Genome-wide identification and expression analysis of the EXO70 gene family in grape (Vitis vinifera L)

Abstract

EXO70 is the pivotal protein subunit of exocyst, which has a very crucial role in enhancing the shielding effect of the cell wall, resisting abiotic and hormonal stresses. This experiment aims to identify family members of the EXO70 gene family in grape and predict the characteristics of this gene family, so as to lay the foundation of further exploring the mechanism of resisting abiotic and hormone stresses of VvEXO70s. Therefore, the Vitis vinifera ‘Red Globe’ tube plantlet were used as materials. Bioinformatics was used to inquire VvEXO70 genes family members, gene structure, system evolution, cis-acting elements, subcellular and chromosomal localization, collinearity, selective pressure, codon bias and tissue expression. All of VvEXO70s had the conserved pfam03081 domain which maybe necessary for interacting with other proteins. Microarray analysis suggested that most genes expressed to varying degrees in tendrils, leaves, seeds, buds, roots and stems. Quantitative Real-Time PCR (qRT-PCR) showed that the expression levels of all genes with 5 mM salicylic acid (SA), 0.1 mM methy jasmonate (MeJA), 20% PEG6000 and 4 °C for 24 h were higher than for 12 h. With 20% PEG6000 treatment about 24 h, the relative expression of VvEXO70-02 was significantly up-regulated and 361 times higher than CK. All genes’ relative expression was higher at 12 h than that at 24 h after treatment with 7 mM hydrogen peroxide (H2O2) and 0.1 mM ethylene (ETH). In conclusion, the expression levels of 14 VvEXO70 genes are distinguishing under these treatments, which play an important role in the regulation of anti-stress signals in grape. All of these test results provide a reference for the future research on the potential function analysis and plant breeding of VvEXO70 genes.

Association Study of Agronomic and Oil Traits in Rapeseed (Brassica napus L.) Mutant Lines Using Genotyping-by-Sequencing

“…A total of six genes (EXO70, Prm, MED34, CCNC, RNASET2 and EDR2) were annotated from erucic acid. The EXO70 encodes for proteins that are involved in the formation of the exocyst complex, which is responsible for the targeted delivery of membrane vesicles to specific locations on the plasma membrane [58]. The exocyst complex is essential for a variety of cellular processes, including cell polarity, cell division, and secretion [59].…”

“…The exocyst complex is essential for a variety of cellular processes, including cell polarity, cell division, and secretion [59]. The EXO70 encodes for a group of proteins that are involved in the formation of the exocyst complex, which is essential for the transport of secretory vesicles to the plasma membrane in eukaryotic cells [58,59]. While the role of the EXO70 family in exocytosis is well established, there is also evidence suggesting that some members of the family may be involved in lipid transport and secretion metabolism, the expression of EXO70 family (EXO70H4) was upregulated in response to glucose and that plants with mutations in the gene had reduced levels of fatty acids in Arabidopsis thaliana [58,60].…”

“…The EXO70 encodes for a group of proteins that are involved in the formation of the exocyst complex, which is essential for the transport of secretory vesicles to the plasma membrane in eukaryotic cells [58,59]. While the role of the EXO70 family in exocytosis is well established, there is also evidence suggesting that some members of the family may be involved in lipid transport and secretion metabolism, the expression of EXO70 family (EXO70H4) was upregulated in response to glucose and that plants with mutations in the gene had reduced levels of fatty acids in Arabidopsis thaliana [58,60]. Another study found that the EXO70A1 protein in the filamentous fungus Aspergillus nidulans is involved in the regulation of lipid metabolism that mutations in the gene resulted in altered lipid composition, including a decrease in fatty acid content [58][59][60].…”

“…While the role of the EXO70 family in exocytosis is well established, there is also evidence suggesting that some members of the family may be involved in lipid transport and secretion metabolism, the expression of EXO70 family (EXO70H4) was upregulated in response to glucose and that plants with mutations in the gene had reduced levels of fatty acids in Arabidopsis thaliana [58,60]. Another study found that the EXO70A1 protein in the filamentous fungus Aspergillus nidulans is involved in the regulation of lipid metabolism that mutations in the gene resulted in altered lipid composition, including a decrease in fatty acid content [58][59][60]. The specific mechanisms by which the EXO70 family members regulate rapeseed fatty acid biosynthesis are not yet fully understood, these studies suggest that they may play a role in this important metabolic process.…”

Genome-Wide Identification and Expression Analysis of Brassica EXO70 Gene Family

“…Fourteen EXO70 genes were identified in Vitis vinifera, and most of these were determined to be expressed in the pollen, tendrils, leaves, seeds, buds, roots, and stems of the plants [35].…”