Genome-Wide Identification and Analysis of the Type-B Authentic Response Regulator Gene Family in Peach (<b><i>Prunus persica</i></b>)

Abstract

The type-B authentic response regulator (ARR-B) family members serve as DNA-binding transcriptional regulators, whose activities are probably regulated by phosphorylation/dephosphorylation, resulting in the rapid induction of type-A ARR genes. Type-B ARRs are believed to be involved in many biological processes, including cytokinin signaling, plant growth, and stress responses through a chaperone or by isomerization of proline residues during protein folding. The public availability of complete peach genome sequences allows the identification of 23 ARR-B genes by HMMER and blast analysis. Scaffold locations of these genes in the peach genome were determined, and the protein domain and motif organization of peach type-B ARRs were analyzed. The phylogenetic relationships between peach type-B ARRs were also assessed. The expression profiles of peach ARR-B genes revealed that most of the type-B ARRs showed high expression levels in tissues undergoing rapid cell division and may engage more cytokinins, like half-opened flowers, fruits at expansion stages, and young leaves. These findings not only contribute to a better understanding of the complex regulation of the peach ARR-B gene family, but also provide valuable information for future research in peach functional genomics.

The molecular characteristics of soybean ARR-B transcription factors

“…This paper has systematically analyzed the type-B ARR gene family in soybean, assigned the GmARR-B genes family. The results revealed that theGmARR-B genes family consisted of 31 gene members in soybean, which was largely more than those identified in other species, such as Arabidopsis (12) (Ramírez-Carvajal et al, 2008), rice (13) (Schaller et al, 2007), pear (23) (Ni et al, 2017), and peach (11) (Zeng et al, 2017). In previous studies on TCS system of soybean, 15 type-B response regulatory factors were also found, which were inconsistent with the results of this experiment.…”

“…Because of their apparent multifunctionality, type-B ARR genes are promising targets for developing crop varieties that are better adapted to abiotic stresses. Type-B ARRs have been identified and applied to enhance abiotic stress resistance in many plants, including Arabidopsis thaliana, Populus, rice, pear, and peach (Schaller et al, 2008;Yu et al, 2020;Huang et al, 2019;Ni et al, 2017;Zeng et al, 2017). Here, we also performed a genome-wide search of type-B ARR gene family members in soybean.…”

Seed Priming: A Feasible Strategy to Enhance Drought Tolerance in Crop Plants

“…The regulation of gene expression at the transcriptome level is influenced by transcription factors (TFs) [122]. Several TF genes were identified from different families to change the network of gene expression for drought adaptation in plants, such as MYB, MYC, NAC, bZIP, HD-ZIP, and DERB, and these have gained attention due to their magnificent role in drought tolerance through ABA-independent or ABA-dependent pathways [123,124]. Overexpression of AtWRKY57 hoists the ABA level and induces drought stress tolerance in Arabidopsis.…”

Drought stress revealed physiological, biochemical and gene-expressional variations in ‘Yoshihime’ peach (Prunus Persica L) cultivar

“…Under decreased relative water content (RWC), the signal transduction activates the hydrogen pump ATPase (H + ATPase) proteins on the plasma membrane of root hairs, which incites the biosynthesis of unequivocal osmolytes to adjust water balance. Besides, various transcription factors (TFs) gene families, including WRKY TF, ethylene-responsive factor (ERF), dehydrationresponsive element-binding (DREB), myeloblastosis, myelocytomatosis oncogene cellular (MYC), basic helix-loophelix TF, and basic leucine zipper (bZIP) are implicated to trigger the specific genes to generate the requisite defense responses in plants (Abuzar et al 2016;Zeng et al 2017). Being largest families of TFs, WRKY proteins have been testified repeatedly against drought stress, for example, the overexpression of AtWRKY57 has hoisted the ABA level and promote the drought tolerance in Arabidopsis (Ren et al 2010).…”