Genome-wide identification and characterization of AINTEGUMENTA-LIKE (AIL) family genes in apple (Malus domestica Borkh.)

Han, Xiaolei; Yuan, Gaopeng; He, Shikun; Peng, Cong; Zhang, Caixia

doi:10.1016/j.ygeno.2022.110313

Cited by 10 publications

(20 citation statements)

References 59 publications

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“…Their high expression levels were maintained to the end of the cell division stage to propel the division of competent undifferentiated cells. However, meristematic cells eventually lose their competence with both ANT variants become down-regulated as the fruit enters the ripening stage [1,2,21]. These observations were nonetheless critical in demonstrating that the cyclical expression of MdANT1 and MdANT2 coincided with the developmental stage of cell division during fruit growth.…”

Section: Introductionmentioning

confidence: 99%

“…Apple fruit size is dependent on its cell number and size, characteristics regulated by cell division and expansion rates [1,2]. Although the average-sized fruit has approximately 50 million of cells, some grow larger by either increasing their cell numbers or enlarging their cells [3].…”

Section: Introductionmentioning

confidence: 99%

“…Our interest in utilising CDKB2;2 as a marker of cell division therein lies in its distinct down-regulation towards the end of the early developmental stages, an observation which may have alluded to the participation of CDKB2;2 in the cell cycle regulation of the apple fruit. In Arabidopsis, ANT was demonstrated to control cell proliferation in integuments during ovule growth [2,21]. The loss of ANT function decreased cell division activity, resulting in reduced floral organ size.…”

Section: Introductionmentioning

confidence: 99%

“…These observations were nonetheless critical in demonstrating that the cyclical expression of MdANT1 and MdANT2 coincided with the developmental stage of cell division during fruit growth. Both genes are known to be more lengthily and highly expressed in larger fruits within the cultivar Golden Delicious Smoothee (GS) [1,2]. Moreover, MdANT1 and MdANT2 have exhibited a positive correlation to A and B-type CDKs during the regulation of cell production and cell cycle in apples [1,2].…”

Section: Introductionmentioning

confidence: 99%

“…Both genes are known to be more lengthily and highly expressed in larger fruits within the cultivar Golden Delicious Smoothee (GS) [1,2]. Moreover, MdANT1 and MdANT2 have exhibited a positive correlation to A and B-type CDKs during the regulation of cell production and cell cycle in apples [1,2].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Cell division controls final fruit size in three apple (Malus x domestica) cultivars

Karim¹,

Allan²,

Schaffer³

et al. 2022

Preprint

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Apple (Malus x domestica) fruit size is dependent on cell division and cell expansion, processes which are subsequently regulated by plant hormones such as auxins, gibberellins, and cytokinins. In this study, we investigated the role of cell division and cell expansion in apple growth and identified which of the two was more deterministic to final fruit size. Three cultivars of different sizes were selected, namely, ‘Twenty Ounce’ (large-sized), ‘Royal Gala’ (medium-sized), and ‘Crabapple’ (small-sized). Gene expression and cell size analyses were conducted over the course of two consecutive seasons. The expression patterns of three classes of genes were markedly similar across all cultivars. Two cell division markers, namely, MdCDKB2;2 and MdANT2, were discovered to be correlatively expressed as both displayed initially high expression levels, which gradually declined from the early to late stages of growth time course. For cell expansion markers, MdEXP3 was upregulated as the cells expanded, while MdARF106 was expressed in both the cell division and expansion stages. Meanwhile, the ripening related gene MdACO1 was expectedly expressed only during the ending stages associated with ripening. Interestingly, the cell measurements taken regularly from each cultivar throughout the same eperimental timespan showed that cell sizes were unaltered and remained constant from initial pollination at the zeroth Days After Pollination (DAP), to ripening at 120 Days After Full Bloom (DAFB).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cell division controls final fruit size in three apple (Malus x domestica) cultivars

Karim¹,

Allan²,

Schaffer³

et al. 2022

Preprint

View full text Add to dashboard Cite

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Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection

Liang

et al. 2024

Horticultural Plant Journal

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MdAIL5 overexpression promotes apple adventitious shoot regeneration by regulating hormone signaling and activating the expression of shoot development-related genes

Liu,

Yang,

Yan

et al. 2023

Horticulture Research

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Adventitious shoot (AS) regeneration is a significant factor in the genetic transformation of horticultural plants. It is also a noteworthy approach for their vegetative propagation. AS regeneration remains highly dependent on the genotype or maturity of explants. We here found that the AS regeneration abilities of apple leaves were positively correlated with MdAIL5 expression. MdAIL5 overexpression dramatically increased the AS regeneration efficiency. Notably, MdAIL5 overexpression could restore the AS formation ability of explants to a certain extent, which was lost with an increase in maturity. Endogenous hormone detection revealed that MdAIL5 overexpression changed the contents of auxin, cytokinin (CK), and other hormones in apple leaves. Transcriptome analysis revealed that many genes related to auxin, CK, and brassinolide signaling pathways were significantly and differentially expressed between MdAIL5-overexpressing transgenic apple and wild-type apple plants. Yeast one-hybrid assays, electrophoretic mobility shift assay, and dual-luciferase reporter assay revealed that MdAIL5 directly binds to MdARF9 and MdHB14 promoters and positively affects their expression. We here established a model of MdAIL5 regulating AS formation, which acts as a theoretical basis for facilitating genotype- or explant maturity-independent AS regeneration in the future.

show abstract

Genome-wide identification and characterization of AINTEGUMENTA-LIKE (AIL) family genes in apple (Malus domestica Borkh.)

Cited by 10 publications

References 59 publications

Cell division controls final fruit size in three apple (Malus x domestica) cultivars

Cell division controls final fruit size in three apple (Malus x domestica) cultivars

Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection

MdAIL5 overexpression promotes apple adventitious shoot regeneration by regulating hormone signaling and activating the expression of shoot development-related genes

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