Genome-wide comparative analysis of long-chain acyl-CoA synthetases (LACSs) gene family: A focus on identification, evolution and expression profiling related to lipid synthesis

Ayaz, Asma; Saqib, Saddam; Huang, Haohao; Zaman, Wajid; Lü, Shiyou; Zhao, Huayan

doi:10.1016/j.plaphy.2021.01.042

Cited by 49 publications

(48 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A total of 19 LACSs were identified from G. max based on their homologies to the nine known LACS from Arabidopsis. According to the classification criteria used for Arabidopsis and other plant species in previous studies [12,15], the LACSs were grouped into 6 clades with well-supported bootstrap values. GmLACS genes were placed in each clade with respective Arabidopsis LACS (Figure 1).…”

Section: Identification and Homology Analysis Of Gmlacssmentioning

confidence: 99%

“…These results also confirmed that GmLACS2-3 participates in cutin biosynthesis. Recently, we found that AtLACS2 is also involved in suberin synthesis [15]. To determine whether GmLACS2-3 plays a similar role in the synthesis of root suberin, we measured the suberin components of GmLACS2-3 OE1, GmLACS2-3 OE2, and Col-0 as well.…”

Section: Overexpression Of Gmlacs2-3 In Arabidopsis Can Promote the Synthesis Of Both Cutin And Suberin But Not Waxmentioning

confidence: 99%

“…AtLACS2 is also an ER-localized gene but plays an important role in cutin synthesis, and its loss of activity resulted in a significant reduction in cutin levels, resulting in lower membrane permeability and altered plant responses to abiotic and biotic stressors [13,14]. Moreover, it was also identified to be involved in suberin synthesis since the disruption of AtLACS2 activity drastically reduced suberin amounts [15]. Suberin is mainly derived from long-chain aliphatic acids (>C16), the components of which are somewhat similar to cutin, thus partly sharing a biosynthesis pathway [9].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance

Ayaz

Huang

Zheng

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Cutin and wax are the main precursors of the cuticle that covers the aerial parts of plants and provide protection against biotic and abiotic stresses. Long-chain acyl-CoA synthetases (LACSs) play diversified roles in the synthesis of cutin, wax, and triacylglycerol (TAG). Most of the information concerned with LACS functions is obtained from model plants, whereas the roles of LACS genes in Glycine max are less known. Here, we have identified 19 LACS genes in Glycine max, an important crop plant, and further focused our attention on 4 LACS2 genes (named as GmLACS2-1, 2, 3, 4, respectively). These GmLACS2 genes display different expression patterns in various organs and also show different responses to abiotic stresses, implying that these genes might play diversified functions during plant growth and against stresses. To further identify the role of GmLACS2-3, greatly induced by abiotic stresses, we transformed a construct containing its full length of coding sequence into Arabidopsis. The expression of GmLACS2-3 in an Arabidopsis atlacs2 mutant greatly suppressed its phenotype, suggesting it plays conserved roles with that of AtLACS2. The overexpression of GmLACS2-3 in wild-type plants significantly increased the amounts of cutin and suberin but had little effect on wax amounts, indicating the specific role of GmLACS2-3 in the synthesis of cutin and suberin. In addition, these GmLACS2-3 overexpressing plants showed enhanced drought tolerance. Taken together, our study deepens our understanding of the functions of LACS genes in different plants and also provides a clue for cultivating crops with strong drought resistance.

show abstract

Section: Identification and Homology Analysis Of Gmlacssmentioning

confidence: 99%

Section: Overexpression Of Gmlacs2-3 In Arabidopsis Can Promote the Synthesis Of Both Cutin And Suberin But Not Waxmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance

Ayaz

Huang

Zheng

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The identification and classification of plants, the determination of broader biotic/genetic diversity, and the characterization of reactions and resistance against stress factors are often based on investigations of morphological features, especially microscopic level analyses (Al‐Ashkar et al, 2020; Ayaz et al, 2021; Ayaz, Zaman, Saqib, Ullah, & Mahmood, 2020; Hu et al, 2020; Silva et al, 2020). Since gypsum‐based edaphic endemics often have substrate specific physiological mechanisms or strategies to cope with the harsh conditions that are characteristic the soil in which grow, these extreme features are expected to be reflected in the morphology and anatomy of gypsophytes.…”

Section: Introductionmentioning

confidence: 99%

Micromorphological considerations on Alyssum nezaketiae Aytaç & H. Duman (Brassicaceae), endemic to gypsum habitats from Turkey: An electron microscopic study

Kayabaş

2021

Microscopy Res & Technique

View full text Add to dashboard Cite

Alyssum nezaketiae is a local endemic species in gypsum habitats of Çankırı province in Turkey. In this study, I used field collected specimens of A. nezaketiae in a scanning electron microscopy examination of vegetative and reproductive structures of A. nezaketiae. The objective of the study was to investigate the micromorphological properties that define the species and that represent its adaptation(s) to the extreme environmental conditions posed by gypsum habitats. The epidermis had one to two rows at transverse sections of leaf and stem. Cortex was composed six to eight rows parenchyma cells at stem. A number of adaptations to xeric habitats were observed, including: dense silvery lepidote hairs on leaves and stems, small leaves, stomata on the lower surfaces of the leaves occurring in stomatal crypts, intense lignification in the central cylinder of the stems, and bundles of sclerenchyma in the pith region of stems. These can be interpreted as gypsophytic characteristics associated with physiological drought. Micromorphological properties of plant parts are discussed in relation to environmental conditions and possible constraint factors occurring in these gypsum habitats. Furthermore, these micromorphological studies contribute important taxonomic characters that are needed to help identify a species whose habitat is under threat from a variety of human activities.

show abstract

“…In Arabidopsis, LACS enzymes are known to 'activate' lipid metabolism by generating fatty acyl-CoAs that are subsequently used in cutin and wax biosynthesis, amongst other processes (Shockey et al, 2002;Zhao et al, 2019). Originally found to only be involved in cutin monomer biosynthesis (Bessire et al, 2007;Schnurr et al, 2004),the lacs2 mutant was recently found to reduce the total suberin polymer in the root endodermis by almost half (Ayaz et al, 2021). In terms of suberin biosynthesis, the LACSgenerated fatty acyl thioesters at the ER have three possible fates: the acyl elongation pathway, the acyl reduction pathway, or the acyl oxidation pathway (Vishwanath et al, 2015).…”

Section: 5-suberin Biosynthesis Transport and Assemblymentioning

confidence: 99%

Transcriptional Regulators of Suberin Biosynthesis in Arabidopsis thaliana

Garant¹

View full text Add to dashboard Cite

Like all vascular plants, the root endodermis of Arabidopsis thaliana contains aliphatic suberin, a cell wall-associated hydrophobic polymer that helps direct the symplastic movement of water and solutes into the central vasculature. The enzymes involved in suberin biosynthesis are relatively well characterized, but there are many gaps in our knowledge on how suberin biosynthesis is regulated. In this study, I demonstrate that Arabidopsis MYB41, MYB53, MYB92, and MYB93, which are R2R3 MYBtype transcription factors, can activate the transcription of suberin biosynthesis genes through their promoter (region 5' of the translation start site). Using a promoter: reporter deletion series, two regions 5-REFERENCES ..

show abstract

Genome-wide comparative analysis of long-chain acyl-CoA synthetases (LACSs) gene family: A focus on identification, evolution and expression profiling related to lipid synthesis

Cited by 49 publications

References 45 publications

Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance

Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance

Micromorphological considerations on Alyssum nezaketiae Aytaç & H. Duman (Brassicaceae), endemic to gypsum habitats from Turkey: An electron microscopic study

Transcriptional Regulators of Suberin Biosynthesis in Arabidopsis thaliana

Contact Info

Product

Resources

About