Genome-Wide Identification and Analysis of NBS-LRR-Encoding Genes in Mungbean (Vigna radiata L. Wilczek) and Their Expression in Two Wild Non-progenitors Reveal Their Role in MYMIV Resistance

Purwar, Shalini; Singh, C. M.; Kumar, Mukul; Singh, Akhilesh Kumar; Pratap, Aditya; Singh, Poornima; Gore, Padmavati G.; Singh, Narendra Pratap

doi:10.1007/s00344-023-10948-7

Cited by 5 publications

(5 citation statements)

References 49 publications

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“…The TIR-NBS-LRR class of disease resistance protein family (chr. 4 and 9) was found to regulate YMD resistance in mungbean as also reported by Purwar et al (2023) . Depuydt & Vandepoele (2021) also reported the involvement of this gene in a variety of developmental processes and molecular responses, including defense responses.…”

Section: Discussionsupporting

confidence: 73%

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Genome-wide association studies for earliness, MYMIV resistance, and other associated traits in mungbean (Vigna radiata L. Wilczek) using genotyping by sequencing approach

Kohli,

Bansal,

Mishra

et al. 2024

PeerJ

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Yellow mosaic disease (YMD) remains a major constraint in mungbean (Vigna radiata (L.)) production; while short-duration genotypes offer multiple crop cycles per year and help in escaping terminal heat stress, especially during summer cultivation. A comprehensive genotyping by sequencing (GBS)-based genome-wide association studies (GWAS) analysis was conducted using 132 diverse mungbean genotypes for traits like flowering time, YMD resistance, soil plant analysis development (SPAD) value, trichome density, and leaf area. The frequency distribution revealed a wide range of values for all the traits. GBS studies identified 31,953 high-quality single nucleotide polymorphism (SNPs) across all 11 mungbean chromosomes and were used for GWAS. Structure analysis revealed the presence of two genetically distinct populations based on ΔK. The linkage disequilibrium (LD) varied throughout the chromosomes and at r2 = 0.2, the mean LD decay was estimated as 39.59 kb. Two statistical models, mixed linear model (MLM) and Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK) identified 44 shared SNPs linked with various candidate genes. Notable candidate genes identified include FPA for flowering time (VRADI10G01470; chr. 10), TIR-NBS-LRR for mungbean yellow mosaic India virus (MYMIV) resistance (VRADI09G06940; chr. 9), E3 ubiquitin-protein ligase RIE1 for SPAD value (VRADI07G28100; chr. 11), WRKY family transcription factor for leaf area (VRADI03G06560; chr. 3), and LOB domain-containing protein 21 for trichomes (VRADI06G04290; chr. 6). In-silico validation of candidate genes was done through digital gene expression analysis using Arabidopsis orthologous (compared with Vigna radiata genome). The findings provided valuable insight for marker-assisted breeding aiming for the development of YMD-resistant and early-maturing mungbean varieties.

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Section: Discussionsupporting

confidence: 73%

“…9) as having a role in YMD resistance. Similarly, Purwar et al (2023) also reported the role of NBS-LRR-encoding genes in the imposition of YMD resistance in mungbean and two wild non-progenitors. The TIR-NBS-LRR class of disease resistance protein family (chr.…”

Section: Discussionmentioning

confidence: 88%

Genome-wide association studies for earliness, MYMIV resistance, and other associated traits in mungbean (Vigna radiata L. Wilczek) using genotyping by sequencing approach

Kohli,

Bansal,

Mishra

et al. 2024

PeerJ

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“…The interplay between pathogen sensing signals could lead to the significant interventions (Spoel & Dong, 2012; Zuluaga et al, 2016). The R‐proteins are well known to activate during the pathogen attack in the genotypes (Dasgupta et al, 2021; Kundu et al, 2019; Purwar et al, 2023). The significant expression of NB‐LRR ‐gene, PR‐1 , DEF and CYR‐1 in the resistant genotypes upon MYMIV infection indicated the activation of defence mechanism.…”

Section: Discussionmentioning

confidence: 99%

Identification of potential sources of mungbean yellow mosaic India virus resistance in black gram (Vigna mungo) and expression of antioxidants and R‐genes modulating resistance response in cultivated and its two wild relatives

Tripathi¹,

Singh²,

Kumar³

et al. 2023

Plant Breeding

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Black gram is one of the most important short duration grain legume, which contributes significantly towards nutritional security and environmental sustainability. The virus specific primers confirms the presence of mungbean yellow mosaic India virus (MYMIV) in representative samples. A total of 27 cultivated and two wild species were found as highly resistant (HR) to MYMIV and validated through molecular markers. The start codon target (SCoT) markers analysis revealed that the SCoT loci, namely, SCoT‐4 (2200 bp), SCot‐9 (1150/ 1200 bp), SCoT‐15 (1150/1100 bp), SCoT‐16 (700 bp), SCoT‐24 (2500 bp), SCoT‐25 (700 bp), SCoT‐33 (900/1000 bp), and SCoT‐34 (600 bp), were found unique, able to distinguish HR and highly susceptible (HS) genotypes. Biochemical characterization and gene expression profiling revealed the higher expression of antioxidants and R‐genes just after pathogen inoculation indicated the activation of defence mechanism in both cultivated and its wild relatives, which modulates the resistant responses in cultivated and wild accessions. These information will be really helpful in accelerating resistance breeding in black gram.

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“…Crop wild relatives (CWRs) also play a very important role in crop improvement [ 77 ]. Pal et al [ 78 ] created crosses of ricebean × urdbean.…”

Section: Discussionmentioning

confidence: 99%

Screening of Comprehensive Panel of Cultivated and Wild Vigna Species for Resistance to Pulse Beetle, Callosobruchus chinensis L.

Sahu¹,

Singh²,

Pandey³

et al. 2023

Biology

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Pulses are a key source of dietary proteins in human nutrition. Despite several efforts to increase the production, various constraints, such as biotic and abiotic factors, threaten pulse production by various means. Bruchids (Callosobruchus spp.) are the serious issue of concern, particularly in storage conditions. Understanding host–plant resistance at morphological, biochemical and molecular levels is the best way to minimize yield losses. The 117 mungbean (Vigna radiata L. Wilczek) genotypes, including endemic wild relatives, were screened for resistance against Callosobruchus chinensis; among them, two genotypes, PRR 2008-2 and PRR 2008-2-sel, which belong to V. umbellata (Thumb.), were identified as highly resistant. The expression of antioxidants in susceptible and resistant genotypes revealed that the activity of phenylalanine ammonia lyase (PAL) was upregulated in the highly resistant wild Vigna species and lower in the cultivated susceptible genotypes, along with other biomarkers. Further, the SCoT-based genotyping revealed SCoT-30 (200 bp), SCoT-31 (1200 bp) and SCoT-32 (300 bp) as unique amplicons, which might be useful for developing the novel ricebean-based SCAR markers to accelerate the molecular breeding programme.

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Genome-Wide Identification and Analysis of NBS-LRR-Encoding Genes in Mungbean (Vigna radiata L. Wilczek) and Their Expression in Two Wild Non-progenitors Reveal Their Role in MYMIV Resistance

Cited by 5 publications

References 49 publications

Genome-wide association studies for earliness, MYMIV resistance, and other associated traits in mungbean (Vigna radiata L. Wilczek) using genotyping by sequencing approach

Genome-wide association studies for earliness, MYMIV resistance, and other associated traits in mungbean (Vigna radiata L. Wilczek) using genotyping by sequencing approach

Identification of potential sources of mungbean yellow mosaic India virus resistance in black gram (Vigna mungo) and expression of antioxidants and R‐genes modulating resistance response in cultivated and its two wild relatives

Screening of Comprehensive Panel of Cultivated and Wild Vigna Species for Resistance to Pulse Beetle, Callosobruchus chinensis L.

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