Genome-wide identification and expression analysis of the CLC superfamily genes in tea plants (Camellia sinensis)

Xing, Anqi; Ma, Yuanchun; Wu, Zichen; Nong, Shouhua; Zhu, Jiaojiao; Sun, Hong; Tao, Jing; Wen, Bo; Zhu, Xujun; Fang, Wanping; Li, Xiaocheng; Wang, Yuhua

doi:10.1007/s10142-019-00725-9

Cited by 16 publications

(15 citation statements)

References 48 publications

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“…1). The CLCs topology was consistent with that of Arabidopsis [28,29], tobacco [40], tea (Camellia sinensis) [19] and trifoliate orange [18]. Phylogenetic analysis also indicated multiple rounds of ancient gene expansion (Fig.…”

Section: Phylogenic Relationships Of the Clc Gene Familysupporting

confidence: 72%

“…CLC proteins may act as Cl − channels or as Cl − /H + -exchangers (antiporters) [14]. The CLC gene family contains three highly conserved regions related to anion selectivity: GxGIPE (I), GKxGPxxH (II) and PxxGxLF (III) [19]. If the x residue in the conserved region (I) is P [proline, Pro], NO 3 − is preferentially transported, whereas if it is substituted by S [serine, Ser], Cl − is preferentially transported [20].…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification and expression analysis of the CLC gene family in pomegranate (Punica granatum) reveals its role in salt resistance

Liu

Zhao

et al. 2020

Preprint

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BackgroundsPomegranate (Punica granatum L.) is an important commercial fruit tree, with moderate tolerance to salinity. The balance of Cl− and other anions in pomegranate tissues are affected by salinity, however, the accumulation patterns of anions are poorly understood. The chloride channel (CLC) gene family is involved in conducting Cl−, NO3−, HCO3− and I−, but its characteristics have not been reported on pomegranate.ResultsIn this study, we identified seven PgCLC genes, consisting of four antiporters and three channels, based on the presence of the gating glutamate (E) and the proton glutamate (E). Phylogenetic analysis revealed that seven PgCLCs were divided into two clades, with clade I containing the typical conserved regions GxGIPE (I), GKxGPxxH (II) and PxxGxLF (III), whereas clade II not. Multiple sequence alignment revealed that PgCLC-B had a P [proline, Pro] residue in region I, which is suspected to be a NO3–/H+ exchanger, while PgCLC-C1, PgCLC-C2, PgCLC-D and PgCLC-G contained a S [serine, Ser] residue, with a high affinity to Cl−. We were determined the content of Cl−, NO3−, H2PO4−, and SO42− in pomegranate tissues after 18 days of salt treatments (0, 100, 200 and 300 mM NaCl). Compared with control, the Cl− content increased sharply in tissues and was ranked as leaf > stem > root. The uptake of NO3− and SO42− was inhibited by high salinity, while that of H2PO4− increased. The results of real-time reverse transcription PCR (qRT-PCR) revealed that PgCLC genes had tissue-specific expression patterns. The high expression levels of PgCLC-C1, PgCLC-C2 and PgCLC-D in leaves suggested they played roles in sequestrating Cl− into the vacuoles. However, the low expression levels of PgCLCs in roots might be contributed to the exclusion of Cl− from root cells. Also, the non-significantly changed concentration of NO3− in leaves and the up-regulated PgCLC-B indicated an acceleration of transporting NO3− into leaves to mitigate the nitrogen deficiency.ConclusionsOur findings suggested that PgCLC genes played important roles in balance of Cl− and NO3− in pomegranate tissues under salt stress. This study establishes a theoretical foundation for the further functional characterization of CLC genes in pomegranate.

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Section: Phylogenic Relationships Of the Clc Gene Familysupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of the CLC gene family in pomegranate (Punica granatum) reveals its role in salt resistance

Liu

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…1). The CLCs topology was consistent with that of Arabidopsis [27,28], tobacco [38], tea (Camellia sinensis) [19] and trifoliate orange [18]. Phylogenetic analysis also indicated multiple rounds of ancient gene expansion (Fig.…”

Section: Phylogenic Relationships Of the Clc Gene Familysupporting

confidence: 72%

“…All of the CLC proteins have a highly conserved voltage-gated chloride channel (Voltage-gate CLC) domain and two CBS (cystathionine beta synthase) domains of putative regulatory function [9]. Also, the CLC gene family members contain three highly conserved regions related to anion selectivity: GxGIPE (I), GKxGPxxH (II) and PxxGxLF (III) [19]. If the x residue in the conserved region (I) is P [proline, Pro], NO 3 is preferentially transported, whereas if it is substituted by S [serine, Ser], Clis preferentially transported [20].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of the CLC gene family in pomegranate (Punica granatum) reveals its roles in salt resistance

Liu

Zhao

et al. 2020

Preprint

View full text Add to dashboard Cite

Backgrounds: Pomegranate (Punica granatum L.) is an important commercial fruit tree, with moderate tolerance to salinity. The balance of Cl− and other anions in pomegranate tissues are affected by salinity, however, the accumulation patterns of anions are poorly understood. The chloride channel (CLC) gene family is involved in conducting Cl−, NO3−, HCO3− and I−, but its characteristics have not been reported on pomegranate.Results: In this study, we identified seven PgCLC genes, consisting of four antiporters and three channels, based on the presence of the gating glutamate (E) and the proton glutamate (E). Phylogenetic analysis revealed that seven PgCLCs were divided into two clades, with clade I containing the typical conserved regions GxGIPE (I), GKxGPxxH (II) and PxxGxLF (III), whereas clade II not. Multiple sequence alignment revealed that PgCLC-B had a P [proline, Pro] residue in region I, which was suspected to be a NO3–/H+ exchanger, while PgCLC-C1, PgCLC-C2, PgCLC-D and PgCLC-G contained a S [serine, Ser] residue, with a high affinity to Cl−. We determined the content of Cl−, NO3−, H2PO4−, and SO42− in pomegranate tissues after 18 days of salt treatments (0, 100, 200 and 300 mM NaCl). Compared with control, the Cl− content increased sharply in pomegranate tissues. Salinity inhibited the uptake of NO3− and SO42− , but accelerated H2PO4− uptake. The results of real-time reverse transcription PCR (qRT-PCR) revealed that PgCLC genes had tissue-specific expression patterns. The high expression levels of three antiporters PgCLC-C1, PgCLC-C2 and PgCLC-D in leaves might be contributed to sequestrating Cl− into the vacuoles. However, the low expression levels of PgCLCs in roots might be associated with the exclusion of Cl− from root cells. Also, the up-regulated PgCLC-B in leaves indicated that more NO3− was transported into leaves to mitigate the nitrogen deficiency.Conclusions: Our findings suggested that the PgCLC genes played important roles in balancing of Cl− and NO3− in pomegranate tissues under salt stress. This study establishes a theoretical foundation for the further functional characterization of CLC genes in pomegranate.

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“…Different CLC proteins function as anion/H + exchangers or anion-selective channels. In reports, AtCLCa has been characterized as a twoanion/H + exchanger which drives the active uptake of anions inside the vacuoles of Arabidopsis guard cells and mesophyll with higher selectivity for NO 3 − ions over Cl − ions [54]. Besides, CLCs play a vital role in loading anions in the vacuole of guard cells for stomatal opening in response to light and later releasing them during ABA-induced stomatal closure [55].…”

Section: Ion Stress Signaling and Homeostasismentioning

confidence: 99%

Abiotic Stress Responses in Plants: Current Knowledge and Future Prospects

Marothia¹,

Kaur²,

Pati³

2021

Abiotic Stress in Plants

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Exposure to abiotic stresses has become a major threatening factor that hurdles the sustainable growth in agriculture for fulfilling the growing food demand worldwide. A significant decrease in the production of major food crops including wheat, rice, and maize is predicted in the near future due to the combined effect of abiotic stresses and climate change that will hamper global food security. Thus, desperate efforts are necessary to develop abiotic stress-resilient crops with improved agronomic traits. For this, detailed knowledge of the underlying mechanisms responsible for abiotic stress adaptation in plants is must required. Plants being sessile organisms respond to different stresses through complex and diverse responses that are integrated on various whole plants, cellular, and molecular levels. The advanced genetic and molecular tools have uncovered these complex stress adaptive processes and have provided critical inputs on their regulation. The present chapter focuses on understanding the different responses of the plants involved in abiotic stress adaptation and strategies employed to date for achieving stress resistance in plants.

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Genome-wide identification and expression analysis of the CLC superfamily genes in tea plants (Camellia sinensis)

Cited by 16 publications

References 48 publications

Genome-wide identification and expression analysis of the CLC gene family in pomegranate (Punica granatum) reveals its role in salt resistance

Genome-wide identification and expression analysis of the CLC gene family in pomegranate (Punica granatum) reveals its role in salt resistance

Genome-wide identification and expression analysis of the CLC gene family in pomegranate (Punica granatum) reveals its roles in salt resistance

Abiotic Stress Responses in Plants: Current Knowledge and Future Prospects

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