Genome-Wide Identification and Characterization of Fusarium graminearum-Responsive lncRNAs in Triticum aestivum

Abstract

Although the war between wheat and Fusarium has been widely investigated for years, long noncoding RNAs (lncRNAs), which have been proven to regulate important processes in the development and stress responses of plants, are still poorly known in wheat against Fusarium. Herein, we systematically reveal the roles of wheat lncRNAs in the process of Fusarium graminearum infection by high-throughput RNA sequencing. Well over 4130 of the total 4276 differentially expressed lncRNAs were already specifically expressed at 12 h postinoculation (hpi), but only 89 of these were specifically expressed at 24 hpi, indicating that the initial stage was the crucial stage for lncRNA-mediated gene regulation of wheat defense against F. graminearum. Target analysis showed the lncRNAs participated in various biological stress processes and had exclusive regulation models at different infection stages. Further H2O2 accumulation and protein ubiquitination assays supported this idea. Moreover, two lncRNAs (XLOC_302848 and XLOC_321638) were identified as Fusarium seedling blight resistance candidates by lncRNA-target expression pattern validation, and two lncRNAs (XLOC_113815, XLOC_123624) were Fusarium head blight resistance potential regulators by cross-validating the RNAseq data with the refined meta-QTL of wheat FHB resistance. These findings extend our knowledge on wheat lncRNAs response to F. graminearum attack and provide new insights for the functional and molecular research of future interactions between wheat and Fusarium.

Genome-wide identification of the CYP82 gene family in cucumber and functional characterization of CsCYP82D102 in regulating resistance to powdery mildew

“…The recommended conditions for PCR were used as follows: 95 °C for 10 min, followed by 40 cycles of 95 °C for 30 s, 58 °C for 30 s, and 72 °C for 30 s. All reactions were performed in triplicate for each sample. The relative expression analysis of each gene were calculated using the 2 −ΔΔCT method ( Duan et al, 2020 ).…”

Deoxynivalenol: Emerging Toxic Mechanisms and Control Strategies, Current and Future Perspectives

“…The mechanism involved in mycotoxin toxicity includes lncRNA. , Several studies have found that exposure to aflatoxin B 1 (AFB 1 ) changes the expression of lncRNA and promotes the development of hepatotoxicity and cancer. − OTA induces the overexpression of lncRNA in renal cell carcinoma and is related to cell death pathways and metabolism . The involvement of lncRNA in the regulation of mycotoxins is expected to become the focus of research because two lncRNAs (XLOC_302848 and XLOC_321638) are potential regulators of Fusarium head blight resistance . A recent study found that an lncRNA, lncRsp1, negatively regulated the expression of DON biosynthetic genes, TRI4 , TRI5 , TRI6 , and TRI13 , and the production of DON …”

“…34 The involvement of lncRNA in the regulation of mycotoxins is expected to become the focus of research because two lncRNAs (XLOC_302848 and XLOC_321638) are potential regulators of Fusarium head blight resistance. 35 A recent study found that an lncRNA, lncRsp1, negatively regulated the expression of DON biosynthetic genes, TRI4, TRI5, TRI6, and TRI13, and the production of DON. 13 As a class of endogenous noncoding small molecules, miRNA usually exists in animals, plants, and viruses and participates in the regulation of many physiological diseases, including apoptosis, differentiation, necrosis, and inflammation.…”

RNA-Seq Implies Divergent Regulation Patterns of LincRNA on Spermatogenesis and Testis Growth in Goats

“…LincRNA-Cox2 increases the expression of the neighboring gene prostaglandin-endoperoxide synthase (Ptgs2) through an enhancer RNA mechanism, contributing to prostaglandin biosynthesis [68]. Most studies use 10 kb or 100 kb upstream and downstream as the estimated range of lincRNA cis-target genes [69,70]. In addition, some lncRNAs bind with mRNAs to form dimers, thereby reducing mRNA expression [67].…”