Genome-Wide Identification and Characterization of Aquaporins and Their Role in the Flower Opening Processes in Carnation (Dianthus caryophyllus)

Kong, Weilong; Bendahmane, Mohammed; Fu, Xiaopeng

doi:10.3390/molecules23081895

Cited by 24 publications

(40 citation statements)

References 81 publications

(153 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…That includes flowers, but even more during the initial phases of seed germination, for which the TIP3 subfamily is showed to be specifically and/or strongly expressed [62,[76][77][78]. These results suggested that TIP3's gene function is not strictly conserved in plants, and this assertion was also reported in Jatropha curcas L. [18], banana [79] and carnation [80]. It is reasonable to hypothesize that other AQP proteins may replace this TIP3 function in flowers in several plant species, including olive trees.…”

Section: Transcriptional Profiling Of Olive Oleuraqpsmentioning

confidence: 68%

Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.)

Faize

Fumanal

Luque

et al. 2020

IJMS

View full text Add to dashboard Cite

Cellular aquaporin water channels (AQPs) constitute a large family of transmembrane proteins present throughout all kingdoms of life, playing important roles in the uptake of water and many solutes across the membranes. In olive trees, AQP diversity, protein features and their biological functions are still largely unknown. This study focuses on the structure and functional and evolution diversity of AQP subfamilies in two olive trees, the wild species Olea europaea var. sylvestris (OeuAQPs) and the domesticated species Olea europaea cv. Picual (OleurAQPs), and describes their involvement in different physiological processes of early plantlet development and in biotic and abiotic stress tolerance in the domesticated species. A scan of genomes from the wild and domesticated olive species revealed the presence of 52 and 79 genes encoding full-length AQP sequences, respectively. Cross-genera phylogenetic analysis with orthologous clustered OleaAQPs into five established subfamilies: PIP, TIP, NIP, SIP, and XIP. Subsequently, gene structures, protein motifs, substrate specificities and cellular localizations of the full length OleaAQPs were predicted. Functional prediction based on the NPA motif, ar/R selectivity filter, Froger’s and specificity-determining positions suggested differences in substrate specificities of Olea AQPs. Expression analysis of the OleurAQP genes indicates that some genes are tissue-specific, whereas few others show differential expressions at different developmental stages and in response to various biotic and abiotic stresses. The current study presents the first detailed genome-wide analysis of the AQP gene family in olive trees and it provides valuable information for further functional analysis to infer the role of AQP in the adaptation of olive trees in diverse environmental conditions in order to help the genetic improvement of domesticated olive trees.

show abstract

Section: Transcriptional Profiling Of Olive Oleuraqpsmentioning

confidence: 68%

Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.)

Faize

Fumanal

Luque

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Thereof, HbXIP2;1 is expressed in all vegetative tissues. This rather low, but general expression in the plant body seems common to many analyzed XIPs (Bienert et al., ; Kong, Bendahmane, & Fu, ). Interestingly, HbXIP2;1 expression is upregulated specifically during latex tapping and by ethylene treatments.…”

Section: Introductionmentioning

confidence: 82%

“…Thereof, HbXIP2;1 is expressed in all vegetative tissues. This rather low, but general expression in the plant body seems common to many analyzed XIPs (Bienert et al, 2011;Kong, Bendahmane, & Fu, 2018).…”

Section: Introductionmentioning

confidence: 84%

Overexpression of X Intrinsic Protein 1;1 in Nicotiana tabacum and Arabidopsis reduces boron allocation to shoot sink tissues

et al. 2019

View full text Add to dashboard Cite

Major Intrinsic Proteins ( MIP ) are a family of channels facilitating the diffusion of water and/or small solutes across cellular membranes. X Intrinsic Proteins ( XIP ) form the least characterized MIP subfamily in vascular plants. XIP s are mostly impermeable to water but facilitate the diffusion of hydrogen peroxide, urea and boric acid when expressed in heterologous expression systems. However, their transport capabilities in planta and their impact on plant physiology are still unknown. Here, we demonstrated that overexpression of Nt XIP 1;1 in Nicotiana tabacum by the En2p PMA 4 or the 35S Ca MV promoter and in Arabidopsis, which does not contain any XIP gene, by the 35S Ca MV promoter, resulted in boron (B)‐deficiency symptoms such as death of the shoot apical meristem, infertile flowers, and puckered leaves. Leaf B concentrations in symptomatic tissues and B xylem sap concentrations were lower in the overexpressors than in control plants. Importantly, expression of Nt XIP 1;1 under the control of the At NIP 5;1 promoter complemented the B deficiency phenotype of the Atnip5;1 knockout mutant, defining its ability to act as a boric acid channel in planta . Protein quantification analysis revealed that Nt XIP 1;1 was predominantly expressed in young B‐demanding tissues and induced under B‐deficient conditions. Our results strongly suggest that Nt XIP 1;1 plays a role in B homeostasis and its tissue‐specific expression critically contributes to the distribution of B within tobacco.

show abstract

“…Q30, GC-content, and sequence duplication level of the clean data were calculated, respectively. HISAT2 software was used to map all clean reads to the reference genome of RPY geng (unpublished data) [51,52], and the mapped reads were assembled and quantified using StringTie software [53]. Gene expression levels were estimated by fragments per kilobase of transcript per million fragments mapped reads (FPKM) method [54,55].…”

Section: Transcriptome Sequencing and Rna-seq Analysismentioning

confidence: 99%

Integrated RNA-seq Analysis and Meta-QTLs Mapping Provide Insights into Cold Stress Response in Rice Seedling Roots

Kong

Zhang

Qiang

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Rice (Oryza sativa L.) is a widely cultivated food crop around the world, especially in Asia. However, rice seedlings often suffer from cold stress, which affects their growth and yield. Here, RNA-seq analysis and Meta-QTLs mapping were performed to understand the molecular mechanisms underlying cold tolerance in the roots of 14-day-old seedlings of rice (RPY geng, cold-tolerant genotype). A total of 4779 of the differentially expressed genes (DEGs) were identified, including 2457 up-regulated and 2322 down-regulated DEGs. The GO, COG, KEEG, and Mapman enrichment results of DEGs revealed that DEGs are mainly involved in carbohydrate transport and metabolism, signal transduction mechanisms (plant hormone signal transduction), biosynthesis, transport and catabolism of secondary metabolites (phenylpropanoid biosynthesis), defense mechanisms, and large enzyme families mechanisms. Notably, the AP2/ERF-ERF, NAC, WRKY, MYB, C2H2, and bHLH transcription factors participated in rice’s cold–stress response and tolerance. On the other hand, we mapped the identified DEGs to 44 published cold–stress-related genes and 41 cold-tolerant Meta-QTLs regions. Of them, 12 DEGs were the published cold–stress-related genes and 418 DEGs fell into the cold-tolerant Meta-QTLs regions. In this study, the identified DEGs and the putative molecular regulatory network can provide insights for understanding the mechanism of cold stress tolerance in rice. In addition, DEGs in KEGG term-enriched terms or cold-tolerant Meta-QTLs will help to secure key candidate genes for further functional studies on the molecular mechanism of cold stress response in rice.

show abstract

Genome-Wide Identification and Characterization of Aquaporins and Their Role in the Flower Opening Processes in Carnation (Dianthus caryophyllus)

Cited by 24 publications

References 81 publications

Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.)

Genome Wild Analysis and Molecular Understanding of the Aquaporin Diversity in Olive Trees (Olea Europaea L.)

Overexpression of X Intrinsic Protein 1;1 in Nicotiana tabacum and Arabidopsis reduces boron allocation to shoot sink tissues

Integrated RNA-seq Analysis and Meta-QTLs Mapping Provide Insights into Cold Stress Response in Rice Seedling Roots

Contact Info

Product

Resources

About