Genome-Wide Identification and Expression Analysis of the 14-3-3 Gene Family in Mango (Mangifera indica L.)

Abstract

Members of the Mi14-3-3 gene family interact with target proteins that are widely involved in plant hormone signal transduction and physiology-related metabolism and play important roles in plant growth, development and stress responses. In this study, 14-3-3s family members are identified by the bioinformatic analysis of the mango (Mangifera indica L.) genome. The gene structures, chromosomal distributions, genetic evolution, and expression patterns of these genes and the physical and chemical properties and conserved motifs of their proteins are analysed systematically. The results identified 16 members of the 14-3-3 genes family in the mango genome. The members were not evenly distributed across the chromosomes, and the gene structure analysis showed that the gene sequence length and intron number varied greatly among the different members. Protein sequence analysis showed that the Mi14-3-3 proteins had similar physical and chemical properties and secondary and tertiary structures, and protein subcellular localization showed that the Mi14-3-3 family proteins were localized to the nucleus. The sequence analysis of the Mi14-3-3s showed that all Mi14-3-3 proteins contain a typical conserved PFAM00244 domain, and promoter sequence analysis showed that the Mi14-3-3 promoters contain multiple hormone-, stress-, and light-responsive cis-regulatory elements. Expression analysis showed that the 14-3-3 genes were expressed in all tissues of mango, but that their expression patterns were different. Drought, salt and low temperature stresses affected the expression levels of 14-3-3 genes, and different 14-3-3 genes had different responses to these stresses. This study provides a reference for further studies on the function and regulation of Mi14-3-3 family members.

Genome-wide identification of the mango CONSTANS (CO) family and functional analysis of two MiCOL9 genes in transgenic Arabidopsis

“…In total, 31 putative CO genes were identified from the mango genome (unpublished) ( Xia et al., 2022 ; Zhu et al., 2022 ) using sequences of Arabidopsis CO genes as a reference ( Table 1 ). The conserved domains of the target sequences were evaluated by bioinformatic analysis, and all putative genes were subsequently verified with HMMER and SMART (Simple Modular Architecture Research Tool) online software.…”

Comprehensive Identification and Analyses of the GRF Gene Family in the Whole-Genome of Four Juglandaceae Species

“…In addition, the Juglandaceae GRF protein family triple structure consists mainly of α-helices, and the phylogenetic relationships of Juglandaceae GRF gene family members can be classified into ε-like and non-ε-like classes, with no amino acid loci detected that are subject to positive selection pressure. Gene structure analysis showed that ε-like GRF genes contain more exons and introns than non-ε-like GRF genes, suggesting that this evolution has driven this diversity, and the above results are similar to those of the mango 14-3-3 family of proteins [ 35 ]. The vast majority of the Juglandaceae GRF family have similar and consistent motif patterns between the ε and non-ε groups, but some members of the non-ε group show considerable variation, containing only Motif1 and Motif2, which may be related to the evolution of individual Juglandaceae species.…”

Genome-wide analysis of the G-box regulating factors protein family reveals its roles in response to Sclerotinia sclerotiorum infection in rapeseed (Brassica napus L.)

“…Cis-regulatory elements play an important role in the regulation of plant gene expression. The cis-acting element and expression pattern analysis showed that 14-3-3s were involved in hormone and abiotic stress responses in plants (Lyu et al, 2021;Xia et al, 2022;Xie et al, 2022). In this study, we analyzed the stress response-related cis-acting elements in the promoters of BnaGRFs.…”

“…A number of 14-3-3 family members have been reported in different plant species; for example, 13 in Arabidopsis (Rosenquist et al, 2001), 8 in rice (Chen et al, 2006), 16 in soybean (Wang et al, 2019), 17 in tobacco (Konagaya et al, 2004), 12 in Populus (Tian et al, 2015), 8 in sweet orange, 15 in cassava (Chang et al, 2020), 11 in grape (Cheng et al, 2018), 17 in wheat (Shao et al, 2021), 10 in Medicago truncatula (Qin et al, 2016), 16 in mango (Xia et al, 2022), 18 in apple (Zuo et al, 2021), 25 in banana (Li et al, 2016), 9 in Citrus sinensis (Lyu et al, 2021), and 21 in Brassica rapa (Chandna et al, 2016). Despite the documentation of 14-3-3 genes in many plant species and their investigation under different stress conditions, little is known about this gene family in rapeseed (Brassica napus) and its diploid progenitor Brassica oleracea.…”