Genome-wide expression profiling and phenotypic evaluation of European maize inbreds at seedling stage in response to heat stress

Frey, Felix P.; Urbany, Claude; Hüttel, Bruno; Reinhardt, Richard; Stich, Benjamin

doi:10.1186/s12864-015-1282-1

Cited by 84 publications

(93 citation statements)

References 52 publications

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“…Substantial genetic variability between genotypes in contrast to treatment effects, as observed in our experiments, was also described in previous studies on the diversity of European flint landraces accessed through doubled haploids [4,8,27]. Similar results were obtained in a set of diverse European flint and dent inbred lines subjected to heat stress where the transcriptomic responses between heat tolerant and susceptible genotypes were highly divergent [33]. Likewise, in a transcriptome study with the US inbred lines B73 and Mo17 and their reciprocal F 1 -hybrids genotypic differences were much more pronounced than differences between water deficit stress and control conditions (Marcon et al, 2017).…”

Section: Transcriptomic Diversity With Respect To Cold Tolerancesupporting

confidence: 90%

Transcriptomic diversity in seedling roots of European flint maize in response to cold

et al. 2020

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Background: Low temperatures decrease the capacity for biomass production and lead to growth retardation up to irreversible cellular damage in modern maize cultivars. European flint landraces are an untapped genetic resource for genes and alleles conferring cold tolerance which they acquired during their adaptation to the agroecological conditions in Europe. Results: Based on a phenotyping experiment of 276 doubled haploid lines derived from the European flint landrace "Petkuser Ferdinand Rot" diverging for cold tolerance, we selected 21 of these lines for an RNA-seq experiment. The different genotypes showed highly variable transcriptomic responses to cold. We identified 148, 3254 and 563 genes differentially expressed with respect to cold treatment, cold tolerance and growth rate at cold, respectively. Gene ontology (GO) term enrichment demonstrated that the detoxification of reactive oxygen species is associated with cold tolerance, whereas amino acids might play a crucial role as antioxidant precursors and signaling molecules. Conclusion: Doubled haploids representing a European maize flint landrace display genotype-specific transcriptome patterns associated with cold response, cold tolerance and seedling growth rate at cold. Identification of cold regulated genes in European flint germplasm, could be a starting point for introgressing such alleles in modern breeding material for maize improvement.

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Section: Transcriptomic Diversity With Respect To Cold Tolerancesupporting

confidence: 90%

Transcriptomic diversity in seedling roots of European flint maize in response to cold

et al. 2020

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“…In contrast to drought and salt stress, SUT1 was found to be significantly down-regulated under heat stress in the tested monocots, while SUT4 expression showed the same response. This result matches the observed strong reduction of growth in all tissues observed in heat-stressed grasses (Kotak et al, 2007;Frey et al, 2015).…”

Section: Sut Regulation Is Part Of the Abiotic Stress Responsesupporting

confidence: 78%

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

et al. 2017

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Suc transporters (SUTs) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. While a function could be assigned to many members of the SUT family, almost no information is available on their regulation. Here, the transcriptional regulation of SUTs in response to various environmental stimuli in the leaves of five dicots (Arabidopsis Extensive data on expression of SUTs in relation to changes of environmental conditions were obtained through a global analysis of 168 transcriptomics data sets. Results were validated by quantitative PCR measurements and extended by the measurement of photosynthesis rate and phloem sugar content to draw insight on the correlation of SUT expression and sugar export from leaves. For the apoplasmic phloem loaders, a clear difference in transcriptional regulation in response to different environmental stimuli was observed. The consistent patterns of SUT expression under abiotic stress indicates which types of SUTs are involved in the regulation of leaf sugar status and in stress signaling. Furthermore, it is shown that down-regulation of phloem loading is likely to be caused by transcriptional regulation of SUTs, while up-regulation depends on post-transcriptional regulation. In poplar, expression of PtaSUT4 was found to consistently respond to environmental stimuli, suggesting a significant role in the regulation of sugar export from leaves in this passive symplasmic phloem loader.

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“…To verify RNA-seq results, possible leaf curling- and ABA signaling-related genes including GRMZM2G047065 , AC208201.3_FG002 , GRMZM2G010855 , GRMZM2G057959 , GRMZM2G144224 and GRMZM2G154987 were selected for qRT-PCR analysis. Expression values by qRT-PCR were calculated by relative expression of genes to ZmACT1 (GRMZM2G126010 )47. To investigate the functional relevance of differentially expressed genes of each type between WT and RNAi line, we performed GO enrichment analysis with complete GO assignments from AgriGO (http://bioinfo.cau.edu.cn/agriGO/).…”

Section: Methodsmentioning

confidence: 99%

The Core Subunit of A Chromatin-Remodeling Complex, ZmCHB101, Plays Essential Roles in Maize Growth and Development

Jiang

et al. 2016

Sci Rep

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ATP-dependent chromatin remodeling complexes play essential roles in the regulation of diverse biological processes by formulating a DNA template that is accessible to the general transcription apparatus. Although the function of chromatin remodelers in plant development has been studied in A. thaliana, how it affects growth and development of major crops (e.g., maize) remains uninvestigated. Combining genetic, genomic and bioinformatic analyses, we show here that the maize core subunit of chromatin remodeling complex, ZmCHB101, plays essential roles in growth and development of maize at both vegetative and reproductive stages. Independent ZmCHB101 RNA interference plant lines displayed abaxially curling leaf phenotype due to increase of bulliform cell numbers, and showed impaired development of tassel and cob. RNA-seq-based transcriptome profiling revealed that ZmCHB101 dictated transcriptional reprogramming of a significant set of genes involved in plant development, photosynthesis, metabolic regulation, stress response and gene expressional regulation. Intriguingly, we found that ZmCHB101 was required for maintaining normal nucleosome density and 45 S rDNA compaction. Our findings suggest that the SWI3 protein, ZmCHB101, plays pivotal roles in maize normal growth and development via regulation of chromatin structure.

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Genome-wide expression profiling and phenotypic evaluation of European maize inbreds at seedling stage in response to heat stress

Cited by 84 publications

References 52 publications

Transcriptomic diversity in seedling roots of European flint maize in response to cold

Transcriptomic diversity in seedling roots of European flint maize in response to cold

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

The Core Subunit of A Chromatin-Remodeling Complex, ZmCHB101, Plays Essential Roles in Maize Growth and Development

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