Genome-wide Identification and Expression Analysis of Amino Acid Transporters in the Whitefly, <i>Bemisia tabaci </i>(Gennadius)

Xia, Jixing; Yang, Zezhong; Chen, Gong; Xie, Wen; Pan, Huipeng; Guo, Zhaojiang; Zheng, Huixin; Yang, Xin; Sun, Xiaodong; Kang, Shi; Fang, Yang; Wu, Qingjun; Wang, Shaoli; Cong, Bin; Teng, Xianfeng; Zhang, Youjun

doi:10.7150/ijbs.18153

Cited by 12 publications

(20 citation statements)

References 66 publications

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“…Knockdown of BTbe5, BTbe3, BTjhe1, BTjhe2, BTae2, and BTun2 genes was performed by orally feeding dsRNA to B. tabaci MED adults in feeding chambers. The feeding chambers contained 0.2 mL of diet solution, which contained 30% sucrose, 5% yeast extract (weight/volume), with 0.5 μg/μL dsBTbe5, dsBTbe3, dsBTjhe1, dsBTjhe2, dsBTae2, and dsBTun2 [69]. Approximately 60 newly emerged (<2 days old) B. tabaci MED adults (mixed sexes) were released into the feeding chambers, and were kept at 25 °C, 80% RH, and an L14:D10 photoperiod.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius)

Xia

Yang

et al. 2019

IJMS

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The whitefly (Bemisia tabaci), an important invasive pest that causes severe damage to crops worldwide, has developed resistance to a variety of insecticides. Carboxylesterases (COEs) are important multifunctional enzymes involved in the growth, development, and xenobiotic metabolism of insects. However, systematic studies on the COEs of B. tabaci are scarce. Here, 42 putative COEs in different functional categories were identified in the Mediterranean species of B. tabaci (B. tabaci MED) based on a genome database and neighbor-joining phylogeny. The expression patterns of the COEs were affected by the development of B. tabaci. The expression levels of six COEs were positively correlated with the concentration of imidacloprid to which B. tabaci adults were exposed. The mortality of B. tabaci MED adults fed dsBTbe5 (67.5%) and dsBTjhe2 (58.4%) was significantly higher than the adults fed dsEGFP (41.1%) when treated with imidacloprid. Our results provide a basis for functional research on COEs in B. tabaci and provide new insight into the imidacloprid resistance of B. tabaci.

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius)

Xia

Yang

et al. 2019

IJMS

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“…Other groups, such as the Sugar Porter (SP), the Solute:Sodium Symporter (SSS), and the SWEET families have been implicated in sugar transport [13]. Different labs have adopted different nomenclatures for these transporters (see Additional file 5: Table S1), but this has not prevented several groups from identifying members of these transporter families in insects [14][15][16], although this has not been performed in any stink bug species.…”

Section: Introductionmentioning

confidence: 99%

A transcriptomic and proteomic atlas of expression in the Nezara viridula (Heteroptera: Pentatomidae) midgut suggests the compartmentalization of xenobiotic metabolism and nutrient digestion

et al. 2020

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Background: Stink bugs are an emerging threat to crop security in many parts of the globe, but there are few genetic resources available to study their physiology at a molecular level. This is especially true for tissues such as the midgut, which forms the barrier between ingested material and the inside of the body. Results: Here, we focus on the midgut of the southern green stink bug Nezara viridula and use both transcriptomic and proteomic approaches to create an atlas of expression along the four compartments of the anterior-posterior axis. Estimates of the transcriptome completeness were high, which led us to compare our predicted gene set to other related stink bugs and Hemiptera, finding a high number of species-specific genes in N. viridula. To understand midgut function, gene ontology and gene family enrichment analyses were performed for the most highly expressed and specific genes in each midgut compartment. These data suggested a role for the anterior midgut (regions M1-M3) in digestion and xenobiotic metabolism, while the most posterior compartment (M4) was enriched in transmembrane proteins. A more detailed characterization of these findings was undertaken by identifying individual members of the cytochrome P450 superfamily and nutrient transporters thought to absorb amino acids or sugars. Conclusions: These findings represent an initial step to understand the compartmentalization and physiology of the N. viridula midgut at a genetic level. Future studies will be able to build on this work and explore the molecular physiology of the stink bug midgut.

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“…Other studies have annotated individual SLC families, but on an ad hoc basis and often using different nomenclature ( Price et al. 2010 ; Xia et al. 2017 ; Yang et al.…”

Section: Introductionmentioning

confidence: 99%

The Identification and Evolutionary Trends of the Solute Carrier Superfamily in Arthropods

Denecke

Driva

Lương

et al. 2020

Genome Biology and Evolution

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The Solute Carrier (SLC) transporter superfamily comprises an ancient and ubiquitous group of proteins capable of translocating a range of nutrients, endogenous molecules, and xenobiotics. Although the group has been the subject of intense investigation and identification in both bacteria and mammals, its systematic investigation in arthropods has not yet been undertaken. Here, we present a genome-wide identification of all 66 SLC families in 174 arthropod species. A pipeline (SLC_id) was constructed to identify and group SLCs using a combination of hidden Markov model and BLAST searches followed by filtering based on polypeptide length and the number of transmembrane domains. Comparative analysis of the number of transporters in each family across diverse arthropod lineages was accomplished using one-way analysis of variance (ANOVA) and the Computational Analysis of Family Expansion (CAFE). These results suggested that many SLC families have undergone expansions or contractions in particular evolutionary lineages. Notably, the sugar transporting SLC2 family was significantly larger in insects compared to arachnids. This difference may have been complemented by a rapid expansion of the SLC60 family in arachnids which also acts on dietary sugars. Furthermore, the SLC33 family underwent a recent and drastic expansion in aphids, although the biological relevance of this expansion was not possible to infer. Information on specific SLC transporter families across arthropod species can be accessed through an R shiny web application at http://chrysalida.imbb.forth.gr:3838/Arthropod_SLC_Database/. The present study greatly facilitates further study of the understudied and diverse group of SLC transporters in arthropods.

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Genome-wide Identification and Expression Analysis of Amino Acid Transporters in the Whitefly, Bemisia tabaci (Gennadius)

Cited by 12 publications

References 66 publications

Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius)

Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius)

A transcriptomic and proteomic atlas of expression in the Nezara viridula (Heteroptera: Pentatomidae) midgut suggests the compartmentalization of xenobiotic metabolism and nutrient digestion

The Identification and Evolutionary Trends of the Solute Carrier Superfamily in Arthropods

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