Genome-wide characterization of the UDP-glycosyltransferase gene family reveals their potential roles in leaf senescence in cotton

Chen, Yizhen; Fu, Mingchuan; Li, Hao; Wang, Liguo; Liu, Renzhong; Liu, Zhanji

doi:10.1016/j.ijbiomac.2022.10.047

Cited by 12 publications

(5 citation statements)

References 66 publications

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“…These two groups have been found in wheat, as well [54]. In N. benthamiana, 81.63% of the NbUGTs did not contain any introns or contained only one intron, which is consistent with the number of UGT gene introns in tomato, rice, and cotton [51,53,55]. In this study, the website (http://cello.life.nctu.edu.tw/cello.html, accessed on 16 September 2022) was used to predict that these NbUGT genes were primarily located in the cytoplasm, whereas the UGT genes of wheat were mostly located in the cytoplasm, cell membrane, and chloroplast [54], and the UGT genes of cotton were mostly located in the cytosol and chloroplast [56].…”

Section: Discussionsupporting

confidence: 74%

“…In addition, the implementation of the Human Genome Project (HGP) and the rapid development of high-throughput sequencing technology have provided a vast amount of data for bioinformatics development. UGT genes have been identified and analyzed in many plants, such as maize, cotton, and tomato [ 51 , 52 , 53 ]. N. benthamiana is a heterotetraploid species with 19 chromosomes.…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in Nicotiana benthamiana

Wang,

Yang,

et al. 2024

Viruses

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Glycosylation, a dynamic modification prevalent in viruses and higher eukaryotes, is principally regulated by uridine diphosphate (UDP)-glycosyltransferases (UGTs) in plants. Although UGTs are involved in plant defense responses, their responses to most pathogens, especially plant viruses, remain unclear. Here, we aimed to identify UGTs in the whole genome of Nicotiana benthamiana (N. benthamiana) and to analyze their function in Chinese wheat mosaic virus (CWMV) infection. A total of 147 NbUGTs were identified in N. benthamiana. To conduct a phylogenetic analysis, the UGT protein sequences of N. benthamiana and Arabidopsis thaliana were aligned. The gene structure and conserved motifs of the UGTs were also analyzed. Additionally, the physicochemical properties and predictable subcellular localization were examined in detail. Analysis of cis-acting elements in the putative promoter revealed that NbUGTs were involved in temperature, defense, and hormone responses. The expression levels of 20 NbUGTs containing defense-related cis-acting elements were assessed in CWMV-infected N. benthamiana, revealing a significant upregulation of 8 NbUGTs. Subcellular localization analysis of three NbUGTs (NbUGT12, NbUGT16 and NbUGT17) revealed their predominant localization in the cytoplasm of N. benthamiana leaves, and NbUGT12 was also distributed in the chloroplasts. CWMV infection did not alter the subcellular localization of NbUGT12, NbUGT16, and NbUGT17. Transient overexpression of NbUGT12, NbUGT16, and NbUGT17 enhanced CWMV infection, whereas the knockdown of NbUGT12, NbUGT16 and NbUGT17 inhibited CWMV infection in N. benthamiana. These NbUGTs could serve as potential susceptibility genes to facilitate CWMV infection. Overall, the findings throw light on the evolution and function of NbUGTs.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in Nicotiana benthamiana

Wang,

Yang,

et al. 2024

Viruses

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“…Total RNA was isolated from the leaves. RT-qPCR was carried out as described by Chen et al (2022) [ 29 ]. The experiments were biologically repeated three times, and the relative expression levels of the GhACBP genes were calculated based on the 2 -ΔΔCt method [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton

Chen

et al. 2023

Genes

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Members of the acyl-CoA-binding protein (ACBP) gene family play vital roles in diverse processes related to lipid metabolism, growth and development, and environmental response. Plant ACBP genes have been well-studied in a variety of species including Arabidopsis, soybean, rice and maize. However, the identification and functions of ACBP genes in cotton remain to be elucidated. In this study, a total of 11 GaACBP, 12 GrACBP, 20 GbACBP, and 19 GhACBP genes were identified in the genomes of Gossypium arboreum, Gossypium raimondii, Gossypium babardense, and Gossypium hirsutum, respectively, and grouped into four clades. Forty-nine duplicated gene pairs were identified in Gossypium ACBP genes, and almost all of which have undergone purifying selection during the long evolutionary process. In addition, expression analyses showed that most of the GhACBP genes were highly expressed in the developing embryos. Furthermore, GhACBP1 and GhACBP2 were induced by salt and drought stress based on a real-time quantitative PCR (RT-qPCR) assay, indicating that these genes may play an important role in salt- and drought-stress tolerance. This study will provide a basic resource for further functional analysis of the ACBP gene family in cotton.

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“…Glycosylation, an important modi cation reaction in plants, plays a crucial role in growth and response to biotic and abiotic stresses, and it also enhances solubility, stability, transferability, and diversity of various plant secondary metabolites such as avonoids (Vogt et Glycosylation modi cation is catalyzed by glycosyltransferases (GTs), which are highly diverse, multifunctional enzymes belonging to a polymorphic gene family (Mackenzie et al 1997). According to the latest update on CAZy (http://www.cazy.org/Glycosyl Transferases.html), GTs can be classi ed into 115 distinct species based on amino acid sequence similarity, catalytic mechanism, and the presence of conserved sequence motifs (Chen et al 2022). Among these, the GT1 family, commonly referred to as uridine diphosphate glycosyltransferases (UGTs), represents the predominant group in plants and exerts signi cant in uence on plant growth and development (Yonekura et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of UGT gene family and their expression profiles analysis during fruit development in walnut (Juglans regia L.)

Shi,

Yao,

Yang

et al. 2024

Preprint

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Walnut (Juglans regia L.) possess the ability to prevent coronary heart disease and promote cardiovascular health, which can be attributed to their rich content of polyphenols, particularly flavonoids. The biosynthesis of flavonoids is reliant on the catalytic activity of uridine diphosphate glycosyltransferase (UGT). However, the identification of UGTs in walnut has not been reported. In the current study, a total of 124 UGT genes containing the PSPG box were identified from the walnut genome. Based on phylogenetic analysis, the 124 UGT could be classified into 16 distinct groups, which exhibited an uneven distribution across the 16 chromosomes. Subcellular localization prediction analysis revealed that approximately 78.23% of walnut UGT proteins were predominantly localized in the cytoplasmic compartment. Furthermore, motif annotation confirmed that motifs 1, 2, and 3 represented conserved structural features within UGT proteins, while interestingly, around 56.5% of walnut UGT members lacked introns. In conjunction with transcriptome analysis and quantitative expression, approximately 39% of UGT genes in walnut exhibited high expression levels during early fruit development. Correlation analysis between UGT genes expression and phenolic content in walnut indicated that JrUGT6, JrUGT38, JrUGT39, JrUGT58, JrUGT69, JrUGT75, and JrUGT82 might be involved in phenolic biosynthesis in walnut. This comprehensive study provides a comprehensive overview of the UGT genes in walnut, serving as a valuable reference and theoretical foundation for further investigations into the biological functions of JrUGTs in flavonoid biosynthesis.

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Genome-wide characterization of the UDP-glycosyltransferase gene family reveals their potential roles in leaf senescence in cotton

Cited by 12 publications

References 66 publications

Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in Nicotiana benthamiana

Genome-Wide Analysis and Identification of UDP Glycosyltransferases Responsive to Chinese Wheat Mosaic Virus Resistance in Nicotiana benthamiana

Molecular Characterization of the Acyl-CoA-Binding Protein Genes Reveals Their Significant Roles in Oil Accumulation and Abiotic Stress Response in Cotton

Genome-wide identification of UGT gene family and their expression profiles analysis during fruit development in walnut (Juglans regia L.)

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