Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (Solanum tuberosum L.)

Shang, Yutong; Luo, Xiaobo; Zhang, Heng; Chen, Mingjun; Yin, Wang; Cao, Zhenju; Deng, Renju; Li, Yan; Li, Fēi

doi:10.3390/agronomy13010093

Cited by 4 publications

(1 citation statement)

References 54 publications

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“…The MAPK signaling pathway has important effects on cell growth, stress, and the immune response [29]. The MAPK signaling pathway contributes to the response to low temperatures in both animals and plants, such as Larimichthys crocea [30], Solanum tuberosum L. [31], and the pearl gentian grouper (E. lanceolatus♂× E. fuscoguttatus♀) [32]. In humans, sperm motility is regulated via the MAPK signaling pathway [33].…”

Section: Differential Expression Between Fresh and Frozen Sperm Of E ...mentioning

confidence: 99%

Effects of Long-Term Cryopreservation on the Transcriptomes of Giant Grouper Sperm

Ding,

Tian,

Qiu

et al. 2024

Genes

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The giant grouper fish (Epinephelus lanceolatus), one of the largest and rarest groupers, is a fast-growing economic fish. Grouper sperm is often used for cross-breeding with other fish and therefore sperm cryopreservation is important. However, freezing damage cannot be avoided. Herein, we performed a transcriptome analysis to compare fresh and frozen sperm of the giant grouper with frozen storage times of 0, 23, 49, and 61 months. In total, 1911 differentially expressed genes (DEGs), including 91 in El-0-vs-El-23 (40 upregulated and 51 downregulated), 251 in El-0-vs-El-49 (152 upregulated and 69 downregulated), and 1569 in El-0-vs-El-61 (984 upregulated and 585 downregulated), were obtained in the giant grouper sperm. DEGs were significantly increased at 61 months of cryopreservation (p < 0.05). GO and KEGG enrichment analyses of the DEGs revealed significant enrichment in the pilus assembly, metabolic process, MAPK signaling pathway, apoptosis, and P53 signaling pathway. Time-series expression profiling of the DEGs showed that consistently upregulated modules were also significantly enriched in signaling pathways associated with apoptosis. Four genes, scarb1, odf3, exoc8, and atp5f1d, were associated with mitochondria and flagella in a weighted correlation network analysis. These genes may play an important role in the response to sperm freezing. The experimental results show that long-term cryopreservation results in freezing damage to the giant grouper sperm. This study provides rich data for studies of the mechanism underlying frozen fish sperm damage as well as a technical reference and evaluation index for the long-term cryopreservation of fish sperm.

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Section: Differential Expression Between Fresh and Frozen Sperm Of E ...mentioning

confidence: 99%

Effects of Long-Term Cryopreservation on the Transcriptomes of Giant Grouper Sperm

Ding,

Tian,

Qiu

et al. 2024

Genes

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A close-up of regulatory networks and signaling pathways of MKK5 in biotic and abiotic stresses

Movahedi,

Hwarari,

Dzinyela

et al. 2024

Critical Reviews in Biotechnology

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Molecular traits of MAPK kinases and the regulatory mechanism of GhMAPKK5 alleviating drought/salt stress in cotton

Ding,

Li,

Wang

et al. 2024

Plant Physiology

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Mitogen-activated protein kinase kinases (MAPKKs) play a critical role in the mitogen-activated protein kinase (MAPK) signaling pathway, transducing external stimuli into intracellular responses and enabling plant adaptation to environmental challenges. Most research has focused on the model plant Arabidopsis (Arabidopsis thaliana). The systematic analysis and characterization of MAPKK genes across different plant species, particularly in cotton (Gossypium hirsutum), are somewhat limited. Here, we identified MAPKK family members from 66 different species, which clustered into 5 different sub-groups, and MAPKKs from four cotton species clustered together. Through further bioinformatic and expression analysis, GhMAPKK5 was identified as the most responsive MAPKK member to salt and drought stress among the 23 MAPKKs identified in Gossypium hirsutum. Silencing GhMAPKK5 in cotton through virus-induced gene silencing (VIGS) led to quicker wilting under salt and drought conditions, while overexpressing GhMAPKK5 in Arabidopsis enhanced root growth and seed germination under these stresses, demonstrating GhMAPKK5's positive role in stress tolerance. Transcriptomics and Yeast-Two-Hybrid assays revealed a MAPK cascade signal module comprising GhMEKK (Mitogen-activated protein kinase kinase kinases)3/8/31-GhMAPKK5-GhMAPK11/23. This signaling cascade may play a role in managing drought and salt stress by regulating transcription factor genes, such as WRKYs, which are involved in the biosynthesis and transport pathways of ABA, proline, and RALF. This study is highly important for further understanding the regulatory mechanism of MAPKK in cotton, contributing to its stress tolerance and offering potential in targets for genetic enhancement.

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Genome-Wide Identification and Analysis of the MAPK and MAPKK Gene Families in Potato (Solanum tuberosum L.)

Cited by 4 publications

References 54 publications

Effects of Long-Term Cryopreservation on the Transcriptomes of Giant Grouper Sperm

Effects of Long-Term Cryopreservation on the Transcriptomes of Giant Grouper Sperm

A close-up of regulatory networks and signaling pathways of MKK5 in biotic and abiotic stresses

Molecular traits of MAPK kinases and the regulatory mechanism of GhMAPKK5 alleviating drought/salt stress in cotton

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