Genome-wide identification and analysis of the heat shock transcription factor family in moso bamboo (Phyllostachys edulis)

Abstract

Heat shock transcription factors (HSFs) are central elements in the regulatory network that controls plant heat stress response. They are involved in multiple transcriptional regulatory pathways and play important roles in heat stress signaling and responses to a variety of other stresses. We identified 41 members of the HSF gene family in moso bamboo, which were distributed non-uniformly across its 19 chromosomes. Phylogenetic analysis showed that the moso bamboo HSF genes could be divided into three major subfamilies; HSFs from the same subfamily shared relatively conserved gene structures and sequences and encoded similar amino acids. All HSF genes contained HSF signature domains. Subcellular localization prediction indicated that about 80% of the HSF proteins were located in the nucleus, consistent with the results of GO enrichment analysis. A large number of stress response–associated cis-regulatory elements were identified in the HSF upstream promoter sequences. Synteny analysis indicated that the HSFs in the moso bamboo genome had greater collinearity with those of rice and maize than with those of Arabidopsis and pepper. Numerous segmental duplicates were found in the moso bamboo HSF gene family. Transcriptome data indicated that the expression of a number of PeHsfs differed in response to exogenous gibberellin (GA) and naphthalene acetic acid (NAA). A number of HSF genes were highly expressed in the panicles and in young shoots, suggesting that they may have functions in reproductive growth and the early development of rapidly-growing shoots. This study provides fundamental information on members of the bamboo HSF gene family and lays a foundation for further study of their biological functions in the regulation of plant responses to adversity.

Unraveling Microbial Volatile Elicitors Using a Transparent Methodology for Induction of Systemic Resistance and Regulation of Antioxidant Genes at Expression Levels in Chili against Bacterial Wilt Disease

“…The relative expression of HFSC1 gene was lower in roots than in leaf tissue. This may be because of the direct exposure of leaves to heat in glasshouse conditions [ 107 , 108 , 109 ]. The highest relative expression of HFSC1 in leaves was recorded after treatment with KA9 + PDS1 + RS compared to the UBI3.…”

Entailing the Next-Generation Sequencing and Metabolome for Sustainable Agriculture by Improving Plant Tolerance

“…Furthermore, abiotic stresses create a serious impact on bamboo growth and development [213]. Access to the moso bamboo genome [210,211] provides a chance to many researchers for genome-wide classifications of TFs such as aquaporin, AAAP, UBP, IQD, HD-Zip, Hexokinase, Aux/IAA and ARF, NAC, PeUGE, HSF, and CONSTANS-like in moso bamboo [188,[214][215][216][217][218][219][220][221][222][223]. Genome-wide classifications of TFs' families in moso bamboo have been carried out and have demonstrated a limited molecular characterization in model plants by exogenous gene transfer in rice and Arabidopsis and the expression profile against stresses [224][225][226].…”

“…Furthermore, abiotic stresses create a serious impact on bamboo growth and development [ 213 ]. Access to the moso bamboo genome [ 210 , 211 ] provides a chance to many researchers for genome-wide classifications of TFs such as aquaporin, AAAP, UBP, IQD, HD-Zip, Hexokinase, Aux/IAA and ARF, NAC, PeUGE, HSF, and CONSTANS-like in moso bamboo [ 188 , 214 , 215 , 216 , 217 , 218 , 219 , 220 , 221 , 222 , 223 ].…”

Characteristics and Regulating Roles of Wheat TaHsfA2-13 in Abiotic Stresses

“…In contrast to the few Hsfs in yeast and mammals, the plant Hsf families contain a large number of Hsf members, forming a complex plant-specific superfamily that is widespread in many species ( Guo et al, 2016 ). There are 21 Hsfs in Arabidopsis , 24 in tomato, 25 in pepper, 41 in bamboo, 52 in soybean, and 82 in wheat ( Scharf et al, 2012 ; Fragkostefanakis et al, 2015 ; Guo et al, 2015 ; Duan et al, 2019 ; Huang et al, 2021 ). Compared to other plant species, the large number of Hsfs in wheat makes the corresponding research more complicated.…”