Genome-wide gene expression responses to experimental manipulation of Saccharomyces cerevisiae repressor activator protein 1 (Rap1) expression level

Kalra, Shalini; Peyser, Rubye Dimitri; Ho, Josephine; Babbin, C.; Bohan, N.; Cortés, Alba; Erley, J.; Fatima, Mahpara; Flinn, J.; Horwitz, E. P.; Hsu, R.; Lee, W.; Lu, V.; Narch, A.; Navas, D.; Okoroafor, K.; Ouanemalay, E.; Ross, Stuart; Sowole, F.; Specht, E.; Woo, Jerry K. K.; Yu, Kexin; Coolon, Joseph D.

doi:10.1016/j.ygeno.2023.110625

Cited by 3 publications

(2 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, TbRAP1 does not seem to directly regulate the parasite metabolism, even though RNAseq analysis suggests that TbRAP1 is required for the fulllevel expression of multiple ribosomal protein genes [105][106][107]. In comparison, ScRap1 is a transcription regulator for ribosomal protein and glycolysis protein genes [97,127], while mouse RAP1 deficiency leads to decreased activation of PPARα and PGC1α, presumably through regulating the expression of genes encoding these enzymes [281,282].…”

Section: Competition Between Tbrap1's Dna and Rna Binding Activities ...mentioning

confidence: 98%

“…ScRap1 is an essential protein, and the C-terminus of its DNA binding domain is indispensable [126]. A recent genomic study comprehensively summarized ScRAP1's role as a transcription regulator: ScRap1 activates ribosomal protein and RNR genes and suppresses glycolysis genes and homothallic mating loci [127]. On the other hand, ScRap1's effect on subtelomeric gene expression is predominantly repressive [120,121,[128][129][130].…”

Section: Scrap1 Is a Key Player Of Telomeric Silencing In S Cerevisiaementioning

confidence: 99%

See 1 more Smart Citation

Unwrap RAP1’s Mystery at Kinetoplastid Telomeres

2024

Biomolecules

View full text Add to dashboard Cite

Although located at the chromosome end, telomeres are an essential chromosome component that helps maintain genome integrity and chromosome stability from protozoa to mammals. The role of telomere proteins in chromosome end protection is conserved, where they suppress various DNA damage response machineries and block nucleolytic degradation of the natural chromosome ends, although the detailed underlying mechanisms are not identical. In addition, the specialized telomere structure exerts a repressive epigenetic effect on expression of genes located at subtelomeres in a number of eukaryotic organisms. This so-called telomeric silencing also affects virulence of a number of microbial pathogens that undergo antigenic variation/phenotypic switching. Telomere proteins, particularly the RAP1 homologs, have been shown to be a key player for telomeric silencing. RAP1 homologs also suppress the expression of Telomere Repeat-containing RNA (TERRA), which is linked to their roles in telomere stability maintenance. The functions of RAP1s in suppressing telomere recombination are largely conserved from kinetoplastids to mammals. However, the underlying mechanisms of RAP1-mediated telomeric silencing have many species-specific features. In this review, I will focus on Trypanosoma brucei RAP1’s functions in suppressing telomeric/subtelomeric DNA recombination and in the regulation of monoallelic expression of subtelomere-located major surface antigen genes. Common and unique mechanisms will be compared among RAP1 homologs, and their implications will be discussed.

show abstract