Genome-Wide DNA Methylation Profiling in Dietary Intervention Studies: a User’s Perspective

Gerhäuser, Clarissa; Heilmann, Katharina; Pudenz, Maria

doi:10.1007/s40495-014-0001-y

Cited by 2 publications

(1 citation statement)

References 84 publications

(89 reference statements)

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“…23 Both of them cooperate to methylate the promoter regions of the OCT3/4 and Nanog genes during the differentiation of embryonic carcinoma cells and ES cells, and the removal of them can lead to insufficient methylation and disordered OCT3/4 expression. 24,25 Here, we demonstrated that in A549 and NCI-H226 lung cells the activity of the maintenance methyltransferase DNMT1 and DNMT3b decreased after 5-Fu/CDDP treatment, but DNMT3a in NCI-H226 cells significantly increased. DNMT3a was increased in the NCI-H226 cell line, which may be related to the interference of other factors in the NCI-H226 cell line.…”

Section: The Expression Of H3k9me3 and H3k9ace By Chipmentioning

confidence: 70%

Epigenetic Mechanism of Enrichment of A549 Lung Cancer Stem Cells with 5-Fu

Cao¹,

Shi²,

B³

et al. 2021

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Background The influence of 5-fluorouracil (5-Fu) and cisplatin (CDDP) on the A549 and NCI-H226 cells was studied, and the epigenetic mechanism of enrichment of A549 lung cancer stem cells with 5-Fu was explored. Materials and Methods The cell proliferation of both A549 and NCI-H226 was detected by BrdU assay, and apoptosis condition was measured by flow cytometric analysis. The expressions of OCT3/4 and Nanog in cells treated with 5-Fu or CDDP were measured by immunofluorescence, Western blot and qPCR. qPCR was also performed to determine the relative expression of methyltransferase genes and miRNA. Sequencing after bisulfite treatment (BSP) was employed to detect the methylation of OCT3/4 promoter in A549 cells. And ChIP was conducted to detect the expression of H3K9Me3 and H3K9Ace. Results Both 5-Fu and CDDP result in the apoptosis of A549 and NCI-H226 cells and improve the expressions of has-miR-134 and has-miR-296. However, 5-Fu enhances the expression of OCT3/4 in A549 cells, and the change of methyltransferase genes and BSP results suggested some genetic differences between CDDP and 5-Fu treatment in A549 cells. ChIP assay showed that the expression of H3K9Me3 significantly decreased and H3K9Ace significantly increased in A549 cells. Conclusion The enrichment effect of CDDP on A549 and NCI-H226 carcinoma stem cells is inconsistent with the enrichment effect of 5-Fu. The enrichment of A549 lung cancer stem cells with 5-Fu might be related to the methylation of OCT3/4 promoter and the expression of H3K9Me3 and H3K9Ace.

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Section: The Expression Of H3k9me3 and H3k9ace By Chipmentioning

confidence: 70%

Epigenetic Mechanism of Enrichment of A549 Lung Cancer Stem Cells with 5-Fu

Cao¹,

Shi²,

B³

et al. 2021

OTT

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Next-generation sequencing approaches for the study of genome and epigenome toxicity induced by sulfur mustard

et al. 2018

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Sulfur mustard (SM) is an extensive nucleophilic and alkylating agent that targets different tissues. The genotoxic property of SM is the most threatening effect, because it is associated with detrimental inflammations and susceptibility to several kinds of cancer. Moreover, SM causes a wide variety of adverse effects on DNA which result in accumulation of DNA adducts, multiple mutations, aneuploidies, and epigenetic aberrations in the genome. However, these adverse effects are still not known well, possibly because no valid biomarkers have been developed for detecting them. The advent of next-generation sequencing (NGS) has provided opportunities for the characterization of these alterations with a higher level of molecular detail and cost-effectivity. The present review introduces NGS approaches for the detection of SM-induced DNA adducts, mutations, chromosomal structural variation, and epigenetic aberrations, and also comparing and contrasting them with regard to which might be most advantageous.

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Genome-Wide DNA Methylation Profiling in Dietary Intervention Studies: a User’s Perspective

Cited by 2 publications

References 84 publications

Epigenetic Mechanism of Enrichment of A549 Lung Cancer Stem Cells with 5-Fu

Epigenetic Mechanism of Enrichment of A549 Lung Cancer Stem Cells with 5-Fu

Next-generation sequencing approaches for the study of genome and epigenome toxicity induced by sulfur mustard

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