Genome-wide functional analysis on the molecular mechanism of specifically biosynthesized fluorescence Eu complex

Abstract

Fluorescence imaging as an attractive diagnostic technique is widely employed for early diagnosis of cancer. Self-biosynthesized fluorescent Eu complex in situ in Hela cells have realized specifically and accurately fluorescence imaging for cancer cells. But the molecular mechanism of the in situ biosynthesized process is still unclear. In order to reveal this mechanism, we have investigated whole-genome expression profiles with cDNA microarray, incubated with Eu solution in Hela cells for 24 h. Methylthiazoltetrazolium (MTT) assay and laser confocal fluorescence microscopy study showed the low cytotoxicity and specifically fluorescence imaging of Eu complex in Hela cells. It is observed that 563 up-regulated genes and 274 down-regulated genes were differentially expressed. Meanwhile, quantitative RT-PCR was utilized to measure the expression of some important genes, which validated the results of microarray data analysis. Besides, GO analysis showed that a wide range of differential expression functional genes involved in three groups, including cellular component, molecular function and cellular biological process. It was evident that some important biological pathways were apparently affected through KEGG pathway analysis, including focal adhesion pathway and PI3K (phosphatidylinositol 3′ -kinase)-Akt signaling pathway, which can influence glycolytic metabolism and NAD(P)H-oxidases metabolic pathway.

Hybrid nanomaterials-based biomedical phototheranostic platforms

“…In order to further reveal the molecular mechanism of in situ biosynthesis, cDNA microarray was used to study the whole genome expression, and GO analysis and KEGG pathway analysis were performed on the differentially expressed genes. We found that in the process of in situ biosynthesis of fluorescent nanocomplexes, there are several important biological pathways involved, including the focal plaque pathway and the PI3K (phosphatidylinositol 3 ′ -kinase)-Akt signaling pathway, which can affect glycolytic metabolism and NAD(P)H-oxidase metabolism [215]. Based on genomics studies, the molecular mechanism of the differentially expressed proteins in the process of in situ synthesis in cells was further studied by using the iso-heavy isotopic multi-label relative quantitative proteomics (iTRAQ) [199].…”