Genome-Wide Identification and Characterization of Heat-Shock Transcription Factors in Rubber Tree

Li, Yán; Yu, Wencai; Chen, Yueyi; Yang, Shuguang; Wu, Shan; Chao, Jinquan; Wang, Xinlong; Tian, Weimin

doi:10.3390/f10121157

Cited by 9 publications

(8 citation statements)

References 38 publications

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“…For example, AtHSFA7b positively regulates salt tolerance of chrysanthemum and Arabidopsis (Li et al, 2018;Zang et al, 2019). HbHsfA7a was significantly upregulated under low-temperature stress in the other hardy rubber (Li et al, 2019c). Promoter analysis also showed that A1 and A7 may participate in the regulation of development and abiotic stresses in B. juncea.…”

Section: Analysis Of the Expression Of Bjuhsf Genesmentioning

confidence: 96%

Genome-Wide Analysis of the Heat Shock Transcription Factor Gene Family in Brassica juncea: Structure, Evolution, and Expression Profiles

Xie

et al. 2020

DNA and Cell Biology

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Heat shock transcription factor (HSF) is ubiquitous in the whole biological world and plays an important role in regulating growth and development and responses to environment stress. In this study, a total of 60 HSF transcription factors in Brassica juncea genome were identified and analyzed. Phylogenetic analysis showed that HSF genes were divided into three groups namely: A, B, and C, of which group A was further divided into nine subgroups (A1-A9). The analysis of gene structure and conserved motifs showed that some homologous genes are highly conserved. There was strong conservative microcollinearity among Brassica rapa, B. juncea, and Brassica oleracea, which provides a basis for studying the replication of gene families. Moreover, the results revealed that the promoter regions of BjuHSF genes were rich in cis-elements related to growth and development, hormone signal, and stress response. The prediction of protein interaction results showed that HSFs could interact with multiple transcription factors and proteins in the genome, while functional annotation revealed that BjuHSF genes were involved in many biological processes. The expression patterns of BjuHSF genes were analyzed by qPCR, and the results showed that these genes were closely linked to stress response, hormones, and development process. These results are a foundation for further analysis of the regulation mechanism of HSF gene family.

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Section: Analysis Of the Expression Of Bjuhsf Genesmentioning

confidence: 96%

Genome-Wide Analysis of the Heat Shock Transcription Factor Gene Family in Brassica juncea: Structure, Evolution, and Expression Profiles

Xie

et al. 2020

DNA and Cell Biology

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“…To analyse gene expression in four cold-tolerant rubber tree clones and four coldsensitive rubber tree clones, the treatment of the grafted plantlets was performed under cold stress in accordance with a published protocol [26]. Bark samples were collected from five plantlets of each rubber tree clone at 0 h, 4 h, 8 h, and 24 h of treatment and mixed as one sample.…”

Section: Stress Treatmentsmentioning

confidence: 99%

“…Quantitative real-time PCR (qPCR) was carried out with 2 ×TB Green TM Premix Ex Taq TM II (TliRNaseH Plus) (TaKaRa, Shiga, Japan) through the CFX384 Real-Time System (Bio-Rad, Herakles, CA, USA) according to the described protocol [26]. Each sample used three biological replicates, and each biological replicate used three technical replicates for qPCR analysis.…”

Section: Quantitative Expression Analysismentioning

confidence: 99%

“…The encoding sequences of HbICE1, HbICE2, HbICE3, HbICE4, and HbICE5 were constructed into the pCAMBIA1302 vector, respectively. HbICE1::GFP, HbICE2::GFP, HbICE3::GFP, HbICE4::GFP, and HbICE5::GFP recombinant plasmids and the empty pCAMBIA1302 vector were introduced into living onion epidermal cells by Agrobacterium GV3101 as previously described [26]. The localization of fusion proteins analysis was carried out using a laser confocal scanning microscope (Zeiss LSM 800, Germany) after 24 h incubation in the dark.…”

Section: Subcellular Localization Analysismentioning

confidence: 99%

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Functional Identification of ICE Transcription Factors in Rubber Tree

Quan

Yang

et al. 2022

Forests

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ICE (inducer of CBF expression) is a positive regulator of cold signaling pathway in plants. Identification of ICE transcription factors is important for the sustainable development of the natural rubber planting industry in nontraditional regions where sudden cold waves often occur. In this study, five ICE genes were isolated from genome of rubber tree (Hevea brasiliensis Muell. Arg.) for analysing tolerance to cold stress. They shared an ICE-specific region in the highly conserved bHLH-ZIP domain and were localized in the nucleus. The HbICEs were different in transcript abundance and expression patterns in response to cold and drought stresses and among different rubber tree clones. Generally, the expression level of HbICEs was significantly higher in the cold-tolerant rubber tree clones than that in the cold-sensitive rubber tree clones. Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco, which showed a significant increase in chlorophyll content and decrease in relative water content and conductivity at the early stage of cold stress in comparison with wild-type plants. Furthermore, overexpression of HbICE2 and HbICE4, but also HbICE1 enhanced drought tolerance in transgenic Arabidopsis. The cold tolerance of rubber tree clones is positively controlled by the expression level of HbICE1, HbICE2, and HbICE4.

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“…After 48 h, the plants were divided into five groups, each group containing 105 plantlets, that were exposed to different treatment conditions. According to our recent study ( Li et al, 2019 ), two groups of seedlings were transferred to 4 °C and 40 °C growth chambers for low- and high temperature treatments, respectively. One group of plantlets was removed from the nutritious bags and cultivatable soil, and bare roots were placed in flowerpots for drought stress treatment.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide identification of the rubber tree superoxide dismutase (SOD) gene family and analysis of its expression under abiotic stress

GuangHong

Chao

et al. 2022

PeerJ

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Background The rubber tree (Hevea brasiliensis) is the only species capable of producing high-quality natural rubber for commercial use, and is often subjected to various abiotic stresses in non-traditional rubber plantation areas. Superoxide dismutase (SOD) is a vital metalloenzyme translated by a SOD gene family member and acts as a first-line of protection in plant cells by catalysing the disproportionation of reactive oxygen species (ROS) to produce H2O2 and O2. However, the SOD gene family is not reported in rubber trees. Methods Here, we used hidden markov model (HMM) and BLASTP methods to identify SOD genes in the H. brasiliensis genome. Phylogenetic tree, conserved motifs, gene structures, cis elements, and gene ontology annotation (GO) analyses were performed using MEGA 6.0, MEME, TBtools, PlantCARE, and eggNOG database, respectively. HbSOD gene expression profiles were analysed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results We identified nine HbSOD genes in the rubber tree genome, including five HbCSDs, two HbFSDs, and two HbMSDs. Phylogenetic relationship analysis classified the SOD proteins from the rubber tree and other related species into three subfamilies. The results of gene structure and conserved motif analysis illustrated that most HbSOD genes have similar exon-intron numbers and conserved motifs in the same evolutionary branch. Five hormone-related, four stress-related, and light-responsive elements were detected in the HbSODs’ promoters. HbSODs were expressed in different tissues, gradually increased with leaf development, and were abundantly expressed in mature leaves. HbCSD2 and HbCSD4 was significantly upregulated under low and high temperatures, and salt stress, except for HbCSD2, by heat. Furthermore, most HbSOD genes were significantly upregulated by drought, except HbMSD2. These findings imply that these genes may play vital roles in rubber tree stress resistance. Our results provide a basis for further studies on the functions of HbSOD genes in rubber trees and stress response mechanisms.

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Genome-Wide Identification and Characterization of Heat-Shock Transcription Factors in Rubber Tree

Cited by 9 publications

References 38 publications

Genome-Wide Analysis of the Heat Shock Transcription Factor Gene Family in Brassica juncea: Structure, Evolution, and Expression Profiles

Genome-Wide Analysis of the Heat Shock Transcription Factor Gene Family in Brassica juncea: Structure, Evolution, and Expression Profiles

Functional Identification of ICE Transcription Factors in Rubber Tree

Genome-wide identification of the rubber tree superoxide dismutase (SOD) gene family and analysis of its expression under abiotic stress

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