Genome-wide genetic diversity and population structure of Garcinia kola (Heckel) in Benin using DArT-Seq technology

Dadjo, Colombe; Nyende, Aggrey Bernard; Yao, Nasser; Ngeno, Kiplangat; Assogbadjo, Achille Ephrem

doi:10.1371/journal.pone.0238984

Cited by 7 publications

(16 citation statements)

References 32 publications

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“…South region populations showed higher genetic diversity than those from the Central part. Populations of G. kola from Benin also revealed low levels of genetic diversity [ 25 ], which the latter authors attribute to the effect of domestication. However, the present study sampled wild individuals and did not discover any negative effect of domestication on genetic diversity ( Table 9 ).…”

Section: Discussionmentioning

confidence: 99%

“…Existing information gaps are limiting the potential improvement of the tree [ 23 ]. Only a few studies have focused on the morphological [ 6 , 23 , 24 ] and genetic diversity of G. kola [ 16 , 25 ]; however, none of them combined both approaches. Morphological diversity deals with the variation in quantifiable phenotypic traits such as fruit and seed weight.…”

Section: Introductionmentioning

confidence: 99%

“…In recent studies using random amplified polymorphic DNA (RAPD) markers, Olawuyi and Azeez [ 16 ] reported the existence of two distinct accessions of G. kola in Nigeria, suggesting that adaptation to local climatic factors has a significant role in the genetic diversity of the species. Similarly, Dadjo et al [ 25 ] reported low levels of overall genetic diversity in Benin populations using single-nucleotide polymorphism (SNP) markers, probably following a decrease in tree population size. However, no studies so far have focused on the genetic characterisation of the species in Cameroon.…”

Section: Introductionmentioning

confidence: 99%

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Domestication Potential of Garcinia kola Heckel (Clusiaceae): Searching for Diversity in South Cameroon

Maňourová

Chinheya

Kalousová

et al. 2023

Plants

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Seeds and bark of Garcinia kola Heckel (Clusiaceae) are popular products in West and Central Africa. Despite the tree’s economic and cultural importance, little is known about its phenotypic and genotypic variation. This study characterised the morphological and genetic diversity of G. kola in South Cameroon, searching for traits and populations that might be used for domestication. Morphological assessment and amplified fragment length polymorphism (AFLP) markers were applied to characterise diversity among geographic populations from Central and South regions, and between managed and wild trees. AFLP-SURV and analysis of molecular variance results indicated that a major part of genetic diversity is harboured within populations rather than between them. Bayesian analysis, principal component analysis and t-SNE identified three clusters where Ebolowa emerged as the transition population combining features from both regions. Trees from the South demonstrated a higher incidence of domestication-related traits, showing higher genetic diversity compared to the Central region. This suggests that individuals from the South might be more suitable for selection as “elite trees” in future breeding strategies for the species. No significant differences in phenotype and genotype were revealed between wild and managed populations, suggesting G. kola is still in the early stages of its domestication process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Domestication Potential of Garcinia kola Heckel (Clusiaceae): Searching for Diversity in South Cameroon

Maňourová

Chinheya

Kalousová

et al. 2023

Plants

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“…Different trends were also observed among natural stands of species other than palms. For example, Dadjo et al (2020) found only 0.86% genetic differentiation among sites in Garcinia kola (Heckel), which is an extensively utilized tree, and Wojnicka-Półtorak et al (2017) reported 13% genetic differentiation among age classes in natural populations of P. sylvestris.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Medemia argun (Mart.) Wurttenb. ex H.Wendl. Based on Genome-Wide Markers

Elshibli

Korpelainen

2021

Front. Ecol. Evol.

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Medemia argun is a wild, dioecious palm, adapted to the harsh arid environment of the Nubian Desert in Sudan and southern Egypt. There is a concern about its conservation status, since little is known about its distribution, abundance, and genetic variation. M. argun grows on the floodplains of seasonal rivers (wadis). The continuing loss of suitable habitats in the Nubian Desert is threatening the survival of this species. We analyzed the genetic diversity, population genetic structure, and occurrence of M. argun populations to foster the development of conservation strategies for M. argun. Genotyping-by-sequencing (GBS) analyses were performed using a whole-genome profiling service. We found an overall low genetic diversity and moderate genetic structuring based on 40 single-nucleotide polymorphisms (SNPs) and 9,866 SilicoDArT markers. The expected heterozygosity of the total population (HT) equaled 0.036 and 0.127, and genetic differentiation among populations/groups (FST) was 0.052 and 0.092, based on SNP and SilicoDArT markers, respectively. Bayesian clustering analyses defined five genetic clusters that did not display any ancestral gene flow among each other. Based on SilicoDArT markers, the results of the analysis of molecular variance (AMOVA) confirmed the previously observed genetic differentiation among generation groups (23%; p < 0.01). Pairwise FST values indicated a genetic gap between old and young individuals. The observed low genetic diversity and its loss among generation groups, even under the detected high gene flow, show genetically vulnerable M. argun populations in the Nubian Desert in Sudan. To enrich and maintain genetic variability in these populations, conservation plans are required, including collection of seed material from genetically diverse populations and development of ex situ gene banks.

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“…Garcinia kola Heckel (Clusiaceae/Guttiferae) is an African rain forest plant whose seeds, best known as “Onie” in Fang-Beti languages (Cameroon, Gabon, and Equatorial Guinea), “Orogbo” in Yoruba language (Nigeria), or bitter kola, all over West Africa, are eaten recreationally and used in traditional medicine to treat T1D and malaria [ 18 – 20 ]. Early reports suggested that G. kola seeds have anti-inflammatory, antidiabetic, and neuroprotective properties [ 21 – 23 ].…”

Section: Introductionmentioning

confidence: 99%

An Eluate of the Medicinal Plant Garcinia kola Displays Strong Antidiabetic and Neuroprotective Properties in Streptozotocin-Induced Diabetic Mice

Etet

Hamza

Eltahir³

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Scope. The neuroprotective properties of the antidiabetic plant Garcinia kola have been reported. Here, we performed a motor sign prevention-guided fractionation of G. kola extract in diabetic mice to unravel the components of the most active subfraction, given the potential for the development of drugs with antidiabetic and neuroprotective properties. Materials and Methods. G. kola methanolic extract was fractionated using increasingly polar solvents. Fractions were administered to streptozotocin (STZ)-induced diabetic mice until marked motor signs developed in diabetic controls. Fine motor skills indicators were measured in the horizontal grid test (HGT) to confirm the prevention of motor disorders in treated animals. Column chromatography was used to separate the most active fraction, and subfractions were tested in turn in the HGT. Gas chromatography-mass spectrometry (GC-MS) technique was used to assess the components of the most active subfraction. Results. Treatment with ethyl acetate fraction and its fifth eluate (F5) preserved fine motor skills and improved the body weight and blood glucose level. At dose 1.71 mg/kg, F5 kept most parameters comparable to the nondiabetic vehicle group values. GC-MS chromatographic analysis of F5 revealed 36 compounds, the most abundantly expressed (41.8%) being the β-lactam molecules N-ethyl-2-carbethoxyazetidine (17.8%), N,N-dimethylethanolamine (15%), and isoniacinamide (9%). Conclusions. Our results suggest that subfraction F5 of G. kola extract prevented the development of motor signs and improved disease profile in an STZ-induced mouse model of diabetic encephalopathy. Antidiabetic activity of β-lactam molecules accounted at least partly for these effects.

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Genome-wide genetic diversity and population structure of Garcinia kola (Heckel) in Benin using DArT-Seq technology

Cited by 7 publications

References 32 publications

Domestication Potential of Garcinia kola Heckel (Clusiaceae): Searching for Diversity in South Cameroon

Domestication Potential of Garcinia kola Heckel (Clusiaceae): Searching for Diversity in South Cameroon

Genetic Diversity and Population Structure of Medemia argun (Mart.) Wurttenb. ex H.Wendl. Based on Genome-Wide Markers

An Eluate of the Medicinal Plant Garcinia kola Displays Strong Antidiabetic and Neuroprotective Properties in Streptozotocin-Induced Diabetic Mice

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