further confirmed that they localized on the plasma membrane. This study provides a theoretical foundation for understanding the functions of SmRBOHs in eggplant.

Genome wide comprehensive characterization and expression analysis of RBOH genes revealed drought stress response in mung bean (Vigna radiate L.)

“…Similarly, the N-terminal regions of the VraRBOH proteins contain NADPH oxidase domain and two putative calcium binding regions (EF-hands) for playing a key role in the regulation of RBOHs. The VraRBOH proteins also contain 9 transmembrane domains that resembles to those identi ed in various crops (Gui et al 2022;Du et al 2023). Besides, VraRBOH proteins also possessed ferric reductase, FAD-binding domain 8, and NADbinding 6 domains.…”

“…Even, in case of other plants, few plant species possessed the intronless gene structure in RBOH gene family (Hu et al 2018;Cheng et al 2020). In contrast, the divergence in RBOH gene structure was observed in many plants (Du et al 2023). Similarly, the VraRBOH4 gene possessed 23 introns and longest intron too in the present investigation.…”

“…Similarly, the RBOH gene promoters of other plants reported to possess drought responsive cis-regulatory elements along with many biotic and abiotic stress responsive cis-regulatory elements. For example, the up-stream region of RBOHE1, RBOHH1 and RBOHH2 genes of brinjal contained the drought-responsive MBS elements (Du et al 2023). Similarly, the CsRBOHC gene promoter of citrus also showed the presence of drought responsive cis-regulatory element (Zhang et al 2022).…”

“…them(Wang et al 2016;Shi et al 2020;Du et al 2023;Sharma et al 2024). The activity of RBOH genes was increased by drought and high-temperature stress suggesting its important role in response to drought stress in plants(Duan et al 2009;Zhang et al 2023).…”

Genome-wide analysis of respiratory burst oxidase homolog gene family in pea (Pisum sativum L.)

“…With the recent increase in whole-genome sequencing of more plant species, RBOH genes have been functionally characterized in various plant species. These plants include Arabidopsis thaliana ( Torres et al., 1998 ; Torres et al., 2002 ), Lycopersicon esculentum ( Sagi et al., 2004 ), Olea europaea ( Jimenez-Quesada et al., 2019 ), Glycine max ( Liu et al., 2019 ), Brassica rapa ( Li et al., 2019 ), Capsicum annuum ( Zhang et al., 2021 ), Pyropia yezoensis ( Gui et al., 2022 ), and Solanum melongena ( Du et al., 2023 ).…”