Genome-wide identification and comparison of legume MLO gene family

Rispail, Nicolás; Rubiales, Diego

doi:10.1038/srep32673

Cited by 45 publications

(83 citation statements)

References 59 publications

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“…From a breeding point of view, the first step is to identify and characterize the MLO genes present in a particular crop species followed by the development of breeding tools, such as genetic markers, for their use in crop improvement. This interest is highlighted by the set of recently-published papers devoted to the identification and characterization of these genes and/or validation of their associated markers: Solanum species [ 17 ], pigeon pea and common bean [ 18 ], Vitis flexuosa [ 19 ], legume species [ 20 ], pea [ 21 – 22 ], cotton [ 23 ], tomato [ 24 ], cucumber [ 25 ], and chick pea [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

A genome-wide identification and comparative analysis of the lentil MLO genes

et al. 2018

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Powdery mildew is a widespread fungal plant disease that can cause significant losses in many crops. Some MLO genes (Mildew resistance locus O) have proved to confer a durable resistance to powdery mildew in several species. Resistance granted by the MLO gene family members has prompted an increasing interest in characterizing these genes and implementing their use in plant breeding. Lentil (Lens culinaris Medik.) is a widely grown food legume almost exclusively consumed as dry seed with an average world production of 4.5 million tons. Powdery mildew causes severe losses on certain lentil cultivars under particular environmental conditions. Data mining of the lentil CDC Redberry draft genome allowed to identify up to 15 gene sequences with homology to known MLO genes, designated as LcMLOs. Further characterization of these gene sequences and their deduced protein sequences demonstrated conformity with key MLO protein characteristics such as the presence of transmembrane and calmodulin binding domains, as well as that of other conserved motifs. Phylogenetic and other comparative analyses revealed that LcMLO1 and LcMLO3 are the most likely gene orthologs related to powdery mildew response in other species, sharing a high similarity with other known resistance genes of dicot species, such as pea PsMLO1 and Medicago truncatula MtMLO1 and MtMLO3. Sets of primers were designed as tools to PCR amplify the genomic sequences of LcMLO1 and LcMLO3, also to screen lentil germplasm in search of resistance mutants. Primers were used to obtain the complete sequences of these two genes in all of the six wild lentil relatives. Respective to each gene, all Lens sequences shared a high similarity. Likewise, we used these primers to screen a working collection of 58 cultivated and 23 wild lentil accessions in search of length polymorphisms present in these two genes. All these data widen the insights on this gene family and can be useful for breeding programs in lentil and close related species.

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Section: Introductionmentioning

confidence: 99%

A genome-wide identification and comparative analysis of the lentil MLO genes

et al. 2018

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“…The in silico analysis allowed the identification of 16 FvMLO genes and the phylogenetic analysis of FvMLO proteins performed with MLO family members of grapevine, Arabidopsis, and selected monocot and dicot MLO proteins revealed that the proteins were grouped into six distinct clades, two of which (III and V) comprise members that are associated with powdery mildew susceptibility in dicots and monocots, respectively. In this study, it was observed that the number of FvMLO genes and clade numbering of the FvMLO gene family were in contrast to the previously categorized FvMLO genes.…”

Section: Discussionmentioning

confidence: 99%

“…An MLO gene was first identified in barley [3] and currently, a varying number of MLO genes have been identified in Arabidopsis, Medicago truncatula, chickpea, rice, maize, wheat, soybean, cucumber, tomato, rose, grapevine, peach, apple, sweet orange, cultivated Solanaceae, Brachypodium, and Cucurbitaceae species respectively [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Although the biological functions of MLO genes are not yet completely known, they act as suppressors of defense responses.…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression Analysis of Fragaria Vesca MLO Genes Involved in Interaction with Powdery Mildew (Podosphaera Aphanis)

Jambagi

Dunwell

2017

JAPB

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Strawberry powdery mildew, caused by Podosphaera aphanis is a major fungal disease that affects strawberry yield and quality. In the model plant species Arabidopsis and the crop plants barley, tomato and pea, the Mildew resistance locus O (MLO) proteins have been found to be required for powdery mildew susceptibility.The present study, based on the sequence of a wild plum (Prunus americana) MLO protein, identified 16 MLO genes within the genome of woodland strawberry, Fragaria vesca and examined their expression pattern in response to powdery mildew infection in three diploid strawberry cultivars. Phylogenetic analysis showed that the FvMLO genes can be classified into six clades. Four FvMLO genes were grouped into clade III, which comprises MLO genes from Arabidopsis, tomato and grapevine that mediate powdery mildew susceptibility. A RNA-seq analysis of two diploid strawberry cultivars, F. vesca ssp. vesca accession Hawaii 4 (HW) and F. vesca f. semperflorens line "Yellow Wonder 5AF7" (YW) at 1 d (1 DAI) and 8 d (8 DAI) after infection showed the expression of 12 out of the 16 FvMLO genes. The comparison of Fragments Per Kilobase of transcript per Million mapped reads (FPKM values) detected by RNA-seq and expression values of qRT-PCR for FvMLO genes showed substantial agreement. The FvMLO3 gene, which was grouped in clade III and orthologous to the Arabidopsis, tomato and grapevine genes, was highly expressed in YW compared to other FvMLO genes across varieties. The results showed that FvMLO genes can be used as potential candidates to engineer powdery mildew resistance in strawberry based on MLO suppression or genome editing.

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“…Loss of function studies in some mlo genes have conferred a durable and broad-spectrum, recessively inherited resistance in Arabidopsis [13], cucumber [14], tomato [15,16] and grapevines [17]. Comparative genomics approach has revealed the presence of ve Mlo loci in the Common bean genome that could be associated with PM resistance as clustered on Clade V along with their orthologs [18]. However, their functionality has not been validated yet.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Identification of Powdery Mildew Resistance in Common Bean

Binagwa

Traore

Egnin

et al. 2020

Preprint

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Background: Genome-wide association studies (GWAS) was utilized to detect genetic variations related to the powdery mildew (PM) resistance and several agronomic traits in common bean. However, its application in common bean and the PM interactions to identify genes and their location in the common bean genome has not been fully addressed. Results: Genome-wide association studies (GWAS) through marker-trait association are useful molecular tools for the identification of disease resistance and other agronomic traits. SNP genotyping with a BeadChip containing 5398 SNPs was used to detect genetic variations related to resistance to PM disease in a panel of 206 genotypes grown under field conditions for two consecutive years. Significant SNPs identified on chromosomes 4 and 10 (Pv04 and Pv10) were repeatable, confirming the reliability of the phenotypic data scored from the genotypes grown in two locations within two years. A cluster of resistance genes was revealed on the chromosome 4 of common bean genome among which CNL and TNL like resistance genes were identified. Furthermore, two resistance genes Phavu_010G1320001g and Phavu_010G136800g were also identified on Pv10; further sequence analysis showed that these genes were homologs to the Arabidopsis disease resistance protein (RLM1A-like) and the putative disease resistance protein (At4g11170.1), respectively. Two LRR receptor-like kinases (RLK) were also identified on Pv11 in samples collected in 2018 only. Many genes encoding auxin-responsive protein, TIFY10A protein, growth-regulating factor 5-like, ubiquitin-like protein, cell wall protein RBR3-like protein related to PM resistance were identified nearby significant SNPs. These results suggested that the resistance to PM pathogen involves a network of many genes constitutively co-expressed and may generate several layers of defense barriers or inducible reactions.Conclusion: Our results provide new insights into common bean and PM interactions, and revealed putative resistance genes as well as their location on common bean genome that could be used for marker-assisted selection, functional genomic study approaches to confirm the role of these putative genes; hence, developing common bean resistance lines to the PM disease.

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Genome-wide identification and comparison of legume MLO gene family

Cited by 45 publications

References 59 publications

A genome-wide identification and comparative analysis of the lentil MLO genes

A genome-wide identification and comparative analysis of the lentil MLO genes

Identification and Expression Analysis of Fragaria Vesca MLO Genes Involved in Interaction with Powdery Mildew (Podosphaera Aphanis)

Genome-wide Identification of Powdery Mildew Resistance in Common Bean

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