Genome-wide comparative analysis of four Indian Drosophila species

Abstract

Comparative analysis of multiple genomes of closely or distantly related Drosophila species undoubtedly creates excitement among evolutionary biologists in exploring the genomic changes with an ecology and evolutionary perspective. We present herewith the de novo assembled whole genome sequences of four Drosophila species, D. bipectinata, D. takahashii, D. biarmipes and D. nasuta of Indian origin using Next Generation Sequencing technology on an Illumina platform along with their detailed assembly statistics. The comparative genomics analysis, e.g. gene predictions and annotations, functional and orthogroup analysis of coding sequences and genome wide SNP distribution were performed. The whole genome of Zaprionus indianus of Indian origin published earlier by us and the genome sequences of previously sequenced 12 Drosophila species available in the NCBI database were included in the analysis. The present work is a part of our ongoing genomics project of Indian Drosophila species.

Illumina short-read sequencing data, de novo assembly and annotations of the Drosophila nasuta nasuta genome

“…Hence, this draft genome can help in understanding mechanisms of whole chromosome fusion and their maintenance during the early stages of divergence. Here, we present a high quality Illumina draft genome assembly for D. n. nasuta (Coorg strain, Mysore) with better N50 values and contiguity than the existing asembly [4] of samples collected from different geographical location.…”

Rapid Divergence of Key Spermatogenesis Genes in nasuta-Subgroup of Drosophila

“…We downloaded whole genome raw sequences of 35 strains of 11 Drosophila species of nasuta-subgroup (Table S1) from NCBI-SRA (Mai et al 2020;Mohanty and Khanna, 2017). Genome assemblies were built using UniCycler (Wick et al 2017), which assembled the sequences into scaffolds.…”