Genome-wide comprehensive analysis of transcriptomes and small RNAs offers insights into the molecular mechanism of alkaline stress tolerance in a citrus rootstock

Abstract

Alkaline stress has serious-negative effects on citrus production. Ziyang xiangcheng ( Citrus junos Sieb. ex Tanaka) (Cj) is a rootstock that is tolerant to alkaline stress and iron deficiency. Trifoliate orange ( Poncirus trifoliata (L.) Raf.) (Pt), the most widely used rootstock in China, is sensitive to alkaline stress. To investigate the molecular mechanism underlying the tolerance of Cj to alkaline stress, next-generation sequencing was employed to profile the root transcriptomes and small RNAs of Cj and Pt seedlings that were cultured in nutrient solutions along a three pH gradient. This two-level regulation data set provides a system-level view of molecular events with a precise resolution. The data suggest that the auxin pathway may play a central role in the inhibitory effect of alkaline stress on root growth and that the regulation of auxin homeostasis under alkaline stress is important for the adaptation of citrus to alkaline stress. Moreover, the jasmonate (JA) pathway exhibits the opposite response to alkaline stress in Cj and Pt and may contribute to the differences in the alkaline stress tolerance and iron acquisition between Cj and Pt. The dataset provides a wealth of genomic resources and new clues to further study the mechanisms underlying alkaline stress resistance in Cj.

Investigation of the cause of reduced sugar content in Kiyomi tangor fruit of Ziyang xiangcheng (Citrus junos Sieb. ex Tanaka) rootstock

“…Rootstock is an important part of the Citrus industry as it influences scion characteristics such as fruit quality, canopy size, and resistance 8,9 . Previous studies have demonstrated that Citrus -grafted seedlings with Pt as rootstock were prone to nutrient deficiency and growth retardation in calcareous soils 10,11 . However, Ziyang xiangcheng ( Citrus junos Sieb.…”

“…However, Ziyang xiangcheng ( Citrus junos Sieb. ex Tanaka) (Cj) is a local Citrus rootstock originating from Southwest China with excellent disease-, alkali-, drought-, and cold-resistant characteristics 10 . Additionally, Cj is more alkali-resistant than Pt.…”

“…Moreover, It was not until the soil pH increased to 8.3 that the leaf etiolation appeared on Citrus with Cj as rootstock 12 . Therefore, in recent years, Cj has been used as a rootstock in more and more scion varieties in China due to its excellent tolerance to alkaline stress and iron deficiency 10 . However, we found that the sugar content of K .…”

Influence of Citrus Scion/Rootstock Genotypes on Arbuscular Mycorrhizal Community Composition under Controlled Environment Condition

“…Citrus is a woody perennial plant, and it is widely planted all over the world in a form of graft. For instance, the commercial citrus cultivars Newhall sweet orange and Mandarin as scion are grafted onto the rootstocks Poncirus and Citrange , which is of significant importance for increasing the fruit’s quality and its resistance to multiple biotic and abiotic stresses [ 48 , 49 , 50 , 51 , 52 ]. However, how scion and rootstock combinations affect the AMF community in citrus roots is still poorly understood.…”

Resveratrol Alleviates the KCl Salinity Stress of Malus hupenensis Rhed. Seedlings by Regulating K+/Na+ Homeostasis, Osmotic Adjustment, and Reactive Oxygen Species Scavenging

“…Applying exogenous substances effectively alleviates salt stress [29]. These substances are commonly divided into the following three categories: The first category is the plant growth regulators, such as jasmonate (JA), cytokinin (CTK), and abscisic acid (ABA) [4,30,31]. JA has a positive regulatory role in plant resistance to salt stress, and its exogenous application can improve the salt tolerance of Arabidopsis, tomatoes, and rice [32,33,34].…”